Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,710,092	G→T	L25I (CTT→ATT)	rseD ←	rpoE leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,710,092	0	G	T	100.0%	282.3 / NA	80	L25I (CTT→ATT)	rseD	rpoE leader peptide
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (33/47);  total (33/47)

TCTGATCTCCCTTCTGGACCCGTTCAACCAGGACCTGGTCCGTTAACTGCTCGCTCATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAAGGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAA  >  NC_000913/2709955‑2710228                                                                                                                                          |                                                                                                                                         tCTGATCTCCCTTCTGGACCCGTTCAACCAGGACCTGGTCCGTTAACTGCTCGCTCATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGTAAGCAAAtgg                                                                                                                                        >  2:516115/1‑140 (MQ=255)       ctcCCTTCTGGACCCGTTCAACCAGGACCTGGTCCGTTAACTGCTCGCTCATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTagag                                                                                                                                  >  2:390862/1‑140 (MQ=255)          ccTTCTGGACCCGTTCAACCAGGACCTGGTCCGTTAACTGCTCGCTCATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAgtgt                                                                                                                               >  2:443767/1‑140 (MQ=255)            ttCTGGACCCGTTCAACCAGGACCTGGTCCGTTAACTGCTCGCTCATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGtct                                                                                                                             <  1:431294/140‑1 (MQ=255)                       ttCAACCAGGACCTGGTCCGTTAACTGCTCGCTCATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTag                                                                                                                                    <  2:1036598/122‑1 (MQ=255)                       ttCAACCAGGACCTGGTCCGTTAACTGCTCGCTCATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTag                                                                                                                                    >  1:1036598/1‑122 (MQ=255)                                gACCTGGTCCGTTAACTGCTCGCTCATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTa                                                                                                         >  2:483664/1‑140 (MQ=255)                                gACCTGGTCCGTTAACTGCTCGCTCATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTa                                                                                                         >  2:707856/1‑140 (MQ=255)                                 aCCTGGTCCGTTAACTGCTCGCTCATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTaa                                                                                                        <  1:707856/140‑1 (MQ=255)                                     ggTCCGTTAACTGCTCGCTCATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAACAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCa                                                                                                    >  2:1114857/1‑140 (MQ=255)                                      gTCCGTTAACTGCTCGCTCATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCAt                                                                                                   >  1:899214/1‑140 (MQ=255)                                      gTCCGTTAACTGCTCGCTCATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCAt                                                                                                   >  2:740953/1‑140 (MQ=255)                                           ttAACTGCTCGCTCATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGtt                                                                                              >  1:1229026/1‑140 (MQ=255)                                                tGCTCGCTCATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTtttgt                                                                                         >  1:1342956/1‑140 (MQ=255)                                                tGCTCGCTCATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTtttgt                                                                                         <  1:445328/140‑1 (MQ=255)                                                 gctcgctcATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTtttgtt                                                                                        >  1:1312437/1‑140 (MQ=255)                                                    cgctcATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTttgtttgt                                                                                     >  1:279123/1‑140 (MQ=255)                                                    cgctcATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTttgtttgt                                                                                     >  1:489353/1‑140 (MQ=255)                                                     gctcATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTc                                                                                    <  1:744834/140‑1 (MQ=255)                                                     gctcATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTc                                                                                    <  2:1137980/140‑1 (MQ=255)                                                     gctcATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTc                                                                                    <  2:4629/140‑1 (MQ=255)                                                        cATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATg                                                                                 <  1:390862/140‑1 (MQ=255)                                                        cATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATg                                                                                 <  2:899214/140‑1 (MQ=255)                                                        cATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATg                                                                                 <  1:1071409/140‑1 (MQ=255)                                                          tCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCt                                                                               <  2:1176321/140‑1 (MQ=255)                                                            cGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGt                                                                             >  2:789840/1‑140 (MQ=255)                                                            cGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGt                                                                             >  1:632792/1‑140 (MQ=255)                                                             gAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTa                                                                            <  1:551946/140‑1 (MQ=255)                                                               ggTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGTAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGa                                                                          <  1:516115/140‑1 (MQ=255)                                                               ggTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGa                                                                          <  2:632792/140‑1 (MQ=255)                                                               ggTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGa                                                                          <  1:483664/140‑1 (MQ=255)                                                               ggTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGa                                                                          <  1:562611/140‑1 (MQ=255)                                                               ggTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAACAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGa                                                                          <  1:1114857/140‑1 (MQ=255)                                                               ggTAAAGTCTCCCAAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGa                                                                          <  1:308842/140‑1 (MQ=255)                                                                gTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGAc                                                                         <  1:802882/140‑1 (MQ=255)                                                                gTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGAc                                                                         <  1:1349407/140‑1 (MQ=255)                                                                gTAAAGCCTCCCAAAACAAAATTCCCACGCGCTATCGAACCGCCCCTCAATTAGCTGCAATTTGAGCAAGCAATTGGTTGGAGTGTTTCGTTTTTGTAAAGTTCCGTAACGCTTCTGTTTTTGTTTGTCATGCTGTAGAc                                                                         <  2:198280/140‑1 (MQ=255)                                                                 tAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTc                                                                                    >  2:406637/1‑128 (MQ=255)                                                                 tAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTc                                                                                    <  1:406637/128‑1 (MQ=255)                                                                 tAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACg                                                                        <  1:559774/140‑1 (MQ=255)                                                                 tAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACg                                                                        <  2:1213710/140‑1 (MQ=255)                                                                   aaGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGa                                                                      <  2:23015/140‑1 (MQ=255)                                                                   aaGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGa                                                                      >  2:136909/1‑140 (MQ=255)                                                                   aaGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGa                                                                      >  1:281599/1‑140 (MQ=255)                                                                     gTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATc                                                                    >  2:44721/1‑140 (MQ=255)                                                                        tcCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCAtt                                                                 >  1:259722/1‑140 (MQ=255)                                                                         ccccAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATg                                                                                 >  1:1211302/1‑123 (MQ=255)                                                                         ccccAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATg                                                                                 <  2:1211302/123‑1 (MQ=255)                                                                          cccAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATtat                                                               <  2:489353/140‑1 (MQ=255)                                                                          cccAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATtat                                                               >  1:825050/1‑140 (MQ=255)                                                                             aaaccaaaTTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATtatcta                                                            <  2:1229026/140‑1 (MQ=255)                                                                                   aaTTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATta                                                      >  1:850687/1‑140 (MQ=255)                                                                                    aTTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATtat                                                     >  1:147721/1‑140 (MQ=255)                                                                                       tCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAg                                                  <  2:279123/140‑1 (MQ=255)                                                                                         cACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTc                                                >  1:994562/1‑140 (MQ=255)                                                                                           cgcgCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTa                                              >  2:377955/1‑140 (MQ=255)                                                                                            gcgcTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTAc                                             <  1:136909/140‑1 (MQ=255)                                                                                            gcgcTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTAc                                             <  1:541103/140‑1 (MQ=255)                                                                                                      aaCGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGaa                                   <  2:994562/140‑1 (MQ=255)                                                                                                       aCGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAAc                                  >  2:503288/1‑140 (MQ=255)                                                                                                       aCGCCACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAAc                                  <  1:1132262/140‑1 (MQ=255)                                                                                                          ccACTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATc                               >  1:1078126/1‑140 (MQ=255)                                                                                                            aCTCCATTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGc                             >  2:688467/1‑140 (MQ=255)                                                                                                                 aTTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTAt                        <  1:1152229/140‑1 (MQ=255)                                                                                                                 aTTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTAt                        <  2:147721/140‑1 (MQ=255)                                                                                                                 aTTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTAt                        <  1:688467/140‑1 (MQ=255)                                                                                                                 aTTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTAt                        <  1:44721/140‑1 (MQ=255)                                                                                                                 aTTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTAt                        <  2:1359568/140‑1 (MQ=255)                                                                                                                 aTTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTAt                        <  1:789840/140‑1 (MQ=255)                                                                                                                 aTTAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTAt                        <  1:296402/140‑1 (MQ=255)                                                                                                                   tAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCt                      >  2:439264/1‑140 (MQ=255)                                                                                                                   tAGCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCt                      <  1:443767/140‑1 (MQ=255)                                                                                                                     gCTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCtgt                    >  2:1201206/1‑140 (MQ=255)                                                                                                                      cTGCAATTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCtgtg                   <  2:1312437/140‑1 (MQ=255)                                                                                                                          aaTTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGa               >  1:962966/1‑140 (MQ=255)                                                                                                                          aaTTTGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGa               >  1:425029/1‑140 (MQ=255)                                                                                                                             ttGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAAt            <  1:377955/140‑1 (MQ=255)                                                                                                                             ttGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAAt            <  1:248842/140‑1 (MQ=255)                                                                                                                             ttGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAAt            <  1:740953/140‑1 (MQ=255)                                                                                                                             ttGAGCAAGCAAATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAAt            <  2:259722/140‑1 (MQ=255)                                                                                                                                      caaATGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAATGCCCATTTa   <  1:1201206/140‑1 (MQ=255)                                                                                                                                       aaaTGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAATGCCCATTTaa  <  2:962966/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         TCTGATCTCCCTTCTGGACCCGTTCAACCAGGACCTGGTCCGTTAACTGCTCGCTCATCCGAGGTAAAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAAGGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAA  >  NC_000913/2709955‑2710228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑