Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,666,252 G→T S313I (AGC→ATC)  clcB → putative chloride:H(+) antiporter ClcB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,666,2520GT100.0% 269.9 / NA 76S313I (AGC→ATC) clcBputative chloride:H(+) antiporter ClcB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (45/31);  total (45/31)

CTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACC  >  NC_000913/1666122‑1666385
                                                                                                                                  |                                                                                                                                     
cTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCgg                                                                                                                              >  2:1329794/1‑140 (MQ=255)
cTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCgg                                                                                                                              >  1:704148/1‑140 (MQ=255)
  ttCCCTGTTCACACCTGCAGTGT‑GGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGgcgc                                                                                                                           <  1:194076/140‑1 (MQ=255)
     ccTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCAc                                                                                                                         <  1:817187/140‑1 (MQ=255)
        gTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCg                                                                                                                      >  2:927205/1‑140 (MQ=255)
          tCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGt                                                                                                                    >  2:917559/1‑140 (MQ=255)
          tCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGt                                                                                                                    <  2:166066/140‑1 (MQ=255)
          tCACACCTGCAGTGTGGGGCAACGACTATAGCACCGTACAACCCTTTTTAACCGCCCCCCCACTGTTAATGATCATTGCGGGGACCTCCCTTGGTAAACTGTTGGCCGTCCTGGCGAGTTTCGGTCCCGCCGCACCCGGt                                                                                                                    >  2:36930/1‑140 (MQ=255)
            acacCTGCAGTGTGGGGCAACGGCTATAGCACCGTGCAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTgg                                                                                                                  <  1:221817/140‑1 (MQ=255)
            acacCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTgg                                                                                                                  <  2:49645/140‑1 (MQ=255)
            acacCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTgg                                                                                                                  <  1:1308485/140‑1 (MQ=255)
            acacCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTgg                                                                                                                  <  1:750196/140‑1 (MQ=255)
            acacCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTgg                                                                                                                  <  1:560769/140‑1 (MQ=255)
             cacCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTggg                                                                                                                 <  1:917559/140‑1 (MQ=255)
             cacCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTggg                                                                                                                 <  2:851317/140‑1 (MQ=255)
                   cAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCttt                                                                                                           >  1:1425067/1‑140 (MQ=255)
                        tgGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACAcc                                                                                                      >  2:733638/1‑140 (MQ=255)
                        tgGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACAcc                                                                                                      >  2:1151034/1‑140 (MQ=255)
                        tgGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACAcc                                                                                                      >  1:663195/1‑140 (MQ=255)
                           ggCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGAc                                                                                                   <  2:704148/140‑1 (MQ=255)
                            gCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACg                                                                                                  <  1:1329794/140‑1 (MQ=255)
                              aaCGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCt                                                                                                <  1:36930/140‑1 (MQ=255)
                                cGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTAt                                                                                              >  2:904620/1‑140 (MQ=255)
                                      taGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCg                                                                                        >  1:1178248/1‑140 (MQ=255)
                                          aCCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGAATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCt                                                                                    >  1:81276/1‑140 (MQ=255)
                                           ccGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCtt                                                                                   >  1:564594/1‑140 (MQ=255)
                                            cGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTg                                                                                  >  1:1079224/1‑140 (MQ=255)
                                            cGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTg                                                                                  >  1:254095/1‑140 (MQ=255)
                                            cGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTg                                                                                  >  1:505400/1‑140 (MQ=255)
                                              tACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGcc                                                                                >  2:282739/1‑140 (MQ=255)
                                              tACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGcc                                                                                >  1:1281263/1‑140 (MQ=255)
                                              tACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGcc                                                                                >  1:79385/1‑140 (MQ=255)
                                              tACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGcc                                                                                >  2:677733/1‑140 (MQ=255)
                                               aCAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCa                                                                               <  1:677733/140‑1 (MQ=255)
                                                   tCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTgg                                                                           >  1:514294/1‑140 (MQ=255)
                                                    ccTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGc                                                                          <  1:733638/140‑1 (MQ=255)
                                                     cTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCATCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCa                                                                         <  1:1352819/140‑1 (MQ=255)
                                                     cTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCa                                                                         <  2:1325926/140‑1 (MQ=255)
                                                      tttttAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCAt                                                                        >  1:785918/1‑140 (MQ=255)
                                                        tttAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCAtgt                                                                      <  1:275424/140‑1 (MQ=255)
                                                            aCCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTa                                                                  <  1:282739/140‑1 (MQ=255)
                                                            aCCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTa                                                                  <  2:1079224/140‑1 (MQ=255)
                                                            aCCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTa                                                                  <  1:237179/140‑1 (MQ=255)
                                                            aCCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTa                                                                  <  2:514294/140‑1 (MQ=255)
                                                            aCCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTa                                                                  <  2:564594/140‑1 (MQ=255)
                                                                ccccACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGt                                                              >  1:1253001/1‑140 (MQ=255)
                                                                ccccACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGt                                                              >  2:1231888/1‑140 (MQ=255)
                                                                 cccACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTc                                                             >  2:589649/1‑140 (MQ=255)
                                                                 cccACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTc                                                             >  2:1090986/1‑140 (MQ=255)
                                                                       acTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGcc                                                       <  2:1253001/140‑1 (MQ=255)
                                                                           ttAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTggg                                                   >  2:1148793/1‑140 (MQ=255)
                                                                           ttAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTggg                                                   >  2:1362807/1‑140 (MQ=255)
                                                                            tAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGa                                                  <  2:79385/140‑1 (MQ=255)
                                                                              aTGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGAtt                                                >  1:639951/1‑140 (MQ=255)
                                                                               tGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTa                                               >  2:381872/1‑140 (MQ=255)
                                                                                gATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTAt                                              >  1:1438483/1‑140 (MQ=255)
                                                                                   cATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGt                                           >  2:1069293/1‑140 (MQ=255)
                                                                                   cATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGt                                           >  2:1083722/1‑140 (MQ=255)
                                                                                     ttGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTc                                         >  2:1424348/1‑140 (MQ=255)
                                                                                     ttGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTc                                         >  1:556350/1‑140 (MQ=255)
                                                                                         cGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTg                                     <  2:785918/140‑1 (MQ=255)
                                                                                         cGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTg                                     <  1:927205/140‑1 (MQ=255)
                                                                                             aTCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATgg                                 >  1:826387/1‑140 (MQ=255)
                                                                                                        tAAACTGTGTGCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTa                      <  2:1438483/140‑1 (MQ=255)
                                                                                                             tgtgtgCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGAAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACtt                 >  2:1296558/1‑140 (MQ=255)
                                                                                                               tgtgCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACtttt               >  1:397439/1‑140 (MQ=255)
                                                                                                               tgtgCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACtttt               >  2:152895/1‑140 (MQ=255)
                                                                                                               tgtgCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACtttt               >  2:1361118/1‑140 (MQ=255)
                                                                                                               tgtgCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACtttt               >  1:1026416/1‑140 (MQ=255)
                                                                                                               tgtgCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACtttt               >  1:557464/1‑140 (MQ=255)
                                                                                                               tgtgCCGTGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACtttt               >  1:497799/1‑140 (MQ=255)
                                                                                                                      tGCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGa        >  2:1357815/1‑140 (MQ=255)
                                                                                                                       gCTGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGCCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGAt       >  1:630414/1‑140 (MQ=255)
                                                                                                                         tGGCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTg     >  2:710317/1‑140 (MQ=255)
                                                                                                                           gCGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGAc   <  1:1231888/140‑1 (MQ=255)
                                                                                                                            cGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGAcc  <  2:279668/140‑1 (MQ=255)
                                                                                                                            cGAGTATCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGAcc  <  2:1026416/140‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                     
CTTTCCCTGTTCACACCTGCAGTGTGGGGCAACGGCTATAGCACCGTACAATCCTTTTTAACCGCCCCACCACTGTTAATGATCATTGCCGGGATCTTCCTTTGTAAACTGTGTGCCGTGCTGGCGAGTAGCGGTTCCGGCGCACCCGGTGGGGTCTTTACACCGACGCTATTTATCGGTCTTGCCATTGGCATGTTGTATGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACC  >  NC_000913/1666122‑1666385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑