Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,786,057	C→A	L352L (CTG→CTT)	ppsA ←	phosphoenolpyruvate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,786,057	0	C	A	100.0%	290.3 / NA	82	L352L (CTG→CTT)	ppsA	phosphoenolpyruvate synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (34/48);  total (34/48)

TCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGCAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATT  >  NC_000913/1785924‑1786189                                                                                                                                      |                                                                                                                                     tcATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGta                                                                                                                                >  1:1306986/1‑140 (MQ=255)    tGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATaa                                                                                                                             <  2:525529/140‑1 (MQ=255)     gTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAAc                                                                                                                            <  2:1660302/140‑1 (MQ=255)          tAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTcc                                                                                                                       >  2:719255/1‑140 (MQ=255)          tAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTcc                                                                                                                       >  2:1248279/1‑140 (MQ=255)          tAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTcc                                                                                                                       >  2:1556406/1‑140 (MQ=255)               aGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCAtgac                                                                                                                  >  1:1719549/1‑140 (MQ=255)                 cACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCAtgacct                                                                                                                >  2:923877/1‑140 (MQ=255)                    gTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCAtgacctgac                                                                                                             <  1:15118/140‑1 (MQ=255)                      cGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAAc                                                                                                                            <  2:2964/123‑1 (MQ=255)                      cGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAAc                                                                                                                            >  1:2964/1‑123 (MQ=255)                        ccAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACcgcg                                                                                                         >  2:1273033/1‑140 (MQ=255)                        ccAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACcgcg                                                                                                         >  1:1107050/1‑140 (MQ=255)                         cAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGt                                                                                                        >  2:1661506/1‑140 (MQ=255)                         cAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGt                                                                                                        >  1:688605/1‑140 (MQ=255)                           ggTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGa                                                                                                      >  2:783588/1‑140 (MQ=255)                                gATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCa                                                                                                 <  1:694047/140‑1 (MQ=255)                                gATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCa                                                                                                 <  1:783588/140‑1 (MQ=255)                                gATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCa                                                                                                 <  1:923877/140‑1 (MQ=255)                                gATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCa                                                                                                 <  2:1037393/140‑1 (MQ=255)                                gATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCa                                                                                                 <  2:1107050/140‑1 (MQ=255)                                gATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCa                                                                                                 <  2:1719549/140‑1 (MQ=255)                                  tGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACg                                                                                               >  2:483356/1‑140 (MQ=255)                                      gTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGttt                                                                                           >  2:1219313/1‑140 (MQ=255)                                      gTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGttt                                                                                           <  2:270780/140‑1 (MQ=255)                                       ttCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTc                                                                                          >  1:1017670/1‑140 (MQ=255)                                           tttCGCTGATGTCATGGATGACTTTCTCCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGa                                                                                      <  2:1143398/140‑1 (MQ=255)                                           tttCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGa                                                                                      <  1:1499946/140‑1 (MQ=255)                                           tttCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGa                                                                                      <  1:1576194/140‑1 (MQ=255)                                           tttCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGa                                                                                      <  1:237562/140‑1 (MQ=255)                                           tttCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGa                                                                                      <  1:562026/140‑1 (MQ=255)                                            ttCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGAc                                                                                     >  1:1524834/1‑140 (MQ=255)                                                cTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACgcgc                                                                                 >  2:444752/1‑140 (MQ=255)                                                 tGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGcc                                                                                >  2:365369/1‑140 (MQ=255)                                                  gATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCt                                                                               <  2:1099224/140‑1 (MQ=255)                                                  gATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCt                                                                               <  2:1707504/140‑1 (MQ=255)                                                  gATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCt                                                                               <  1:1661506/140‑1 (MQ=255)                                                     gTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGca                                                                            <  1:390231/140‑1 (MQ=255)                                                     gTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGca                                                                            <  1:365369/140‑1 (MQ=255)                                                          ggATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATg                                                                       <  2:1306986/140‑1 (MQ=255)                                                            aTGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGaa                                                                     <  2:444567/140‑1 (MQ=255)                                                            aTGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGaa                                                                     <  2:274060/140‑1 (MQ=255)                                                            aTGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGaa                                                                     <  1:414717/140‑1 (MQ=255)                                                              gACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACa                                                                   >  1:971058/1‑140 (MQ=255)                                                                cTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGt                                                                 >  1:1583684/1‑140 (MQ=255)                                                                  ttCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATg                                                                       >  2:86777/1‑132 (MQ=255)                                                                  ttCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATg                                                                       <  1:86777/132‑1 (MQ=255)                                                                   tCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTa                                                              >  2:75306/1‑140 (MQ=255)                                                                    cACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTAc                                                             <  2:1607221/140‑1 (MQ=255)                                                                    cACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTAc                                                             <  1:742931/140‑1 (MQ=255)                                                                    cACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTAc                                                             <  2:537553/140‑1 (MQ=255)                                                                        ggACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGgt                                                         <  2:971058/140‑1 (MQ=255)                                                                         gACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGgtg                                                        <  2:810923/140‑1 (MQ=255)                                                                             cGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGc                                                    <  1:483356/140‑1 (MQ=255)                                                                              gCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGcc                                                   <  1:1219313/140‑1 (MQ=255)                                                                              gCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGcc                                                   <  1:75306/140‑1 (MQ=255)                                                                                aCCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCAt                                                 <  1:537855/140‑1 (MQ=255)                                                                                       cgatgACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGc                                          <  2:109146/140‑1 (MQ=255)                                                                                        gatgACCGATAGCACGGCCTTCGGCGATAATCTTCCCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGcc                                         <  1:1670703/140‑1 (MQ=255)                                                                                        gatgACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGcc                                         <  2:1583684/140‑1 (MQ=255)                                                                                        gatgACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGcc                                         <  1:1273033/140‑1 (MQ=255)                                                                                        gatgACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGcc                                         <  2:47279/140‑1 (MQ=255)                                                                                              cGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCa                                   >  1:591299/1‑140 (MQ=255)                                                                                                   gCACGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATc                              >  1:1099261/1‑140 (MQ=255)                                                                                                     aCGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCa                            <  2:1017670/140‑1 (MQ=255)                                                                                                      cGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCAt                           >  1:278577/1‑140 (MQ=255)                                                                                                      cGGCCTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCAt                           >  1:534622/1‑140 (MQ=255)                                                                                                         ccTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCgg                        >  2:1528194/1‑140 (MQ=255)                                                                                                         ccTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCgg                        >  1:884435/1‑140 (MQ=255)                                                                                                          cTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCggg                       <  1:1556406/140‑1 (MQ=255)                                                                                                          cTTCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCggg                       <  1:719255/140‑1 (MQ=255)                                                                                                           ttCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGc                      <  2:108672/140‑1 (MQ=255)                                                                                                            tCGGCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCg                     >  1:127023/1‑140 (MQ=255)                                                                                                              ggCGATAATCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGAc                   <  2:591299/140‑1 (MQ=255)                                                                                                                     aTCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGc                      >  2:1546049/1‑130 (MQ=255)                                                                                                                     aTCTTACCCTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGc                      <  1:1546049/130‑1 (MQ=255)                                                                                                                      tCTTTCCCTGTGAATGAAGCGTATAACGCTCCATGACCTGGCCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTGCCGGTGTGGAAATCTTTCGCCCACTCAATAACCATCGGGCGACCGTAgtgt           <  2:615977/140‑1 (MQ=255)                                                                                                                             cTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGAGCGTAGTGTTTCTCaa    <  2:1092780/140‑1 (MQ=255)                                                                                                                             cTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCaa    >  1:990846/1‑140 (MQ=255)                                                                                                                             cTGTGAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCaa    >  2:408547/1‑140 (MQ=255)                                                                                                                               gtgAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCg                 <  1:177117/125‑1 (MQ=255)                                                                                                                               gtgAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCg                 >  2:177117/1‑125 (MQ=255)                                                                                                                               gtgAATGAAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAAtt  >  1:1223321/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                     TCATGTCAGTAACCAGCACGTCGCCAGGTTCGATGCGGTTCATTTCGCTGATGTCATGGATGACTTTCACCGGACCCGCACCGATGCGATGACCGATAGCACGGCCTTCGGCGATAATCTTACCCTGTGAATGCAGCGTATAACGCTCCATGACCTGACCGCGTGAGCGCACGGTTTCCGGACGCGCCTGCACAATGAACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATT  >  NC_000913/1785924‑1786189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑