Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,824,794	C→A	L94M (CTG→ATG)	trmH →	tRNA (Gm18) 2'‑O‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,824,794	0	C	A	100.0%	241.2 / NA	69	L94M (CTG→ATG)	trmH	tRNA (Gm18) 2'‑O‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/41);  total (28/41)

TCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTCTGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTGGCGGATCAGGA  >  NC_000913/3824658‑3824921                                                                                                                                         |                                                                                                                                tCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACCCACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATgg                                                                                                                              <  2:619149/140‑1 (MQ=255)  cACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGc                                                                                                                             <  1:39201/140‑1 (MQ=255)  cACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGc                                                                                                                             <  2:1774961/140‑1 (MQ=255)  cACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGGACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAAGCCAGGGCATGCAGATTATGGc                                                                                                                             >  2:1583262/1‑140 (MQ=255)       cGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAAccc                                                                                                                        <  2:315462/140‑1 (MQ=255)          cTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATc                                                                                                                     <  1:419604/140‑1 (MQ=255)            ggCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCtt                                                                                                                   <  1:878461/140‑1 (MQ=255)              ccTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTc                                                                                                                 <  2:1299158/140‑1 (MQ=255)                 ggTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGa                                                                                                              >  1:967386/1‑140 (MQ=255)                 ggTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGGACAGGGGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGa                                                                                                              >  1:1650140/1‑140 (MQ=255)                  gTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGAt                                                                                                             <  1:1135574/140‑1 (MQ=255)                      ccGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACg                                                                                                         <  2:967386/140‑1 (MQ=255)                       cGCATGCGCACCATGGCTTCGGCAGCGGCGGGGAGTAACAGCTGGGGACAGGGGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCCTCTTTCTGATAACGc                                                                                                        >  1:592128/1‑140 (MQ=255)                            gcgcACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCg                                                                                                   >  1:1029490/1‑140 (MQ=255)                                      gCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGa                                                                                         <  1:99862/140‑1 (MQ=255)                                       cTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGaa                                                                                        <  1:1568711/140‑1 (MQ=255)                                       cTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGaa                                                                                        <  2:244792/140‑1 (MQ=255)                                        ttCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGaaa                                                                                       <  2:1340630/140‑1 (MQ=255)                                        ttCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGaaa                                                                                       <  2:1606138/140‑1 (MQ=255)                                        ttCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGaaa                                                                                       <  2:1029490/140‑1 (MQ=255)                                        ttCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGaaa                                                                                       <  1:1435406/140‑1 (MQ=255)                                          cGGCAGCGGCGGGGAGTAACAACCGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCAAGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAAtt                                                                                     >  2:967668/1‑140 (MQ=255)                                            gCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCCGGGCATGCAGATTATGGCAACCCATCTTTCTTATAACGCTGTCGATTTCCGCGAAATTGa                                                                                   >  2:217883/1‑140 (MQ=255)                                                cggcggGTAGTAACAGCTGGGGACAGGTGAAAACAAACCGCACCATTGGCGATGCCATCGCTCCTCTCAAAGGCCAGGGCATGCAGAATATGGCAAACCATCTTTCTGATAACGCTGTCGATTTCAGCCAAATTGATTaa                                                                               >  2:960985/1‑139 (MQ=255)                                                cggcggGGAGTAACAGCCGGGGACAGGGGAAAACACACCGCACCACTGGCGATGGCGTCGCTCATCTCAAAAGCCAGGGCATGCAGATTTTGGCAAACCCTCTTTTTGATAACGCTGTCGATTTCCGCGAAAATGGTTac                                                                               >  2:1103561/1‑140 (MQ=255)                                                 ggcggGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTaca                                                                              <  2:211121/140‑1 (MQ=255)                                                 ggcggGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTaca                                                                              <  2:570817/140‑1 (MQ=255)                                                 ggcggGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTaca                                                                              <  1:1556098/140‑1 (MQ=255)                                                      gtagtaACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGc                                                                         <  2:1586859/140‑1 (MQ=255)                                                      gtagtaACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGc                                                                         <  2:549447/140‑1 (MQ=255)                                                           aaCAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGAc                                                                    <  1:157520/140‑1 (MQ=255)                                                            aCAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGAcc                                                                   <  1:156030/140‑1 (MQ=255)                                                               gCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGc                                                                >  2:1516579/1‑140 (MQ=255)                                                                cTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCa                                                               >  2:218027/1‑140 (MQ=255)                                                                cTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCa                                                               >  2:804671/1‑140 (MQ=255)                                                                  gggTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCAtt                                                             >  1:1227516/1‑140 (MQ=255)                                                                  gggTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCAtt                                                             >  1:393225/1‑140 (MQ=255)                                                                   ggTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCAttt                                                            >  1:919888/1‑140 (MQ=255)                                                                    gTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCAtttt                                                           >  1:1737757/1‑140 (MQ=255)                                                                      aCAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGa                                                         <  1:1516579/140‑1 (MQ=255)                                                                          gTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATggg                                                     >  2:1107239/1‑140 (MQ=255)                                                                           tGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGa                                                    <  1:804671/140‑1 (MQ=255)                                                                           tGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGa                                                    <  2:899530/140‑1 (MQ=255)                                                                             aaaaCACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACa                                                  >  1:1007085/1‑140 (MQ=255)                                                                             aaaaCACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACa                                                  >  2:1118763/1‑140 (MQ=255)                                                                                    acCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGACCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGCACAGGAGaaa                                           >  2:1490491/1‑140 (MQ=255)                                                                                       gCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACg                                        >  1:588739/1‑140 (MQ=255)                                                                                          ccATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGc                                     <  2:1227516/140‑1 (MQ=255)                                                                                            aTTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAt                                   <  2:329226/140‑1 (MQ=255)                                                                                            aTTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCAt                                   <  2:588739/140‑1 (MQ=255)                                                                                                gCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACg                               >  1:1326607/1‑140 (MQ=255)                                                                                                gCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACg                               <  2:661370/140‑1 (MQ=255)                                                                                                   aTGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAg                            >  2:1232611/1‑140 (MQ=255)                                                                                                    tGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAgg                           <  2:919888/140‑1 (MQ=255)                                                                                                     gCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGa                          >  1:1513872/1‑140 (MQ=255)                                                                                                     gCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGa                          >  2:112629/1‑140 (MQ=255)                                                                                                     gCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGa                          >  1:624293/1‑140 (MQ=255)                                                                                                     gCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGa                          >  1:1747248/1‑140 (MQ=255)                                                                                                        gTCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGc                       >  2:55555/1‑140 (MQ=255)                                                                                                         tCGCTCATCTCAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGCAAACGGGCATCACGCAGGAAGCa                      >  1:148842/1‑140 (MQ=255)                                                                                                                   cAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCTCGAAATTGATTACACTCGCCCGTCCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTgg            <  2:1339840/140‑1 (MQ=255)                                                                                                                   cAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTgg            <  1:622965/140‑1 (MQ=255)                                                                                                                   cAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTgg            <  1:1583262/140‑1 (MQ=255)                                                                                                                   cAAAGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTgg            <  1:1103561/140‑1 (MQ=255)                                                                                                                     aaGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTGGCg          <  2:1777941/140‑1 (MQ=255)                                                                                                                      aGGCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTGGCgg         >  1:1592267/1‑140 (MQ=255)                                                                                                                        gCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTGGCGGAt       <  1:333137/140‑1 (MQ=255)                                                                                                                        gCCAGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTGGCGGAt       <  1:1815081/140‑1 (MQ=255)                                                                                                                           aGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTGGCgg         <  1:415662/135‑1 (MQ=255)                                                                                                                           aGGGCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTGGCgg         >  2:415662/1‑135 (MQ=255)                                                                                                                            gggCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTGGCGGATCAgg   >  2:746168/1‑140 (MQ=255)                                                                                                                             ggCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTGGCGGATCAGGa  <  1:1107239/140‑1 (MQ=255)                                                                                                                             ggCATGCAGATTATGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTGGCGGATCAGGa  <  1:1232611/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                TCACGCCGTCTGGCCTGGTAGCCGCATGCGCACCATGGCTTCGGCAGCGGCGGGTAGTAACAGCTGGGTACAGGTGAAAACACACCGCACCATTGGCGATGCCGTCGCTCATCTCAAAGGCCAGGGCATGCAGATTCTGGCAACCCATCTTTCTGATAACGCTGTCGATTTCCGCGAAATTGATTACACTCGCCCGACCTGCATTTTGATGGGACAGGAGAAAACGGGCATCACGCAGGAAGCATTGGCCCTGGCGGATCAGGA  >  NC_000913/3824658‑3824921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑