Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,007,943	G→T	P50T (CCG→ACG)	yedD ←	lipoprotein YedD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,007,943	0	G	T	98.9%	304.0 / ‑3.1	88	P50T (CCG→ACG)	yedD	lipoprotein YedD
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (55/32);  total (56/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-01

CTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGGGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCGCCAGCAACAAGAGCGCGCCTGCAATTGCG  >  NC_000913/2007806‑2008079                                                                                                                                          |                                                                                                                                         cTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGc                                                                                                                                        >  2:389714/1‑140 (MQ=255) cTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGc                                                                                                                                        >  1:338890/1‑140 (MQ=255) cTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGc                                                                                                                                        >  1:393654/1‑140 (MQ=255) cTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGc                                                                                                                                        >  2:629338/1‑140 (MQ=255) cTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGc                                                                                                                                        >  1:619675/1‑140 (MQ=255) cTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCAGCACGGCAATCACCCTCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGc                                                                                                                                        >  2:1295946/1‑140 (MQ=255)     cGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGAc                                                                                                                                    >  1:1279156/1‑140 (MQ=255)          tACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCGGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAgcg                                                                                                                               <  2:806136/140‑1 (MQ=255)           aCGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAgcgc                                                                                                                              >  2:1218912/1‑140 (MQ=255)           aCGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAgcgc                                                                                                                              >  2:111731/1‑140 (MQ=255)            cGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCa                                                                                                                             <  1:762486/140‑1 (MQ=255)                aaCGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGtt                                                                                                                         >  2:1175162/1‑140 (MQ=255)                aaCGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGtt                                                                                                                         >  1:325346/1‑140 (MQ=255)                aaCGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGtt                                                                                                                         >  1:647178/1‑140 (MQ=255)                aaCGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGtt                                                                                                                         >  2:411045/1‑140 (MQ=255)                  cGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGc                                                                                                                       >  2:1314794/1‑140 (MQ=255)                   gTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCg                                                                                                                      <  2:684396/140‑1 (MQ=255)                       gTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGccc                                                                                                                  <  1:629338/140‑1 (MQ=255)                        tGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCt                                                                                                                 <  2:576072/140‑1 (MQ=255)                           gaTCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTg                                                                                                              >  1:289411/1‑140 (MQ=255)                           gaTCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTg                                                                                                              >  1:1225308/1‑140 (MQ=255)                            atcatcAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTgg                                                                                                             >  2:267153/1‑140 (MQ=255)                            atcatcAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTgg                                                                                                             >  2:73198/1‑140 (MQ=255)                            atcatcAGACATCAATGTCAACTTACCCGGCACGGCAATCACCAGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTgg                                                                                                             >  2:197499/1‑140 (MQ=255)                             tcatcaGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGTCGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGt                                                                                                            >  2:191947/1‑140 (MQ=255)                             tcatcaGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGTAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGt                                                                                                            >  1:1156088/1‑140 (MQ=255)                             tcatcaGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCa                                                                                                                             >  2:597808/1‑123 (MQ=255)                             tcatcaGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCa                                                                                                                             <  1:597808/123‑1 (MQ=255)                             tcatcaGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGt                                                                                                            >  1:787880/1‑140 (MQ=255)                                 caGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGc                                                                                                        >  1:619081/1‑140 (MQ=255)                                  aGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGcc                                                                                                       >  1:1390796/1‑140 (MQ=255)                                     cATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGt                                                                                                    >  1:608447/1‑140 (MQ=255)                                      aTCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTa                                                                                                   >  1:1455959/1‑140 (MQ=255)                                         aaTGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCGGTAGcc                                                                                                >  1:965089/1‑140 (MQ=255)                                         aaTGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGcc                                                                                                >  1:1385565/1‑140 (MQ=255)                                         aaTGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGcc                                                                                                >  1:789537/1‑140 (MQ=255)                                           tGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGTTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCg                                                                                              <  1:1182873/140‑1 (MQ=255)                                           tGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCg                                                                                              >  1:807541/1‑140 (MQ=255)                                                     cccGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTc                                                                                    >  1:209040/1‑140 (MQ=255)                                                     cccGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTc                                                                                    >  2:1473019/1‑140 (MQ=255)                                                       cggcacggcaATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCg                                                                                  >  2:48617/1‑140 (MQ=255)                                                         gcacggcaATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGgc                                                                                >  2:244425/1‑140 (MQ=255)                                                         gcacggcaATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGgc                                                                                >  2:854568/1‑140 (MQ=255)                                                          cacggcaATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGgcg                                                                               >  2:971219/1‑140 (MQ=255)                                                          cacggcaATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGgcg                                                                               >  1:60214/1‑140 (MQ=255)                                                             ggcaATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCgg                                                                            <  2:289411/140‑1 (MQ=255)                                                               caATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCgggg                                                                          >  1:1217408/1‑140 (MQ=255)                                                                    ccccGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGtttt                                                                     <  2:1106159/139‑1 (MQ=255)                                                                    aCCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGtttt                                                                     <  2:338890/140‑1 (MQ=255)                                                                       cGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCAc                                                                  <  2:619675/140‑1 (MQ=255)                                                                        gCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCGGTAGCCCGCCAGCCAGTCCGGCGC‑GGGGTTTTCACGa                                                                <  2:965089/140‑1 (MQ=255)                                                                         cTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGa                                                                <  1:854568/140‑1 (MQ=255)                                                                         cTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGa                                                                <  1:197499/140‑1 (MQ=255)                                                                         cTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGa                                                                <  1:48617/140‑1 (MQ=255)                                                                         cTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGa                                                                <  2:244761/140‑1 (MQ=255)                                                                         cTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGa                                                                <  1:481950/140‑1 (MQ=255)                                                                          tGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGAc                                                               <  1:1175162/140‑1 (MQ=255)                                                                          tGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGAAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGAc                                                               <  2:147447/140‑1 (MQ=255)                                                                           gCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACa                                                              <  1:389714/140‑1 (MQ=255)                                                                           gCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACa                                                              <  1:267153/140‑1 (MQ=255)                                                                           gCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACa                                                              <  1:1422882/140‑1 (MQ=255)                                                                           gCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACa                                                              <  1:678808/140‑1 (MQ=255)                                                                                 gACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTta                                                        <  1:73198/140‑1 (MQ=255)                                                                                    ggCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGTCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACTACATTGTTATAg                                                     >  2:1156522/1‑140 (MQ=255)                                                                                         tCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTc                                                <  1:411045/140‑1 (MQ=255)                                                                                              ggTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACtt                                           >  1:1264675/1‑140 (MQ=255)                                                                                                   cGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGca                                      >  1:1419715/1‑140 (MQ=255)                                                                                                   cGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGca                                      >  1:1379108/1‑140 (MQ=255)                                                                                                   cGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGca                                      >  1:243350/1‑140 (MQ=255)                                                                                                   cGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGca                                      <  1:244425/140‑1 (MQ=255)                                                                                                     cccTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGGTTTCACGACATTGTTATAGTTTTCGACTTCGGcaca                                    >  2:649030/1‑140 (MQ=255)                                                                                                      ccTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAg                                   <  1:1218912/140‑1 (MQ=255)                                                                                                          tttAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCg                               >  2:925210/1‑140 (MQ=255)                                                                                                          tttAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCg                               <  2:608447/140‑1 (MQ=255)                                                                                                            tAGTGACAATCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCGcc                             <  2:1217408/140‑1 (MQ=255)                                                                                                                    aTCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCGCCAGcaacaa                     >  1:148308/1‑140 (MQ=255)                                                                                                                    aTCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCGCCAGcaacaa                     >  2:475669/1‑140 (MQ=255)                                                                                                                    aTCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCGCCAGcaacaa                     >  1:863052/1‑140 (MQ=255)                                                                                                                    aTCAGGCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCGCCAGcaacaa                     >  2:119304/1‑140 (MQ=255)                                                                                                                    aTCAGGCTGCCTATCGCTTCCGGGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCGCCAGcaacaa                     >  1:1308807/1‑140 (MQ=255)                                                                                                                        ggCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCGCCAGCAACAAGAgc                 >  1:1360508/1‑140 (MQ=255)                                                                                                                        ggCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCGCCAGCAACAAGAgc                 >  2:577456/1‑140 (MQ=255)                                                                                                                         gCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCGCCAGCAACAAGAgcg                >  2:664626/1‑140 (MQ=255)                                                                                                                         gCTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCGCCAGCAACAAGAgcg                >  2:530279/1‑140 (MQ=255)                                                                                                                          cTGCCTATCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCGCCAGCAACAAGAgcgc               <  2:807541/140‑1 (MQ=255)                                                                                                                                 tCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCGCCAGCAACAAGAGCGCGCCTGCa        >  1:901909/1‑140 (MQ=255)                                                                                                                                 tCGCTTCCGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCGCCAGCAACAAGAGCGCGCCTGCa        <  1:475669/140‑1 (MQ=255)                                                                                                                                       ccGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCGCCAGCAACAAGAGCGCGCCTGCAATTGCg  <  1:417316/140‑1 (MQ=255)                                                                                                                                       ccGTGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACACCCCGCCAGCGACAAGAGCGCGCCCGCAATTGCg  >  1:714212/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         CTCACGTTTTACGGTAACGTTAGTGAGATCATCAGACATCAATGTCAACTTACCCGGCACGGCAATCACCCGCTGCCACTGACGGCAATCCAGGGTATCGCCCTCTTTAGTGACAATCAGGCTGCCTATCGCTTCCGGGCTGACCAGCGCACGTTGCGGCCCTTTGGTCTGCCAGTAGCCCGCCAGCCAGTCCGGCGCGGGGGTTTTCACGACATTGTTATAGTTTTCGACTTCGGCACAGCCCGCCAGCAACAAGAGCGCGCCTGCAATTGCG  >  NC_000913/2007806‑2008079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑