Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,101,514	0	G	T	24.4%	215.0 / 22.9	78	intergenic (+84/‑176)	sodA/kdgT	superoxide dismutase (Mn)/2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (7/52);  new base T (19/0);  total (26/52)
Fisher's exact test for biased strand distribution p-value = 9.80e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACG  >  NC_000913/4101375‑4101653                                                                                                                                             |                                                                                                                                            tCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTtg                                                                                                                                             <  2:886851/140‑1 (MQ=255)  cTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTtgt                                                                                                                                            >  1:942573/1‑140 (MQ=255)     gAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGAt                                                                                                                                         <  2:531516/140‑1 (MQ=255)      aaCGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATc                                                                                                                                        <  2:985734/140‑1 (MQ=255)          tggtgAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAAtt                                                                                                                                    >  1:296938/1‑140 (MQ=255)           ggtgAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAAttt                                                                                                                                   >  2:571641/1‑140 (MQ=255)            gtgAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGTGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTc                                                                                                                                  <  1:1264144/140‑1 (MQ=255)            gtgAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTc                                                                                                                                  <  2:1068550/140‑1 (MQ=255)               aaCTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TTGGTTTTTTGTTGTTAATTTCtaa                                                                                                                               >  1:1389593/1‑140 (MQ=255)               aaCTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTTTGATCAAtttttaa                                                                                                                               >  2:1315491/1‑136 (MQ=255)               aaCTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCaaa                                                                                                                               <  2:79139/140‑1 (MQ=255)               aaCTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCaaa                                                                                                                               <  1:732649/140‑1 (MQ=255)               aaCTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTTaaa                                                                                                                               >  2:635819/1‑140 (MQ=255)               aaCTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTTaaa                                                                                                                               >  2:228295/1‑140 (MQ=255)               aaCTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑GGGTTTTTTTGTGGTTAAATTTaaa                                                                                                                               >  1:470789/1‑140 (MQ=255)               aaCTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑AGGGTTTTTTTTGGTTAATTTTaaa                                                                                                                               >  2:732649/1‑140 (MQ=255)                 cTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTTTGATCAATTTTAAAAt                                                                                                                             >  2:1085111/1‑140 (MQ=255)                    ggACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATaaa                                                                                                                          <  1:1204245/140‑1 (MQ=255)                    ggACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑GGGTTTTTTTGTGGTCAATTTCCAAATaaa                                                                                                                          >  1:668966/1‑140 (MQ=255)                        gAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACa                                                                                                                      <  2:1345619/140‑1 (MQ=255)                        gAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACa                                                                                                                      <  2:1774401/140‑1 (MQ=255)                         aaGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTGTGATCAATTTCAAAATaaaaaaaa                                                                                                                     >  1:330962/1‑140 (MQ=255)                          agcagcGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTGTGATCAATTTCAAAATAAAAACaaa                                                                                                                    >  1:1587816/1‑139 (MQ=255)                               cGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGGATGGTTTTTTT‑TGATCAATTTTTAAAAAAAAAAAAAAATcc                                                                                                               >  2:5431/1‑140 (MQ=255)                               cGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAAATTTAAAATAAAAAAAATGATcc                                                                                                               >  2:301559/1‑140 (MQ=255)                                  cACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGat                                                                                                            <  2:1769499/140‑1 (MQ=255)                                  cACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGat                                                                                                            <  1:635819/140‑1 (MQ=255)                                  cACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGat                                                                                                            <  1:765307/140‑1 (MQ=255)                                  cACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGat                                                                                                            <  1:1718139/140‑1 (MQ=255)                                  cACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGat                                                                                                            <  1:17113/140‑1 (MQ=255)                                             gcgAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACGATGATCCGATAAAAATAAAAc                                                                                                 <  1:269239/140‑1 (MQ=255)                                             gcgAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAAc                                                                                                 <  2:121777/140‑1 (MQ=255)                                             gcgAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAAATTTAAAAAAAAAAAAAAGATCCGataaaaaaaaaaa                                                                                                 >  1:415902/1‑139 (MQ=255)                                               gAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAg                                                                                               <  2:1803911/140‑1 (MQ=255)                                               gAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAg                                                                                               <  1:1028771/140‑1 (MQ=255)                                                  aaaaTAATCATTTGCCCCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGt                                                                                            <  2:1669734/140‑1 (MQ=255)                                                        atCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAAt                                                                                      <  1:376801/140‑1 (MQ=255)                                                           aTTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGt                                                                                            <  2:215454/131‑1 (MQ=255)                                                           aTTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGt                                                                                            >  1:215454/1‑131 (MQ=255)                                                            tttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAt                                                                                  <  1:1832676/140‑1 (MQ=255)                                                             ttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAtg                                                                                 <  2:330962/140‑1 (MQ=255)                                                             ttGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑CGGGGTTTTTTTGATAAATTTTAAAAAAAAAAAAAATCTCCGTCAAAAATAAAAAAACGGTTCaaattagg                                                                                 >  2:667302/1‑134 (MQ=255)                                                              tGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGTTCAAATTCAAAAAAAAAACAAAGATCCGAAAAAAATAAAACAGCGGTTCAATTTAtgg                                                                                >  2:1198351/1‑139 (MQ=255)                                                              tGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTTAAAATAAAAAAAATGATCCGCTAAAAATAAAAAAACGTTTCTATTGAtgt                                                                                >  2:1799171/1‑140 (MQ=255)                                                              tGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAAAAGCGGTTCAATTGAtgg                                                                                >  1:1570604/1‑139 (MQ=255)                                                              tGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑GGGGTTTTTTGTTGTCAATTTTAAAATAAAAACAATGATCCGATAAAAATAAAAAAACGTTTCAATTTAtgt                                                                                >  2:838638/1‑140 (MQ=255)                                                               gccgccTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGAtgtg                                                                               <  2:432123/140‑1 (MQ=255)                                                                ccgccTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTTAAAATAAAAAAAAAGATCCGATAAAAATAAAAAAGCGTTTCAATTGATGTgg                                                                              >  2:1596567/1‑140 (MQ=255)                                                                       ctgcAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGAt                                                                       <  2:744637/140‑1 (MQ=255)                                                                       ctgcAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGAt                                                                       <  2:478915/140‑1 (MQ=255)                                                                       ctgcAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGAt                                                                       <  2:1396016/140‑1 (MQ=255)                                                                               aGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTTTGATTAATTTTAAAATAAAAAAAATTATCCACAAAAAATAAAACAACGTTTCAATAGATGTGGGTTTTATCACTTTTa                                                               >  2:368137/1‑140 (MQ=255)                                                                               aGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTa                                                               <  1:368137/140‑1 (MQ=255)                                                                               aGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTa                                                               <  1:1005538/140‑1 (MQ=255)                                                                               aGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGGTCAATTTTAAAATAAAAACAATGATCCGCTAAAAATAAAACAGCGGTTCAATTGATTTGGTTTTGATCACctttt                                                               >  1:1758163/1‑139 (MQ=255)                                                                                  cGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTCAAAATAAAAAAAAAGATCCGATAAAAAAAAAACAGCGGTTCAATTGATGTGGTTTTGATCACTTTTAttt                                                            >  1:7091/1‑139 (MQ=255)                                                                                         gCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAaat                                                     <  1:1141670/140‑1 (MQ=255)                                                                                         gCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTTAAAAAAAAAAAAAAGATCCGATAAAAATAAAAAAACGTTTCAATTGATGTGGTTTTGTTCACTTTTTTTTATTAaat                                                     >  2:1299039/1‑140 (MQ=255)                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTTTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAaatga                                                   >  1:1630102/1‑140 (MQ=255)                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAaatga                                                   <  1:467502/140‑1 (MQ=255)                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGGTCAAATTCAAAATAAAAAAAAAGATACGATAAAAATAAAAAAACGTTTCAATTTTTGTGGTTTTGATTACTTTTTTTGCTTAaatga                                                   >  2:318533/1‑140 (MQ=255)                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTTAAAATAAAAAAAAAGATCCGATAAAAATAAAAAAGCGTTTCAATTGATGTGGGTTTGATTACTTTTATTTATTAaatga                                                   >  2:960789/1‑140 (MQ=255)                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAaatga                                                   >  1:1515893/1‑140 (MQ=255)                                                                                           cGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGGTTCAATTGATGTGGTTTTGATCACTTTTTTTTATTAaatga                                                   >  1:134843/1‑140 (MQ=255)                                                                                            gCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAAAAAAACAGCGTTTTAATTGATGTGGTTTTTATCACTTTTATTTATTAaatgaa                                                  >  2:1243589/1‑140 (MQ=255)                                                                                             cATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAaatgaat                                                 <  2:1094066/140‑1 (MQ=255)                                                                                             cATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATTAAATTCCAAAAAAAAAAAAAAATCCGAGAAAAAAAAAAAAGCGGTTTCATTGATGTGGTTTTGATCACTTTTATTTattaaattaaa                                                 >  2:294443/1‑139 (MQ=255)                                                                                                   aGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTTTGATTAATTTTAAAATAAAAAAAAAGATCCGATAAAAATAAAACAGCGTTTTAATTTATGTGGTTTTTATCACTTTTATTGATTAAATTAATATCTAt                                           >  2:467502/1‑140 (MQ=255)                                                                                                   aGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGATCAAATTTAAAATAAAAAAAAAAAATCGATAAAAATAAAACAACGTTTCAATTTTTGTGGTTTTTATTACTTTTTTTTTTTAAAATAAAGTCttt                                           >  2:451497/1‑138 (MQ=255)                                                                                                     cTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCt                                         <  2:1764051/140‑1 (MQ=255)                                                                                                        aaaaaaTGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCg                                      <  2:1630102/140‑1 (MQ=255)                                                                                                        aaaaaaTGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCg                                      <  1:1772036/140‑1 (MQ=255)                                                                                                        aaaaaaTGAACCATCGCCAACGGCGG‑TAGCGTTTTTTGGATCAATTTCAAAATAAAAACAACGATTCTAGAAAAAAAAAACAGCGTTTCACTTTATGTGGGTTTGTTCACTTTTTTTTTTTAAATTTATGTTTttcttct                                      >  2:156361/1‑139 (MQ=255)                                                                                                         aaaaaTGAACCATCGCCAACGGCGG‑TGGGTTTTTTTTGGTAAATTTTAAAATAAAAACAATGATCCGATAAAAATAAAAAAGCGGTTCAATTGATGTGGGTTTTATCACTTTTTTTGATTAAATGAATGTCTATTTTctt                                     >  1:1838647/1‑138 (MQ=255)                                                                                                         aaaaaTGAACCATCGCCAACGGCGG‑TGGGTTTTTTGTGATCAATTTCAAAATAAAAAAAAAGATCCGATAAAAATAAAACAACGTTTTAATTGATGTGGTTTTTATCACTTTTATTTATTAAATGAAAGTCTATCTTCGt                                     >  1:1033483/1‑140 (MQ=255)                                                                                                          aaaaTGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGtt                                    <  1:1243589/140‑1 (MQ=255)                                                                                                               gAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCAt                               <  1:719769/140‑1 (MQ=255)                                                                                                               gAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCAt                               <  1:1299039/140‑1 (MQ=255)                                                                                                                aaCCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATc                              <  2:296938/140‑1 (MQ=255)                                                                                                                aaCCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATc                              <  2:204968/140‑1 (MQ=255)                                                                                                                 aCCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGAT‑AAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCaa                            <  2:554552/140‑1 (MQ=255)                                                                                                                  ccATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCaa                            <  2:408175/140‑1 (MQ=255)                                                                                                                       gCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTg                       <  1:1652839/140‑1 (MQ=255)                                                                                                                          aaCGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATg                    <  1:451497/140‑1 (MQ=255)                                                                                                                          aaCGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATg                    <  2:7091/140‑1 (MQ=255)                                                                                                                          aaCGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATg                    <  1:838638/140‑1 (MQ=255)                                                                                                                            cggcgg‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCt                  <  2:857403/140‑1 (MQ=255)                                                                                                                               cgg‑TGGTTTTTTTTTGATCAATTTCAAAAAAAAAACAATGATCCGATAAAAATAAAAAAGCGTTTCAATTGATGTGGTTTTTATCACTTTTTTTGATTAAATAAAAGTCTATTTTCGTTTCCATCAACACTGATGCTccc               >  2:1331790/1‑139 (MQ=255)                                                                                                                               cgg‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCa               <  1:1198351/140‑1 (MQ=255)                                                                                                                                gg‑tggtTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCAt              <  1:1275598/140‑1 (MQ=255)                                                                                                                                   tggtTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCTTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTg            <  2:145667/140‑1 (MQ=255)                                                                                                                                   tggtTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTg            <  1:1085111/140‑1 (MQ=255)                                                                                                                                    ggtTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGa           <  1:228295/140‑1 (MQ=255)                                                                                                                                             gtgATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACg  >  2:314051/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                            TCTGGAACGTGGTGAACTGGGACGAAGCAGCGGCACGTTTTGCGGCGAAAAAATAATCATTTGCCGCCTGCTGCAATGAGGCGTATAGGCCGCATATCAGCTTAAAAAATGAACCATCGCCAACGGCGG‑TGGTTTTTTTGTGATCAATTTCAAAATAAAAACAATGATCCGATAAAAATAAAACAGCGTTTCAATTGATGTGGTTTTGATCACTTTTATTGATTAAATGAATGTCTATCTTCGTTTCCATCAACACTGATGCTCCATTGAGGAATTACG  >  NC_000913/4101375‑4101653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑