Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,116,645	G→T	L161L (CTC→CTA)	wcaL ←	putative colanic biosynthesis glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,116,645	0	G	T	100.0%	316.7 / NA	89	L161L (CTC→CTA)	wcaL	putative colanic biosynthesis glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (47/42);  total (47/42)

ACGCCCATGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTGAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAA  >  NC_000913/2116511‑2116779                                                                                                                                       |                                                                                                                                       aCGCCCATGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCAc                                                                                                                                   >  1:277846/1‑140 (MQ=255) aCGCCCATGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCAc                                                                                                                                   <  1:619045/140‑1 (MQ=255) aCGCCCATGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCAc                                                                                                                                   >  2:1489490/1‑140 (MQ=255)  cGCCCATGCGCGATACGGCGATTTTTTCCCTCGGGTAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTAGGGAGTGTAGTGGTTTAGCACt                                                                                                                                  >  2:570464/1‑140 (MQ=255)  cGCCCATGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACt                                                                                                                                  >  1:954081/1‑140 (MQ=255)    cccATGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTc                                                                                                                                >  2:1729772/1‑140 (MQ=255)      cATGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTccc                                                                                                                              >  2:361543/1‑140 (MQ=255)        tGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGa                                                                                                                            >  2:1378427/1‑140 (MQ=255)        tGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGa                                                                                                                            <  2:1063235/140‑1 (MQ=255)        tGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGa                                                                                                                            >  2:1067640/1‑140 (MQ=255)        tGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGa                                                                                                                            <  2:1468493/140‑1 (MQ=255)        tGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGa                                                                                                                            >  2:1583627/1‑140 (MQ=255)        tGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGa                                                                                                                            <  2:37930/140‑1 (MQ=255)        tGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGa                                                                                                                            >  2:410626/1‑140 (MQ=255)        tGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGa                                                                                                                            >  2:455979/1‑140 (MQ=255)            cgATACGGCGATTTTTTCCCTCGGGCAGCCCA‑TTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGga                                                                                                                       <  1:1620341/140‑1 (MQ=255)             gATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGga                                                                                                                       <  2:933541/140‑1 (MQ=255)             gATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGga                                                                                                                       <  2:1317255/140‑1 (MQ=255)             gATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGga                                                                                                                       <  1:815169/140‑1 (MQ=255)             gATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGga                                                                                                                       <  1:455979/140‑1 (MQ=255)             gATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGga                                                                                                                       <  1:266490/140‑1 (MQ=255)             gATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGga                                                                                                                       <  1:1067640/140‑1 (MQ=255)              aTACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGgag                                                                                                                      <  1:713866/140‑1 (MQ=255)                     gATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCaa                                                                                                               <  1:1583627/140‑1 (MQ=255)                       ttttttCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATa                                                                                                             <  1:1729772/140‑1 (MQ=255)                       ttttttCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATa                                                                                                             <  1:1829221/140‑1 (MQ=255)                       ttttttCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATa                                                                                                             <  2:696020/140‑1 (MQ=255)                       ttttttCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATa                                                                                                             <  1:1331725/140‑1 (MQ=255)                       ttttttCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATa                                                                                                             <  2:1407049/140‑1 (MQ=255)                         ttttCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATAcc                                                                                                           >  2:546251/1‑140 (MQ=255)                                 cGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGa                                                                                                   >  1:831416/1‑140 (MQ=255)                                  gggCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGAt                                                                                                  >  2:1339248/1‑140 (MQ=255)                                                 tGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGcc                                                                                   <  2:1660727/140‑1 (MQ=255)                                                 tGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGcc                                                                                   <  1:1378427/140‑1 (MQ=255)                                                 tGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGcc                                                                                   >  2:274198/1‑140 (MQ=255)                                                  gCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCcg                                                                                  <  2:277846/140‑1 (MQ=255)                                                   cAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCcgc                                                                                 <  1:361543/140‑1 (MQ=255)                                                     gCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGa                                                                               <  2:1612436/140‑1 (MQ=255)                                                     gCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGa                                                                               >  1:668786/1‑140 (MQ=255)                                                      ccTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGaa                                                                              >  1:15033/1‑140 (MQ=255)                                                      ccTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGaa                                                                              >  1:1024837/1‑140 (MQ=255)                                                       cTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAAt                                                                             <  1:1489490/140‑1 (MQ=255)                                                       cTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAAt                                                                             <  1:1520255/140‑1 (MQ=255)                                                         tCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGa                                                                           <  2:1640105/140‑1 (MQ=255)                                                                  caGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGtt                                                                  >  1:1761385/1‑140 (MQ=255)                                                                  caGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGtt                                                                  >  2:168209/1‑140 (MQ=255)                                                                   aGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTc                                                                 >  1:164331/1‑140 (MQ=255)                                                                           tATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGtt                                                         >  1:1530784/1‑140 (MQ=255)                                                                            aTCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGttt                                                        >  2:699509/1‑140 (MQ=255)                                                                            aTCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGttt                                                        >  1:1656636/1‑140 (MQ=255)                                                                             tCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGtttt                                                       >  2:1521647/1‑140 (MQ=255)                                                                               ggTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTtgc                                                     >  1:1798664/1‑140 (MQ=255)                                                                               ggTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTtgc                                                     >  2:904489/1‑140 (MQ=255)                                                                               ggTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTtgc                                                     >  2:1432280/1‑140 (MQ=255)                                                                               ggTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTtgc                                                     >  1:1549339/1‑140 (MQ=255)                                                                                gTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTtgct                                                    <  2:929712/140‑1 (MQ=255)                                                                                 tAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTtgctg                                                   >  1:1578984/1‑140 (MQ=255)                                                                                 tAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTtgctg                                                   >  1:368789/1‑140 (MQ=255)                                                                                    cATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCgg                                                >  2:592502/1‑140 (MQ=255)                                                                                             gCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCcg                                       <  1:1179992/140‑1 (MQ=255)                                                                                             gCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCcg                                       <  1:1339248/140‑1 (MQ=255)                                                                                               cACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCcgcg                                     >  1:836849/1‑140 (MQ=255)                                                                                               cACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCcgcg                                     >  2:824290/1‑140 (MQ=255)                                                                                               cACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCcgcg                                     >  1:545066/1‑140 (MQ=255)                                                                                                aCGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCgg                                    <  2:471783/140‑1 (MQ=255)                                                                                                  ggCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGa                                  >  2:708854/1‑140 (MQ=255)                                                                                                   gCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGAc                                 <  1:498895/140‑1 (MQ=255)                                                                                                    cGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTTCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGAcc                                >  2:224624/1‑140 (MQ=255)                                                                                                    cGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGAcc                                >  2:462790/1‑140 (MQ=255)                                                                                                         cAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTGCCCCCGCGGGACCAAAAt                           <  1:570464/140‑1 (MQ=255)                                                                                                         cAGTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAAt                           <  1:708854/140‑1 (MQ=255)                                                                                                           gTTGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGa                         <  1:274198/140‑1 (MQ=255)                                                                                                            ttGCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAg                        >  1:499992/1‑140 (MQ=255)                                                                                                              gCTGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCg                      <  2:1578984/140‑1 (MQ=255)                                                                                                              gCTCATATTCGGGAGTGGAGGGGTTTAGCACTTCCCGTCTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGGCCAACATGAGCg                      >  2:1740174/1‑140 (MQ=255)                                                                                                                tGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGAt                    <  2:1798664/140‑1 (MQ=255)                                                                                                                tGATATTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGAt                    <  2:1549339/140‑1 (MQ=255)                                                                                                                   tatTCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGaa                 >  1:1482833/1‑140 (MQ=255)                                                                                                                      tCGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAacac              >  1:200773/1‑140 (MQ=255)                                                                                                                       cGGGAGTGTAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAAcaca             >  2:907519/1‑140 (MQ=255)                                                                                                                             tgtAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGgcg       <  2:1024837/140‑1 (MQ=255)                                                                                                                             tgtAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGgcg       <  1:904489/140‑1 (MQ=255)                                                                                                                               tAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGcgcg     >  1:236824/1‑140 (MQ=255)                                                                                                                               tAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGcgcg     >  2:1188789/1‑140 (MQ=255)                                                                                                                               tAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGcgcg     >  2:1330804/1‑140 (MQ=255)                                                                                                                               tAGTGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGcgcg     >  2:1646230/1‑140 (MQ=255)                                                                                                                                  tGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGaaa  <  1:168209/140‑1 (MQ=255)                                                                                                                                  tGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGaaa  <  1:1330804/140‑1 (MQ=255)                                                                                                                                  tGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGaaa  <  1:1521647/140‑1 (MQ=255)                                                                                                                                  tGGTTTAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGaaa  >  1:1367647/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       ACGCCCATGCGCGATACGGCGATTTTTTCCCTCGGGCAGCCCATTTTTTGCAGCCTTCCGGCCCACAGATCGCTTATCGGTAACATCAGGTCGCCACGGCGAAACAGTTGCTGATATTCGGGAGTGTAGTGGTTGAGCACTTCCCGACTGGAGATATCAATACCGTGGAAGATAGTGGCAATTTTGCCGCGAATGACACCCAGTTCGCGGAGTTTTGCTGCGGTTACCCCCGCGGGACCAAAATGAGCGATGAACACATCGGCGCGAAA  >  NC_000913/2116511‑2116779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑