Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,104,229	C→A	E249D (GAG→GAT)	cpxA ←	sensory histidine kinase CpxA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,104,229	0	C	A	100.0%	294.3 / NA	83	E249D (GAG→GAT)	cpxA	sensory histidine kinase CpxA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (40/43);  total (40/43)

GACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAG  >  NC_000913/4104092‑4104364                                                                                                                                          |                                                                                                                                        gACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATc                                                                                                                                       >  1:1037964/1‑140 (MQ=255)      cACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTgag                                                                                                                                  >  1:783524/1‑140 (MQ=255)      cACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTgag                                                                                                                                  <  2:1313900/140‑1 (MQ=255)       aCCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTgaga                                                                                                                                 <  1:965165/140‑1 (MQ=255)         cAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTgagaga                                                                                                                               <  1:554939/140‑1 (MQ=255)           aCAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGata                                                                                                                             >  2:1016734/1‑140 (MQ=255)            cAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGatat                                                                                                                            >  2:409095/1‑140 (MQ=255)              gATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCa                                                                                                                          >  1:804827/1‑140 (MQ=255)              gATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCa                                                                                                                          >  2:1509986/1‑140 (MQ=255)               aTCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAg                                                                                                                         >  2:722243/1‑140 (MQ=255)               aTCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAg                                                                                                                         <  2:1193591/140‑1 (MQ=255)               aTCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAg                                                                                                                         <  2:1581876/140‑1 (MQ=255)               aTCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAg                                                                                                                         <  1:1509986/140‑1 (MQ=255)                 cGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGaa                                                                                                                       >  2:473016/1‑140 (MQ=255)                   ttGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTgagaga                                                                                                                               <  1:810954/130‑1 (MQ=255)                   ttGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTgagaga                                                                                                                               >  2:810954/1‑130 (MQ=255)                   ttGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAg                                                                                                                     >  2:1577510/1‑140 (MQ=255)                    tGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGc                                                                                                                    <  1:1439563/140‑1 (MQ=255)                      aTCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAg                                                                                                                  >  1:1417388/1‑140 (MQ=255)                      aTCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAg                                                                                                                  >  1:1640606/1‑140 (MQ=255)                       tCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGa                                                                                                                 <  2:502514/140‑1 (MQ=255)                       tCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGa                                                                                                                 >  2:90479/1‑140 (MQ=255)                       tCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGa                                                                                                                 <  1:473016/140‑1 (MQ=255)                         aTGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACg                                                                                                               <  1:985407/140‑1 (MQ=255)                         aTGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACg                                                                                                               <  1:740012/140‑1 (MQ=255)                         aTGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACg                                                                                                               <  1:887003/140‑1 (MQ=255)                           gCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACgct                                                                                                             >  1:839226/1‑140 (MQ=255)                              gTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACgctgct                                                                                                          >  1:984408/1‑140 (MQ=255)                               tCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGctgctg                                                                                                         >  1:358284/1‑140 (MQ=255)                                    aCGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAgg                                                                                                    >  2:1281903/1‑140 (MQ=255)                                    aCGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAgg                                                                                                    >  2:1375198/1‑140 (MQ=255)                                    aCGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAgg                                                                                                    >  2:915118/1‑140 (MQ=255)                                      gTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGtc                                                                                                  <  1:1281903/140‑1 (MQ=255)                                       ttGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGtca                                                                                                 <  1:1375198/140‑1 (MQ=255)                                        tGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGtcat                                                                                                >  2:968104/1‑140 (MQ=255)                                         gcgcTTCGGTTTCAATACGCTCTAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCCCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGtcatc                                                                                               >  2:1688769/1‑140 (MQ=255)                                         gcgcTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGtcatc                                                                                               >  2:985232/1‑140 (MQ=255)                                         gcgcTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGtcatc                                                                                               >  2:143650/1‑140 (MQ=255)                                         gcgcTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGtcatc                                                                                               >  1:1638452/1‑140 (MQ=255)                                            cTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATg                                                                                            <  1:915118/140‑1 (MQ=255)                                            cTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATg                                                                                            <  2:1020226/140‑1 (MQ=255)                                                 gTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTc                                                                                       <  1:1443427/140‑1 (MQ=255)                                                  tttCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTcc                                                                                      <  1:158890/140‑1 (MQ=255)                                                       aTACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAgcg                                                                                  <  1:538624/139‑1 (MQ=255)                                                       aTACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAgcg                                                                                  >  2:538624/1‑139 (MQ=255)                                                         aCGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCgg                                                                               <  2:1638452/140‑1 (MQ=255)                                                            cTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGa                                                                            >  1:1579941/1‑140 (MQ=255)                                                            cTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGa                                                                            <  1:722243/140‑1 (MQ=255)                                                             tCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGAc                                                                           >  2:1636144/1‑140 (MQ=255)                                                                aGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCAt                                                                        <  1:409095/140‑1 (MQ=255)                                                                aGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCAt                                                                        <  2:358284/140‑1 (MQ=255)                                                                aGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCCCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCAt                                                                        <  1:1688769/140‑1 (MQ=255)                                                                      ttGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGtt                                                                  <  2:839226/140‑1 (MQ=255)                                                                       tGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTa                                                                 >  1:138269/1‑140 (MQ=255)                                                                       tGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTa                                                                 >  1:791604/1‑140 (MQ=255)                                                                       tGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGAGCGCAGATCGTGAGAGATATCAGAAAGCAAACGCTGCTGACAGGACAGCATGCGCTCCAGAGCGGTGACCATCTGGTTa                                                                 >  2:1510600/1‑140 (MQ=255)                                                                         cTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCAt                                                                        <  1:1363197/131‑1 (MQ=255)                                                                         cTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCAt                                                                        >  1:96424/1‑131 (MQ=255)                                                                         cTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCAt                                                                        >  2:1363197/1‑131 (MQ=255)                                                                         cTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCAt                                                                        <  2:96424/131‑1 (MQ=255)                                                                          tttCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTaaaa                                                              <  2:1037964/140‑1 (MQ=255)                                                                                  cTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCAcc                                                      >  1:1550083/1‑140 (MQ=255)                                                                                      gacgGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCg                                                  <  2:565183/140‑1 (MQ=255)                                                                                         ggCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCa                                               <  1:1028903/140‑1 (MQ=255)                                                                                             cAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGa                                           <  2:1118365/140‑1 (MQ=255)                                                                                               gTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAAt                                         <  2:804827/140‑1 (MQ=255)                                                                                                     ccGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCtgt                                   <  1:968104/140‑1 (MQ=255)                                                                                                           ccAGTTGCAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGcccc                             <  2:1640606/140‑1 (MQ=255)                                                                                                                  cAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGccc                              <  2:1567276/132‑1 (MQ=255)                                                                                                                  cAGACGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGccc                              >  1:1567276/1‑132 (MQ=255)                                                                                                                     aCGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGtt                   >  1:1330952/1‑140 (MQ=255)                                                                                                                     aCGCGTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGtt                   >  1:1168791/1‑140 (MQ=255)                                                                                                                       gcgTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTcc                 <  2:244961/140‑1 (MQ=255)                                                                                                                         gTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCgg               >  2:1215776/1‑140 (MQ=255)                                                                                                                         gTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCgg               >  1:403786/1‑140 (MQ=255)                                                                                                                         gTCAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCgg               >  1:1165766/1‑140 (MQ=255)                                                                                                                           cAGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGt             <  2:1025326/140‑1 (MQ=255)                                                                                                                            aGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCGCCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTg            <  1:405716/140‑1 (MQ=255)                                                                                                                            aGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTg            <  1:1577510/140‑1 (MQ=255)                                                                                                                            aGCGGGGTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTg            <  1:1636144/140‑1 (MQ=255)                                                                                                                                gggTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTgg        <  2:1165766/140‑1 (MQ=255)                                                                                                                                 ggTGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCtagc       <  2:1168791/140‑4 (MQ=255)                                                                                                                                   tGCGCAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGt     >  1:160355/1‑140 (MQ=255)                                                                                                                                    gcgcAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTa    >  1:649746/1‑140 (MQ=255)                                                                                                                                     cgcAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTaa   >  2:1699393/1‑140 (MQ=255)                                                                                                                                      gcAGATCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAg  <  1:1215776/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        GACATCACCAACAGATCGTTGATCATGCTGTCCAGACGTTGCGCTTCGGTTTCAATACGCTCCAGTTCCTTGCTTTCACCGCTACGACGGCGCAGTAACGCCGTACCCAGTTGCAGACGCGTCAGCGGGGTGCGCAGCTCGTGAGAGATATCAGAAAGCAGACGCTGCTGAGAGGTCATCATGCGCTCCAGCGCGGTGACCATCTGGTTAAAACTGGCACCTGCGGCAAGGAATTCCTGTGGCCCCGCTTCCAGTTCCGGGTGCTGGCGTAAG  >  NC_000913/4104092‑4104364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑