Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,863,372	C→A	S201Y (TCC→TAC)	gapA →	glyceraldehyde‑3‑phosphate dehydrogenase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,863,372	0	C	A	100.0%	125.1 / NA	37	S201Y (TCC→TAC)	gapA	glyceraldehyde‑3‑phosphate dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/19);  total (18/19)

GCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGAC  >  NC_000913/1863236‑1863496                                                                                                                                         |                                                                                                                             gCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCa                                                                                                                           >  2:376472/1‑140 (MQ=255)   tCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGa                                                                                                                         <  1:381415/140‑1 (MQ=255)       cTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAAcat                                                                                                                     >  1:797123/1‑140 (MQ=255)              aaGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCg                                                                                                              <  1:409078/140‑1 (MQ=255)              aaGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCg                                                                                                              <  2:276727/140‑1 (MQ=255)                    tCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCctct                                                                                                        >  1:113282/1‑140 (MQ=255)                    tCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCctct                                                                                                        >  1:476565/1‑140 (MQ=255)                      aaCGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGGACATCATCCCGTCCTCTAc                                                                                                      >  2:711781/1‑140 (MQ=255)                      aaCGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTAc                                                                                                      >  1:765117/1‑140 (MQ=255)                        cGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCg                                                                                                              >  1:455492/1‑130 (MQ=255)                        cGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCg                                                                                                              <  2:455492/130‑1 (MQ=255)                          aTAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGt                                                                                                  >  1:870472/1‑140 (MQ=255)                                 cGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTa                                                                                           <  2:870472/140‑1 (MQ=255)                                 cGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTa                                                                                           <  2:416323/140‑1 (MQ=255)                                  ggCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTaa                                                                                          >  1:16586/1‑140 (MQ=255)                                    catcatCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAg                                                                                        <  2:552647/140‑1 (MQ=255)                                      tcatcGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCt                                                                                      <  2:765117/140‑1 (MQ=255)                                      tcatcGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCt                                                                                      <  1:376472/140‑1 (MQ=255)                                        atcGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGt                                                                                    <  2:326070/140‑1 (MQ=255)                                            aaGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGt                                                                                <  1:463003/140‑1 (MQ=255)                                                 cTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGt                                                                           <  2:476565/140‑1 (MQ=255)                                                  tgatgaCCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCt                                                                                      <  2:90708/128‑1 (MQ=255)                                                  tgatgaCCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCt                                                                                      >  1:90708/1‑128 (MQ=255)                                                        ccaccGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCa                                                                    <  2:716654/140‑1 (MQ=255)                                                         caccGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAg                                                                   >  2:682441/1‑140 (MQ=255)                                                          accGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGa                                                                  >  1:290012/1‑140 (MQ=255)                                                            cGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCAGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAAc                                                                >  1:669492/1‑140 (MQ=255)                                                                            gCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactgac                                                <  2:624123/140‑1 (MQ=255)                                                                             cTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGGACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactgact                                               >  2:733522/1‑140 (MQ=255)                                                                             cTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAactgact                                               >  2:363273/1‑140 (MQ=255)                                                                               aCTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTgg                                             >  2:68322/1‑140 (MQ=255)                                                                                       aaCCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGgcgt                                     >  2:777463/1‑140 (MQ=255)                                                                                                tGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGgcgttccgcgttc                            <  1:682441/140‑1 (MQ=255)                                                                                                     cGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGAcc                       <  1:68322/140‑1 (MQ=255)                                                                                                          cacaAAGACTGGCGCGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGaa                  <  1:777463/140‑1 (MQ=255)                                                                                                                     gcgcGGCGGCCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAg       >  1:775750/1‑140 (MQ=255)                                                                                                                          gcggcCGCGGCGCTTACCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTtgac  <  2:797123/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                             GCTCCGCTGGCTAAAGTTATCAACGATAACTTCGGCATCATCGAAGGTCTGATGACCACCGTTCACGCTACTACCGCTACTCAGAAAACCGTTGATGGCCCGTCTCACAAAGACTGGCGCGGCGGCCGCGGCGCTTCCCAGAACATCATCCCGTCCTCTACCGGTGCTGCTAAAGCTGTAGGTAAAGTACTGCCAGAACTGAATGGCAAACTGACTGGTATGGCGTTCCGCGTTCCGACCCCGAACGTATCTGTAGTTGAC  >  NC_000913/1863236‑1863496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑