Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,174,403	C→A	C55F (TGC→TTC)	gatB ←	galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,174,403	0	C	A	100.0%	333.9 / NA	92	C55F (TGC→TTC)	gatB	galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (42/50);  total (42/50)

CTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCC  >  NC_000913/2174269‑2174539                                                                                                                                       |                                                                                                                                         cTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATc                                                                                                                                     >  1:200950/1‑140 (MQ=255) cTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATc                                                                                                                                     >  1:870513/1‑140 (MQ=255)    aaaaCATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCaaa                                                                                                                                  >  2:675654/1‑140 (MQ=255)      aaCATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATg                                                                                                                                <  1:1183580/140‑1 (MQ=255)        cATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGca                                                                                                                              <  2:831835/140‑1 (MQ=255)         aTAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGcac                                                                                                                             <  1:675654/140‑1 (MQ=255)         aTAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGcac                                                                                                                             <  2:317458/140‑1 (MQ=255)         aTAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGcac                                                                                                                             >  2:815164/1‑140 (MQ=255)         aTAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGcac                                                                                                                             <  1:106710/140‑1 (MQ=255)           aGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCacac                                                                                                                           <  2:870513/140‑1 (MQ=255)           aGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCacac                                                                                                                           <  1:89362/140‑1 (MQ=255)            ggTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATg                                                                                                                                <  1:500110/134‑1 (MQ=255)            ggTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATg                                                                                                                                >  2:500110/1‑134 (MQ=255)            ggTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACAcc                                                                                                                          <  1:796189/140‑1 (MQ=255)            ggTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACAcc                                                                                                                          <  1:330833/140‑1 (MQ=255)            ggTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACAcc                                                                                                                          >  2:1290796/1‑140 (MQ=255)            ggTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACAcc                                                                                                                          <  2:284756/140‑1 (MQ=255)            ggTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACAcc                                                                                                                          <  1:228354/140‑1 (MQ=255)             gTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACAcca                                                                                                                         <  2:603836/140‑1 (MQ=255)              tCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACAccat                                                                                                                        <  1:377385/140‑1 (MQ=255)                aCCCCTGTAAGATAGTCAGAATTTTATTTTGTAAAGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACAccatcc                                                                                                                      >  1:941759/1‑140 (MQ=255)                  cccTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACAccatccat                                                                                                                    >  2:51701/1‑140 (MQ=255)                   ccTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGca                                                                                                                              >  1:190869/1‑129 (MQ=255)                   ccTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGca                                                                                                                              <  2:190869/129‑1 (MQ=255)                   ccTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCata                                                                                                                   >  2:479925/1‑140 (MQ=255)                     tGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCaaa                                                                                                                                  <  2:500640/123‑1 (MQ=255)                     tGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCaaa                                                                                                                                  >  1:500640/1‑123 (MQ=255)                        aaGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGt                                                                                                              >  2:447719/1‑140 (MQ=255)                        aaGATAGTCAGAATTTTACTTTGTAATGCTTCGATGCCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCACAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGt                                                                                                              <  2:596613/140‑1 (MQ=255)                            tAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCt                                                                                                          >  1:1291134/1‑140 (MQ=255)                            tAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCt                                                                                                          >  1:854970/1‑140 (MQ=255)                             aGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTa                                                                                                         <  2:11786/140‑1 (MQ=255)                             aGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTa                                                                                                         <  2:729046/140‑1 (MQ=255)                              gTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTAt                                                                                                        <  2:967765/140‑1 (MQ=255)                              gTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTAt                                                                                                        <  1:732963/140‑1 (MQ=255)                              gTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTAt                                                                                                        <  2:242702/140‑1 (MQ=255)                               tCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCatat                                                                                                                  <  1:809826/129‑1 (MQ=255)                               tCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCatat                                                                                                                  >  2:809826/1‑129 (MQ=255)                               tCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTAtt                                                                                                       >  1:948899/1‑140 (MQ=255)                               tCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTAtt                                                                                                       >  2:1126124/1‑140 (MQ=255)                               tCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTAtt                                                                                                       >  2:1212687/1‑140 (MQ=255)                               tCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTAtt                                                                                                       >  1:1031185/1‑140 (MQ=255)                               tCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTAtt                                                                                                       >  1:1138198/1‑140 (MQ=255)                               tCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTAtt                                                                                                       >  1:878735/1‑140 (MQ=255)                               tCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTAtt                                                                                                       >  1:71715/1‑140 (MQ=255)                               tCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGCGAATATCAAATGCACACCATCCATATAGGTTTCTAtt                                                                                                       >  2:553570/1‑140 (MQ=255)                                  gAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCa                                                                                                    <  1:973860/140‑1 (MQ=255)                                   aaTTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCAt                                                                                                   <  1:1126124/140‑1 (MQ=255)                                   aaTTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCAt                                                                                                   <  1:1212687/140‑1 (MQ=255)                                   aaTTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCAt                                                                                                   <  2:1054267/140‑1 (MQ=255)                                   aaTTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCAt                                                                                                   <  1:51701/140‑1 (MQ=255)                                   aaTTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCAt                                                                                                   <  2:1244840/140‑1 (MQ=255)                                   aaTTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCAt                                                                                                   >  1:1074359/1‑140 (MQ=255)                                    attttattttGAAATGTTTCGAAACCAAAGCCAGAAATAAAAGGCATGCCTTGACCTACCGGAATTTCGCAAAACCTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCAtt                                                                                                  <  2:854970/140‑1 (MQ=255)                                     ttttattttGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTa                                                                                                 >  1:1080808/1‑140 (MQ=255)                                        tattttGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAAcc                                                                                              <  2:71715/140‑1 (MQ=255)                                        tattttGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAAcc                                                                                              <  2:1138198/140‑1 (MQ=255)                                        tattttGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTCCATTAAcc                                                                                              <  2:878735/140‑1 (MQ=255)                                              gTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGacac                                                                                        <  2:200950/140‑1 (MQ=255)                                              gTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGacac                                                                                        <  1:1255847/140‑1 (MQ=255)                                                       cgataccgatacCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTGTATTTCATTAACCCGACACTGGATTAAt                                                                               <  2:21841/140‑1 (MQ=255)                                                         ataccgataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTc                                                                             >  1:1164384/1‑140 (MQ=255)                                                           accgataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCaa                                                                           <  2:941759/140‑1 (MQ=255)                                                           accgataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCaa                                                                           <  1:1290796/140‑1 (MQ=255)                                                            ccgataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGNNACTGGATTAATTCAAc                                                                          <  1:479925/140‑1 (MQ=255)                                                              gataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAg                                                                        <  1:876387/140‑1 (MQ=255)                                                              gataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAg                                                                        <  1:75444/140‑1 (MQ=255)                                                               ataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAgg                                                                       <  1:1200277/140‑1 (MQ=255)                                                               ataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAgg                                                                       >  1:1013020/1‑140 (MQ=255)                                                               ataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAgg                                                                       <  2:198333/140‑1 (MQ=255)                                                               ataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAgg                                                                       >  2:1200277/1‑140 (MQ=255)                                                               ataccAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAgg                                                                       >  2:1043123/1‑140 (MQ=255)                                                                 accAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGaa                                                                     <  1:1262807/140‑1 (MQ=255)                                                                         tAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGa                                                             >  2:260450/1‑140 (MQ=255)                                                                          aaaaGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGAt                                                            <  1:447719/140‑1 (MQ=255)                                                                            aaGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTc                                                          <  2:948899/140‑1 (MQ=255)                                                                                cATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAg                                                                        >  2:458412/1‑122 (MQ=255)                                                                                cATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAg                                                                        <  1:458412/122‑1 (MQ=255)                                                                                cATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGac                                                      >  1:1142157/1‑140 (MQ=255)                                                                                 aTGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGaca                                                     >  1:960176/1‑140 (MQ=255)                                                                                  tGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGacac                                                    >  2:565651/1‑140 (MQ=255)                                                                                   gCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGAcaca                                                   >  1:1173716/1‑140 (MQ=255)                                                                                         aaCTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCtt                                             >  2:317300/1‑140 (MQ=255)                                                                                              aCGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAAtt                                        >  2:742537/1‑140 (MQ=255)                                                                                                   atatCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATttcttc                                   >  2:334677/1‑140 (MQ=255)                                                                                                      tCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTccgc                                <  1:334677/140‑1 (MQ=255)                                                                                                      tCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTccgc                                <  1:260450/140‑1 (MQ=255)                                                                                                      tCGCCAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTccgc                                >  2:1047258/1‑140 (MQ=255)                                                                                                         ccAAAACTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCcgccgc                             <  1:565651/140‑1 (MQ=255)                                                                                                            aaaCTACGATCCACTTTGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCAt                          >  1:585950/1‑140 (MQ=255)                                                                                                                          tttGGCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCaa            <  2:1031185/140‑1 (MQ=255)                                                                                                                             ggCAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACcg         >  1:89817/1‑140 (MQ=255)                                                                                                                               cAGTGGTGAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACcgcg       >  2:402549/1‑140 (MQ=255)                                                                                                                                    gtgAATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTcc  >  2:1315042/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                         CTGAAAACATAGGTCACCCCTGTAAGATAGTCAGAATTTTATTTTGTAATGCTTCGATACCGATACCAGAAATAAAAGGCATGCCGTGAACTAACGGAATATCGCCAAAACTACGATCCACTTTGGCAGTGGTGCATATCAAATGCACACCATCCATATAGGTTTCTATTTCATTAACCCGACACTGGATTAATTCAACAGGAATATTATGATTCTGACACAACTCTTTAATTTCTTCCGCCGCCATCGTAGAGGTCGCAACCGCGCCTCC  >  NC_000913/2174269‑2174539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑