Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,461,832	C→A	A176D (GCT→GAT)	fadL →	long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,461,832	0	C	A	100.0%	246.3 / NA	70	A176D (GCT→GAT)	fadL	long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (41/29);  total (41/29)

ACAGAGTTTAACGATACTTATGCAGGCGGCTCTGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGCTCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCTACCGCCAACGGTATTGACAGT  >  NC_000913/2461696‑2461959                                                                                                                                         |                                                                                                                                aCAGAGTTTAACGATACTTATGCAGGCGGCTCTGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATcg                                                                                                                              >  2:271002/1‑140 (MQ=255)    gagTTTAACGATACTTATGCAGGCGGCTCTGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATcgcgc                                                                                                                           <  2:335433/140‑1 (MQ=255)      gTTTAACGATACTTATGCAGGCGGCTCTGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGa                                                                                                                         >  2:1068539/1‑140 (MQ=255)               tACTTATGCAGGCGGCTCTGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAAc                                                                                                                >  2:335592/1‑140 (MQ=255)                 cTTATGCAGGCGGCTCTGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGt                                                                                                              >  2:366358/1‑140 (MQ=255)                 cTTATGCAGGCGGCTCTGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGt                                                                                                              >  1:20890/1‑140 (MQ=255)                    aTGCAGGCGGCTCTGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTc                                                                                                           <  2:936957/140‑1 (MQ=255)                         ggcggcTCTGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAgg                                                                                                      >  2:499798/1‑140 (MQ=255)                         ggcggcTCTGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAgg                                                                                                      <  2:451001/140‑1 (MQ=255)                          gcggcTCTGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGc                                                                                                     >  1:700470/1‑140 (MQ=255)                          gcggcTCTGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGc                                                                                                     >  1:624430/1‑140 (MQ=255)                            ggcTCTGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGa                                                                                                   >  2:1131333/1‑140 (MQ=255)                            ggcTCTGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGa                                                                                                   >  2:59168/1‑140 (MQ=255)                            ggcTCTGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGa                                                                                                   >  2:170534/1‑140 (MQ=255)                                ctGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTg                                                                                               <  1:1068539/140‑1 (MQ=255)                                ctGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTg                                                                                               <  1:364262/140‑1 (MQ=255)                                             aaCCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAgttggttg                                                                                  <  2:20890/140‑1 (MQ=255)                                             aaCCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAgttggttg                                                                                  >  2:36204/1‑140 (MQ=255)                                                  aCCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGc                                                                             >  1:522586/1‑140 (MQ=255)                                                  aCCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGc                                                                             >  2:38470/1‑140 (MQ=255)                                                  aCCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGc                                                                             >  2:319986/1‑140 (MQ=255)                                                  aCCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGc                                                                             >  1:628001/1‑140 (MQ=255)                                                              tGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCaa                                                                 >  2:827828/1‑140 (MQ=255)                                                              tGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCaa                                                                 >  2:218005/1‑140 (MQ=255)                                                               gAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAAt                                                                <  1:450361/140‑1 (MQ=255)                                                                      aaCTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCctgc                                                         >  2:1221400/1‑140 (MQ=255)                                                                      aaCTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCctgc                                                         <  1:499798/140‑1 (MQ=255)                                                                         ttAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTgg                                                      <  1:170534/140‑1 (MQ=255)                                                                          tAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGc                                                     <  1:1221400/140‑1 (MQ=255)                                                                             gCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGcc                                                    <  2:25714/138‑1 (MQ=255)                                                                             gCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGcc                                                    >  1:25714/1‑138 (MQ=255)                                                                             gCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGAGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCaa                                                  >  1:1051687/1‑140 (MQ=255)                                                                              cGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCaaa                                                 <  1:549549/140‑1 (MQ=255)                                                                              cGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCaaa                                                 <  2:624430/140‑1 (MQ=255)                                                                                gTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACt                                               >  1:411297/1‑140 (MQ=255)                                                                                gTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACt                                               >  1:1152549/1‑140 (MQ=255)                                                                                gTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACt                                               >  1:443555/1‑140 (MQ=255)                                                                                 tGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTc                                              >  2:1301965/1‑140 (MQ=255)                                                                                   cGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAg                                            >  2:497975/1‑140 (MQ=255)                                                                                   cGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAg                                            >  1:1188858/1‑140 (MQ=255)                                                                                   cGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAg                                            >  2:705066/1‑140 (MQ=255)                                                                                     tATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCa                                          >  1:107845/1‑140 (MQ=255)                                                                                     tATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCa                                          <  1:827828/140‑1 (MQ=255)                                                                                        cGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAgg                                       >  2:595928/1‑140 (MQ=255)                                                                                          cTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGc                                     <  1:319986/140‑1 (MQ=255)                                                                                            tAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCaa                                   >  1:659832/1‑140 (MQ=255)                                                                                             aaaTAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGc                                           <  2:361260/131‑1 (MQ=255)                                                                                             aaaTAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGc                                           >  1:361260/1‑131 (MQ=255)                                                                                                taatGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCAt                               <  1:38470/140‑1 (MQ=255)                                                                                                  atGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTg                             >  2:1237939/1‑140 (MQ=255)                                                                                                     cATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCa                          >  1:117096/1‑140 (MQ=255)                                                                                                        ggAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCt                       <  2:522586/140‑1 (MQ=255)                                                                                                        ggAGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCt                       >  2:439796/1‑140 (MQ=255)                                                                                                          aGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCTAc                     >  2:270231/1‑140 (MQ=255)                                                                                                          aGCTTTGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCTAc                     >  2:928492/1‑140 (MQ=255)                                                                                                             tttGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCTACCGc                  >  1:524022/1‑140 (MQ=255)                                                                                                              ttGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCTACCGcc                 >  1:473930/1‑140 (MQ=255)                                                                                                               tGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAGTCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCTACCGCCa                <  1:705066/140‑1 (MQ=255)                                                                                                               tGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCTACCGCCa                >  1:1234748/1‑140 (MQ=255)                                                                                                               tGGTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCTACCGCCa                <  2:628001/140‑1 (MQ=255)                                                                                                               tGGTCTTGGTTTCAACGCCGTCTACGATCGAGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCTACCGCCa                <  2:1051687/140‑1 (MQ=255)                                                                                                                 gTCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCTACCGCCAAc              <  2:659832/140‑1 (MQ=255)                                                                                                                  tCTTGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCTACCGCCAACg             <  1:1237939/140‑1 (MQ=255)                                                                                                                     tGGTTTCAACGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGGCAAACTCAGCAAGGGCAAGCATTGGCAGCTACCGCCAACGGTa          <  1:250547/140‑1 (MQ=255)                                                                                                                            aaCGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGTCAAACTCAGCAAGGGCAAGCATTGGCAGCTACCGCCAACGGTATTGACAg   >  2:136492/1‑140 (MQ=255)                                                                                                                            aaCGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCTACCGCCAACGGTATTGACAg   <  1:218005/140‑1 (MQ=255)                                                                                                                             aCGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCTACCGCCAACGGTATTGACAGt  <  2:443555/140‑1 (MQ=255)                                                                                                                             aCGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCTACCGCCAACGGTATTGACAGt  <  2:411297/140‑1 (MQ=255)                                                                                                                             aCGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCTACCGCCAACGGTATTGACAGt  <  2:1152549/140‑1 (MQ=255)                                                                                                                             aCGCCGTCTACGATCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCTACCGCCAACGGTATTGACAGt  <  1:254478/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                ACAGAGTTTAACGATACTTATGCAGGCGGCTCTGTCGGGGGTACAACCGACCTTGAAACCATGAACCTGAACTTAAGCGGTGCGTATCGCTTAAATAATGCATGGAGCTTTGGTCTTGGTTTCAACGCCGTCTACGCTCGCGCGAAAATTGAACGTTTCGCAGGCGATCTGGGGCAGTTGGTTGCTGGCCAAATTATGCAATCTCCTGCTGGCCAAACTCAGCAAGGGCAAGCATTGGCAGCTACCGCCAACGGTATTGACAGT  >  NC_000913/2461696‑2461959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑