Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,335,6910TG22.7% 217.7 / 17.5 97intergenic (+24/‑140)ymiC/acnAprotein YmiC/aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base T (27/48);  new base G (22/0);  total (49/48)
Fisher's exact test for biased strand distribution p-value = 2.23e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 113 total aligned reads displayed.

GTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCT  >  NC_000913/1335556‑1335830
                                                                                                                                       |                                                                                                                                           
gTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTc                                                                                                                                         >  1:280829/1‑140 (MQ=255)
  tAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAt                                                                                                                                       >  2:333857/1‑140 (MQ=255)
  tAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCAt                                                                                                                                       >  2:145019/1‑140 (MQ=255)
        tgtAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgctt                                                                                                                                 <  2:333694/140‑1 (MQ=255)
        tgtAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgctt                                                                                                                                 <  1:1502602/140‑1 (MQ=255)
         gtAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTTACCCCGCTGCGGCGGGGTTGTCAtttgcttt                                                                                                                                >  1:1383687/1‑140 (MQ=255)
         gtAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgcttt                                                                                                                                >  1:808003/1‑140 (MQ=255)
         gtAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgcttt                                                                                                                                >  2:549972/1‑140 (MQ=255)
         gtAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCAtttgcttt                                                                                                                                >  2:1079090/1‑140 (MQ=255)
         gtAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCAtttgcttt                                                                                                                                >  2:260412/1‑140 (MQ=255)
          tAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgctttt                                                                                                                               >  1:1168990/1‑139 (MQ=255)
          tAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgctttg                                                                                                                               >  1:1124427/1‑140 (MQ=255)
              acaggATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCcac                                                                                                                           <  1:996949/136‑1 (MQ=255)
                aTGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACaa                                                                                                                         <  2:1287264/140‑1 (MQ=255)
                 tGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAg                                                                                                                        >  2:268527/1‑140 (MQ=255)
                 tGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTTATTTTCTTTGCCACAAg                                                                                                                        >  2:254265/1‑140 (MQ=255)
                 tGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTTCTTTTCCACAAg                                                                                                                        >  2:397505/1‑140 (MQ=255)
                      aaCACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCGTTTTGTTTTCCCCAAGGgttt                                                                                                                   >  2:1416962/1‑139 (MQ=255)
                       acacAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTTATTTTCCTTGCCACAAGGTTtct                                                                                                                  >  2:852420/1‑140 (MQ=255)
                       acacAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTtct                                                                                                                  <  1:1720820/140‑1 (MQ=255)
                       acacAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTtct                                                                                                                  <  1:1079090/140‑1 (MQ=255)
                       acacAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTtct                                                                                                                  <  2:1124427/140‑1 (MQ=255)
                       acacAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTtct                                                                                                                  >  1:568455/1‑140 (MQ=255)
                       acacAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGACGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTTCCACAAAGGTtct                                                                                                                  >  1:1019877/1‑140 (MQ=255)
                        cacaAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTtctc                                                                                                                 <  1:679681/140‑1 (MQ=255)
                           aaaTATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTctcctc                                                                                                              >  1:1104346/1‑140 (MQ=255)
                              tatGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACT‑ATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCtttt                                                                                                          <  1:567543/140‑1 (MQ=255)
                                tGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTa                                                                                                         <  2:1127365/140‑1 (MQ=255)
                                  aaaTATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCTAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGCGTTGTCTTTTTCTTTGCCACAAGGGTTTTCCTCTTTTAta                                                                                                       >  1:1369573/1‑139 (MQ=255)
                                  aaaTATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATc                                                                                                       >  1:1052696/1‑140 (MQ=255)
                                       ttGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAAttt                                                                                                  <  1:260412/140‑1 (MQ=255)
                                         ggTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCCTTTTCTTTGTCACAAGGTTTCTCCTCTTTTTTCAAttttg                                                                                                >  2:1353815/1‑138 (MQ=255)
                                         ggTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTTCTTTTCCCCAAGGTTTCTCCTCTTTTATCAAttttg                                                                                                >  2:1394358/1‑138 (MQ=255)
                                         ggTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTTCTTTGCCACAAGGTTTCTCCTCTTTTATCAAttttg                                                                                                >  2:1477938/1‑138 (MQ=255)
                                               ggATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCCCAAGGTTTTTCCCCTTTTTTCAATTTGGgttttt                                                                                          >  2:1599202/1‑140 (MQ=255)
                                               ggATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGgttgtt                                                                                          >  1:453485/1‑140 (MQ=255)
                                               ggATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGACGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCCCAAGGTTTCTCCTCTTTTTTCAATTTGGggtgtt                                                                                          >  2:941745/1‑140 (MQ=255)
                                                     gcgcGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCaaa                                                                                    >  1:127801/1‑140 (MQ=255)
                                                       gcgTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATc                                                                                  <  2:1122540/140‑1 (MQ=255)
                                                             tctcTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTAcg                                                                            <  2:925166/140‑1 (MQ=255)
                                                             tctcTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTAcg                                                                            <  1:1727042/140‑1 (MQ=255)
                                                             tctcTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTAcg                                                                            <  1:1477938/140‑1 (MQ=255)
                                                             tctcTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTAcg                                                                            <  1:1664379/140‑1 (MQ=255)
                                                              ctctGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTAcgc                                                                           <  1:524906/140‑1 (MQ=255)
                                                               tctGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTAcgcg                                                                          <  1:1416962/140‑1 (MQ=255)
                                                               tctGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTAcgcg                                                                          >  1:1710119/1‑140 (MQ=255)
                                                                   tCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGt                                                                      <  1:333857/140‑1 (MQ=255)
                                                                     gATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGttt                                                                    <  1:1029362/140‑1 (MQ=255)
                                                                        gCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgtg                                                                 <  1:549972/140‑1 (MQ=255)
                                                                          tcttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGtt                                                               <  2:645482/140‑1 (MQ=255)
                                                                          tcttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCCGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTAGCAATTTGATTTTTTACCAAATCGTTACGCGATGTTTGTGtt                                                               <  2:965963/140‑1 (MQ=255)
                                                                           cttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTa                                                              >  2:1093845/1‑140 (MQ=255)
                                                                            ttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTAt                                                             <  2:127801/140‑1 (MQ=255)
                                                                              ctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCt                                                           <  2:73743/140‑1 (MQ=255)
                                                                                gggCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCttt                                                         <  2:1402913/140‑1 (MQ=255)
                                                                                 ggCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTTCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCtttt                                                        >  2:534724/1‑139 (MQ=255)
                                                                                 ggCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTa                                                        >  1:698151/1‑140 (MQ=255)
                                                                                     gAACTCCTCTGGATCATTACTCACTGATCCTTTACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAatat                                                    <  2:1383687/140‑1 (MQ=255)
                                                                                      aaCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCt                                                           >  1:279212/1‑132 (MQ=255)
                                                                                      aaCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATAtt                                                   <  1:268527/140‑1 (MQ=255)
                                                                                      aaCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATAtt                                                   >  1:1581942/1‑140 (MQ=255)
                                                                                      aaCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATAtt                                                   >  2:834164/1‑140 (MQ=255)
                                                                                      aaCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATAtt                                                   <  2:568455/140‑1 (MQ=255)
                                                                                      aaCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATAtt                                                   >  2:32849/1‑140 (MQ=255)
                                                                                           ctctGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCAccc                                              <  2:1560991/140‑1 (MQ=255)
                                                                                           ctctGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCAccc                                              <  1:1487341/140‑1 (MQ=255)
                                                                                            tctGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCt                                             <  2:1656013/140‑1 (MQ=255)
                                                                                             ctGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTg                                            <  2:1104346/140‑1 (MQ=255)
                                                                                                gATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAag                                         <  1:254265/140‑1 (MQ=255)
                                                                                                     ttACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTTTCAATTTGGGGTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAAt                                    >  2:571409/1‑140 (MQ=255)
                                                                                                     ttACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTTACCCTGAAGAGAAt                                    >  2:746197/1‑140 (MQ=255)
                                                                                                     ttACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTATCATTTGCTTTGCCACAAAGTTTTTCCTCTTTTATTAATTTGGGTTGTTTTCAAATATTTAAGCGATGGTTGTGTTATTTTTAATATTTAACCTGAAGAGaaa                                    >  2:1679043/1‑139 (MQ=255)
                                                                                                        cTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAg                                 <  2:808003/140‑1 (MQ=255)
                                                                                                          cACTGATCCTTGACCCCGCTGCGGCGGGGTTTTCCTTTTCTTTGCCACAAGGTTTTTCCTCTTTTTTCAATTTGGGTTGTTATTAAATTGTTTAGCGGTGTTTGTGTTATTTTTTATTTTTACCCTGAAAAGAAtttggg                               >  2:1692728/1‑135 (MQ=255)
                                                                                                          cACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAggg                               <  1:1643759/140‑1 (MQ=255)
                                                                                                          cACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAggg                               >  2:1643759/1‑140 (MQ=255)
                                                                                                           aCTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGGTTCTCCTCTTTTATCAATTTGGGGTGTTATCAAATCCTTTCGCGATGTTTGTGTTATCTTTTATATTTACCCTGAAGAGAATCAGGGc                              >  2:119020/1‑140 (MQ=255)
                                                                                                             tGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTTCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGGTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTTACCCTGAAGAGAATCAGGGCtt                            >  1:1412492/1‑140 (MQ=255)
                                                                                                               aTCCTTGACCCCGCTGCGGCGGGGTTGTCATTTTCTTTGCCACAAGGTTTCTCCTCTTTTTTCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTTTGTTATCTTTTATAATCACCCTGTAGAGAATCAGGGCTTCg                          >  2:1719937/1‑140 (MQ=255)
                                                                                                                  cTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCaa                       <  2:570481/140‑1 (MQ=255)
                                                                                                                    tGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTTCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAcc                     >  2:694268/1‑140 (MQ=255)
                                                                                                                     gACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAccc                    >  1:936697/1‑140 (MQ=255)
                                                                                                                     gACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAccc                    <  1:32849/140‑1 (MQ=255)
                                                                                                                     gACCCCGCTGCGGCGGGGGTGTCATTTGCTTTTCCACAAGGGTTCTCCTCTTTTATCAATTTGGGGTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAccc                    >  2:1538791/1‑140 (MQ=255)
                                                                                                                      aCCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCt                   <  1:442298/140‑1 (MQ=255)
                                                                                                                        cccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGt                 <  1:670454/140‑1 (MQ=255)
                                                                                                                         ccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCa                        <  2:376164/132‑1 (MQ=255)
                                                                                                                         ccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCa                        >  1:376164/1‑132 (MQ=255)
                                                                                                                         ccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTc                >  1:198686/1‑140 (MQ=255)
                                                                                                                         ccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTc                >  2:1373007/1‑140 (MQ=255)
                                                                                                                         ccGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGGTTCTCATCTTTTTTCAATTTGGGGTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTc                >  2:278868/1‑140 (MQ=255)
                                                                                                                          cGCTGCGGCGGGGTTGTCAATTGCTTTTCCACAAGGTTTCTCCTCTTTTATCAATTTTGGCTGTTATCAAATCGTTATGCGATGTTTGTGTTATCTTTAATATTCACCCTCAAGAGAATCAGGGCTTTGCAACCCCGTcc               >  2:924452/1‑139 (MQ=255)
                                                                                                                           gCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCAt              <  1:656198/140‑1 (MQ=255)
                                                                                                                           gCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCAt              >  2:737221/1‑140 (MQ=255)
                                                                                                                           gCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCAt              >  1:284621/1‑140 (MQ=255)
                                                                                                                           gCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGGTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCAt              >  1:1654023/1‑140 (MQ=255)
                                                                                                                                ggcggGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAagg         <  1:707215/140‑1 (MQ=255)
                                                                                                                                  cggGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAaggag       <  2:1369573/140‑1 (MQ=255)
                                                                                                                                  cggGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAaggag       <  1:1719937/140‑1 (MQ=255)
                                                                                                                                       ttGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCt  <  2:284655/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                           
GTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCT  >  NC_000913/1335556‑1335830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑