Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	508,538	G→T	E107D (GAG→GAT)	ybaQ →	putative DNA‑binding transcriptional regulator YbaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	508,538	0	G	T	100.0%	171.5 / NA	50	E107D (GAG→GAT)	ybaQ	putative DNA‑binding transcriptional regulator YbaQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (26/24);  total (26/24)

TTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTT  >  NC_000913/508405‑508671                                                                                                                                      |                                                                                                                                      ttGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCa                                                                                                                                 >  2:950993/1‑140 (MQ=255) ttGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCa                                                                                                                                 >  2:1285235/1‑140 (MQ=255)        cacaGTCGATTTTTTGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAgg                                                                                                                          >  2:225807/1‑140 (MQ=255)         acaGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGt                                                                                                                         <  1:767565/140‑1 (MQ=255)           aGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTcg                                                                                                                       >  1:405018/1‑140 (MQ=255)             tCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTcgcg                                                                                                                     >  2:835569/1‑140 (MQ=255)              cGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGTACGCCGTGAAGATCGTGCAAAAAAGGTCGCGt                                                                                                                    >  1:295569/1‑140 (MQ=255)              cGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGt                                                                                                                    <  1:1285235/140‑1 (MQ=255)              cGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGt                                                                                                                    <  2:1006240/140‑1 (MQ=255)              cGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGt                                                                                                                    <  1:950993/140‑1 (MQ=255)              cGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGt                                                                                                                    >  2:516078/1‑140 (MQ=255)              cGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGt                                                                                                                    <  2:405018/140‑1 (MQ=255)                       gCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCaaa                                                                                                           <  2:1112611/140‑1 (MQ=255)                          aaaCCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACa                                                                                                        <  1:158287/140‑1 (MQ=255)                          aaaCCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACa                                                                                                        <  1:860102/140‑1 (MQ=255)                           aaCCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACAt                                                                                                       >  1:1260374/1‑140 (MQ=255)                                 cAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGTACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTc                                                                                                 <  2:295569/140‑1 (MQ=255)                                 cAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTc                                                                                                 <  2:375354/140‑1 (MQ=255)                                     cggcggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCAtct                                                                                             >  1:1095293/1‑140 (MQ=255)                                        cggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGt                                                                                          <  2:1260374/140‑1 (MQ=255)                                        cggTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGt                                                                                          >  2:239206/1‑140 (MQ=255)                                          gTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAg                                                                                        >  2:866260/1‑140 (MQ=255)                                           ttGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAgg                                                                                       >  1:774929/1‑140 (MQ=255)                                                cTTTTGGAAGTTTAAAACAACATGCGCACCCACGCAGAATTGGGAAGGGTTGGAAAAGGGGCTGAATATTTGGCACGCCGGGAAGATCGTGGAAAAAAGGTTGCGTAAGGGCAAAACATCGCTTCACCTCTTAAGCCggg                                                                                  >  2:351596/1‑139 (MQ=255)                                                  ttGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATa                                                                                >  2:382244/1‑140 (MQ=255)                                                      gAAGTGGAAAACAACATGTGCACCTAGGTAGAATTGGTACGGATTGAAACATTGGCGGAATATTTGGCACGCCGTGAAGATCGCGTAAAAAAGGTCGCGTAAGGCCAAAACATGCCGTCATCTCGTAGGCCGGATAAGGc                                                                            <  2:669907/140‑1 (MQ=255)                                                         gTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGtt                                                                         <  1:702454/140‑1 (MQ=255)                                                                acaacaTGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcgccg                                                                  <  1:382244/140‑1 (MQ=255)                                                                    caTGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatc                                                              >  1:32957/1‑140 (MQ=255)                                                                       gcgcACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccgg                                                           <  2:32957/140‑1 (MQ=255)                                                                          cACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcat                                                        <  1:225807/140‑1 (MQ=255)                                                                           aCCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAAGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatc                                                       >  1:977446/1‑140 (MQ=255)                                                                             ccAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTGGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatccg                                                     <  2:977446/140‑1 (MQ=255)                                                                                       ttGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTg                                           >  1:546095/1‑140 (MQ=255)                                                                                         gggACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAg                                                   <  2:626936/130‑1 (MQ=255)                                                                                         gggACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAg                                                   >  1:626936/1‑130 (MQ=255)                                                                                               gATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGc                                   <  1:856329/140‑1 (MQ=255)                                                                                                 ttGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGa                                 <  2:774929/140‑1 (MQ=255)                                                                                                    aaaCAGTGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGc                              >  1:499551/1‑140 (MQ=255)                                                                                                          tGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGTCTGATGCGACGCTTAccc                        >  2:528859/1‑140 (MQ=255)                                                                                                          tGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCt                             <  1:713744/135‑1 (MQ=255)                                                                                                          tGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCt                             >  2:713744/1‑135 (MQ=255)                                                                                                          tGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTa                           <  1:1244219/137‑1 (MQ=255)                                                                                                          tGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTa                           >  2:1244219/1‑137 (MQ=255)                                                                                                          tGGCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTAccc                        >  2:1180262/1‑140 (MQ=255)                                                                                                            gCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGt                      >  2:282363/1‑140 (MQ=255)                                                                                                            gCTGAATATTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGt                      >  1:1155431/1‑140 (MQ=255)                                                                                                                   atTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCa               <  2:407316/140‑1 (MQ=255)                                                                                                                   atTTGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCa               <  1:282363/140‑1 (MQ=255)                                                                                                                    tttGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAg              <  2:260865/140‑1 (MQ=255)                                                                                                                    tttGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAg              <  1:866260/140‑1 (MQ=255)                                                                                                                      tGGCACGCCGTGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGc            >  1:592202/1‑140 (MQ=255)                                                                                                                                tGAAGATCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACtt  >  2:175286/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                      TTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCGCACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTT  >  NC_000913/508405‑508671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑