Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,440,240	C→A	V264F (GTC→TTC)	rpoA ←	RNA polymerase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,440,240	0	C	A	100.0%	265.4 / NA	75	V264F (GTC→TTC)	rpoA	RNA polymerase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (44/31);  total (44/31)

GAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGA  >  NC_000913/3440104‑3440376                                                                                                                                         |                                                                                                                                         gAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGt                                                                                                                                       <  1:779853/140‑1 (MQ=255)   aGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCa                                                                                                                                     <  1:1425863/140‑1 (MQ=255)    gCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCaa                                                                                                                                    >  2:654747/1‑140 (MQ=255)    gCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCaa                                                                                                                                    >  2:431985/1‑140 (MQ=255)    gCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCaa                                                                                                                                    >  2:116528/1‑140 (MQ=255)     ccAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAAt                                                                                                                                   <  2:281018/140‑1 (MQ=255)        gCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTcc                                                                                                                                <  1:470606/140‑1 (MQ=255)             tCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATc                                                                                                                           >  2:920075/1‑140 (MQ=255)                    tctcAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACa                                                                                                                    >  2:787018/1‑140 (MQ=255)                       cAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAggg                                                                                                                 <  2:1096760/140‑1 (MQ=255)                        aGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGgc                                                                                                                >  2:794545/1‑140 (MQ=255)                        aGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGgc                                                                                                                >  1:1639675/1‑140 (MQ=255)                            agagATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCgcag                                                                                                            <  1:920075/140‑1 (MQ=255)                             gagaTTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCgcagc                                                                                                           >  2:1701539/1‑140 (MQ=255)                              agaTTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCgcagca                                                                                                          >  2:151313/1‑140 (MQ=255)                                 ttttttACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCCTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAgga                                                                                                       <  1:684923/140‑1 (MQ=255)                                   ttttACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAggatc                                                                                                     >  1:43183/1‑140 (MQ=255)                                      tACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAggatcgga                                                                                                  <  2:943820/140‑1 (MQ=255)                                            ggTTAGGCGTTTTAAGGAGCTCAACCTCG‑‑‑‑‑‑‑GTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGtt                                                                                     <  1:733507/140‑1 (MQ=255)                                             gTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAAct                                                                                           <  1:525174/140‑1 (MQ=255)                                                aGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTg                                                                                        >  2:661190/1‑140 (MQ=255)                                                   cGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGtt                                                                                     <  2:1639675/140‑1 (MQ=255)                                                      tttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTtct                                                                                  <  2:1790140/140‑1 (MQ=255)                                                      tttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTtct                                                                                  <  1:1253903/140‑1 (MQ=255)                                                       ttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAAc                                                                                            <  2:758139/129‑1 (MQ=255)                                                       ttAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAAc                                                                                            >  1:758139/1‑129 (MQ=255)                                                             aGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCttt                                                                           <  1:431985/140‑1 (MQ=255)                                                               cTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCa                                                                         >  2:982933/1‑140 (MQ=255)                                                               cTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCa                                                                         >  1:1760371/1‑140 (MQ=255)                                                               cTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCa                                                                         >  1:274895/1‑140 (MQ=255)                                                               cTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCa                                                                         >  1:285023/1‑140 (MQ=255)                                                                    ccTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCa                                                                    <  1:703717/140‑1 (MQ=255)                                                                      tCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAgg                                                                  <  2:1760371/140‑1 (MQ=255)                                                                      tCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAgg                                                                  <  2:274895/140‑1 (MQ=255)                                                                            cGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACg                                                            <  2:285023/140‑1 (MQ=255)                                                                              cTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTa                                                          >  2:202188/1‑140 (MQ=255)                                                                                   ccAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCa                                                     >  1:177224/1‑140 (MQ=255)                                                                                       aTCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTa                                                 >  1:1358322/1‑140 (MQ=255)                                                                                         cACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATAGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTAAGGCTGACGTACCTCACGTAAg                                               >  2:307959/1‑140 (MQ=255)                                                                                           ccGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTa                                                          >  1:952631/1‑127 (MQ=255)                                                                                           ccGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTa                                                          <  2:952631/127‑1 (MQ=255)                                                                                           ccGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTc                                             >  1:1383400/1‑140 (MQ=255)                                                                                           ccGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTc                                             >  2:1191593/1‑140 (MQ=255)                                                                                           ccGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTc                                             >  1:1627274/1‑140 (MQ=255)                                                                                           ccGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTc                                             >  2:1712136/1‑140 (MQ=255)                                                                                           ccGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTc                                             >  2:225947/1‑140 (MQ=255)                                                                                             gATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCaa                                           <  2:1306202/140‑1 (MQ=255)                                                                                                     ggATAGCTTCTGCTTTAAGGCAGTTAGCAGCGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCt                                   <  2:1737258/140‑1 (MQ=255)                                                                                                     ggATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCt                                   <  2:1625296/140‑1 (MQ=255)                                                                                                     ggATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGACAACGAAAGCt                                   <  1:1343508/140‑1 (MQ=255)                                                                                                      gATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCtt                                  >  2:703309/1‑140 (MQ=255)                                                                                                      gATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCtt                                  >  2:964577/1‑140 (MQ=255)                                                                                                      gATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCtt                                  >  1:1143272/1‑140 (MQ=255)                                                                                                         aGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCa                               >  1:349871/1‑140 (MQ=255)                                                                                                         aGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCa                               >  2:1462486/1‑140 (MQ=255)                                                                                                           cTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgt                             >  2:389709/1‑140 (MQ=255)                                                                                                           cTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgt                             >  2:664994/1‑140 (MQ=255)                                                                                                           cTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgt                             >  1:413/1‑140 (MQ=255)                                                                                                           cTTCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgt                             >  2:1128419/1‑140 (MQ=255)                                                                                                             tCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgttg                           <  1:202188/140‑1 (MQ=255)                                                                                                             tCTGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgttg                           <  1:664994/140‑1 (MQ=255)                                                                                                               tGCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAgttgtt                         <  1:116528/140‑1 (MQ=255)                                                                                                                gCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTc                        <  2:177224/140‑1 (MQ=255)                                                                                                                gCTTTAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTc                        <  1:654747/140‑1 (MQ=255)                                                                                                                    tAAGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGcc                    <  1:1385768/140‑1 (MQ=255)                                                                                                                     aaGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCa                   >  1:649530/1‑140 (MQ=255)                                                                                                                     aaGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCa                   >  1:593008/1‑140 (MQ=255)                                                                                                                     aaGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCa                   >  1:433997/1‑140 (MQ=255)                                                                                                                      aGGCAGTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAg                  >  1:468759/1‑140 (MQ=255)                                                                                                                           gTTAGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATgg             >  1:1272374/1‑140 (MQ=255)                                                                                                                              aGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTg          >  2:1826558/1‑140 (MQ=255)                                                                                                                              aGCAGAGCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACCCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTg          >  2:1427685/1‑140 (MQ=255)                                                                                                                                 agagCGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCg       <  1:964577/140‑1 (MQ=255)                                                                                                                                     cGGAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCacg   >  1:898071/1‑140 (MQ=255)                                                                                                                                      ggAAAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCacga  >  2:1145280/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         GAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATCGAACTCTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGA  >  NC_000913/3440104‑3440376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑