Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,164,458	C→A	L274M (CTG→ATG)	btuB →	cobalamin/cobinamide outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,164,458	0	C	A	100.0%	350.4 / NA	97	L274M (CTG→ATG)	btuB	cobalamin/cobinamide outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (49/48);  total (49/48)

AACTTTACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCC  >  NC_000913/4164323‑4164593                                                                                                                                         |                                                                                                                                        aaCTTTACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATgcg                                                                                                                                     <  1:1138306/140‑1 (MQ=255)     ttACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCtat                                                                                                                                 >  1:838085/1‑140 (MQ=255)           aTGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACg                                                                                                                             <  1:1161366/138‑1 (MQ=255)           aTGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACg                                                                                                                             >  2:1161366/1‑138 (MQ=255)           aTGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGc                                                                                                                           >  2:189221/1‑140 (MQ=255)           aTGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGc                                                                                                                           >  1:659227/1‑140 (MQ=255)           aTGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGc                                                                                                                           >  2:536039/1‑140 (MQ=255)           aTGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCG‑TGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCg                                                                                                                          >  1:357060/1‑140 (MQ=255)             gCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGa                                                                                                                         >  2:153202/1‑140 (MQ=255)             gCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCG‑TGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGaa                                                                                                                        <  2:357060/140‑1 (MQ=255)              ccTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATgcg                                                                                                                                     <  2:569023/127‑1 (MQ=255)              ccTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATgcg                                                                                                                                     >  1:569023/1‑127 (MQ=255)              ccTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATgcg                                                                                                                                     >  2:502630/1‑127 (MQ=255)              ccTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATgcg                                                                                                                                     <  1:502630/127‑1 (MQ=255)              ccTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGaa                                                                                                                        <  1:302653/140‑1 (MQ=255)              ccTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGaa                                                                                                                        <  1:54298/140‑1 (MQ=255)              ccTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGaa                                                                                                                        >  1:483786/1‑140 (MQ=255)              ccTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGaa                                                                                                                        <  2:364740/140‑1 (MQ=255)              ccTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGaa                                                                                                                        <  1:550996/140‑1 (MQ=255)                tGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACt                                                                                                                      >  1:545454/1‑140 (MQ=255)                     cGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTa                                                                                                                 >  1:617858/1‑140 (MQ=255)                       gCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGTGCTATAACGGCGAACTGATTaaa                                                                                                               >  2:1207645/1‑140 (MQ=255)                       gCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTaaa                                                                                                               >  1:716695/1‑140 (MQ=255)                           tgtgCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATcac                                                                                                           <  1:818904/140‑1 (MQ=255)                              gcgcGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAAc                                                                                                        <  1:536039/140‑1 (MQ=255)                               cgcgGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACt                                                                                                       <  2:838085/140‑1 (MQ=255)                                gcgGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTc                                                                                                      <  1:359372/140‑1 (MQ=255)                                 cgGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGTGCTATAACGGCGAACTGATTAAATCACAACTCa                                                                                                     <  1:1207645/140‑1 (MQ=255)                                  ggctatggctatGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCAt                                                                                                    >  2:1273776/1‑140 (MQ=255)                                   gctatggctatgATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCAtt                                                                                                   <  2:659227/140‑1 (MQ=255)                                    ctatggctatgATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTa                                                                                                  <  2:617858/140‑1 (MQ=255)                                     tatggctatgATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTAc                                                                                                 <  1:1118700/140‑1 (MQ=255)                                     tatggctatgATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTAc                                                                                                 <  1:730977/140‑1 (MQ=255)                                     tatggctatgATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTAc                                                                                                 <  1:907792/140‑1 (MQ=255)                                     tatggctatgATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTAc                                                                                                 <  1:1273776/140‑1 (MQ=255)                                     tatggctatgATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTAc                                                                                                 <  2:489154/140‑1 (MQ=255)                                      atggctatgATAACCCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTAc                                                                                                 <  1:886081/140‑1 (MQ=255)                                          ctatgATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATAACAACTCATTACCAGCt                                                                                            <  1:358079/140‑1 (MQ=255)                                            atgATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCtat                                                                                          <  1:525259/140‑1 (MQ=255)                                            atgATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCtat                                                                                          >  1:452828/1‑140 (MQ=255)                                              gATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAg                                                                                        >  2:177243/1‑140 (MQ=255)                                                tAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGcc                                                                                      <  2:936403/140‑1 (MQ=255)                                                  a‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCtat                                                                                          >  2:71291/1‑134 (MQ=255)                                                  a‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCtat                                                                                          <  1:71291/134‑1 (MQ=255)                                                    ccGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATa                                                                                   >  2:243120/1‑140 (MQ=255)                                                    ccGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATa                                                                                   >  1:667757/1‑140 (MQ=255)                                                     cGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAg                                                                                  >  1:851822/1‑140 (MQ=255)                                                       tACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCa                                                                                >  1:868577/1‑140 (MQ=255)                                                       tACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCa                                                                                >  1:515738/1‑140 (MQ=255)                                                       tACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCa                                                                                <  2:452828/140‑1 (MQ=255)                                                       tACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCa                                                                                <  1:372348/140‑1 (MQ=255)                                                        aCCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCaa                                                                               >  2:946535/1‑140 (MQ=255)                                                        aCCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCaa                                                                               >  2:139243/1‑140 (MQ=255)                                                         ccAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCaaa                                                                              <  2:856038/140‑1 (MQ=255)                                                         ccAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCaaa                                                                              >  2:775569/1‑140 (MQ=255)                                                            aTTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGAt                                                                           <  2:6282/140‑1 (MQ=255)                                                            aTTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGAt                                                                           <  1:153202/140‑1 (MQ=255)                                                            aTTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGAt                                                                           >  2:344309/1‑140 (MQ=255)                                                               aTGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTAc                                                                        >  2:187649/1‑140 (MQ=255)                                                                   cgcgTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACt                                                                    <  2:868577/140‑1 (MQ=255)                                                                    gcgTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTa                                                                   >  1:169453/1‑140 (MQ=255)                                                                    gcgTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGCGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTa                                                                   >  1:581456/1‑140 (MQ=255)                                                                     cgTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTAc                                                                  >  1:1039534/1‑140 (MQ=255)                                                                      gTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACg                                                                 <  2:479563/140‑1 (MQ=255)                                                                        attattCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGAt                                                               <  2:716695/140‑1 (MQ=255)                                                                        attattCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGAt                                                               <  1:243120/140‑1 (MQ=255)                                                                                 ccGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTAt                                                      >  1:523407/1‑140 (MQ=255)                                                                                 ccGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTAt                                                      >  1:189797/1‑140 (MQ=255)                                                                                  cGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATg                                                     >  1:1225395/1‑140 (MQ=255)                                                                                    gTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGt                                                   >  2:1086725/1‑140 (MQ=255)                                                                                       cACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGACGt                                                >  1:437563/1‑140 (MQ=255)                                                                                            ttGCTCGATACCCGTATACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGa                                           <  2:1225395/140‑1 (MQ=255)                                                                                              gCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGAtt                                         >  2:1337312/1‑140 (MQ=255)                                                                                                    tACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCgg                                   >  1:786203/1‑140 (MQ=255)                                                                                                         gTAAACTCTATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACg                              <  2:851822/140‑1 (MQ=255)                                                                                                              ctctATAGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCga                         <  1:1337312/140‑1 (MQ=255)                                                                                                                 tataGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatga                      >  1:1160776/1‑140 (MQ=255)                                                                                                                 tataGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatga                      >  2:1247254/1‑140 (MQ=255)                                                                                                                 tataGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatga                      >  2:877256/1‑140 (MQ=255)                                                                                                                   taGCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatgaga                    <  2:363700/140‑1 (MQ=255)                                                                                                                   taGCCAAAGTTGCGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatgaga                    <  2:581456/140‑1 (MQ=255)                                                                                                                     gCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatgagatg                  <  2:545454/140‑1 (MQ=255)                                                                                                                     gCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatgagatg                  <  1:139243/140‑1 (MQ=255)                                                                                                                     gCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatgagatg                  <  2:523407/140‑1 (MQ=255)                                                                                                                     gCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatgagatg                  <  2:515738/140‑1 (MQ=255)                                                                                                                     gCCAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatgagatg                  <  1:877256/140‑1 (MQ=255)                                                                                                                      ccAAAGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCgatgagatga                 >  1:766686/1‑140 (MQ=255)                                                                                                                        aaaGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAg               >  1:926951/1‑140 (MQ=255)                                                                                                                        aaaGTTGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAg               >  1:220098/1‑140 (MQ=255)                                                                                                                            ttGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATCGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAAt           <  2:1294474/140‑1 (MQ=255)                                                                                                                             tGGGACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATa          <  1:1247254/140‑1 (MQ=255)                                                                                                                               ggACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATaca        >  2:287807/1‑140 (MQ=255)                                                                                                                               ggACGCCGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATaca        >  1:301158/1‑140 (MQ=255)                                                                                                                                    ccGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTc   >  1:329979/1‑140 (MQ=255)                                                                                                                                     cGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTcc  <  2:189797/140‑1 (MQ=255)                                                                                                                                     cGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTcc  >  1:1136892/1‑140 (MQ=255)                                                                                                                                     cGGGATGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTcc  >  1:155969/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        AACTTTACTGATGCCTGGAGCGGCTTTGTGCGCGGCTATGGCTATGATAA‑CCGTACCAATTATGACGCGTATTATTCTCCCGGTTCACCGTTGCTCGATACCCGTAAACTCTATAGCCAAAGTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCC  >  NC_000913/4164323‑4164593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑