Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,899,261	G→T	A53E (GCG→GAG)	ygcU ←	putative FAD‑containing dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,899,261	0	G	T	100.0%	198.2 / NA	56	A53E (GCG→GAG)	ygcU	putative FAD‑containing dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/31);  total (25/31)

AGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTCCTTTAGCTGGTC  >  NC_000913/2899130‑2899391                                                                                                                                    |                                                                                                                                   aGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCGTAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGa                                                                                                                            >  2:278549/1‑140 (MQ=255) aGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGa                                                                                                                            >  1:539605/1‑140 (MQ=255) aGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGa                                                                                                                            >  1:772063/1‑140 (MQ=255) aGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGa                                                                                                                            <  2:68264/140‑1 (MQ=255)      tCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCgg                                                                                                                       <  2:135161/140‑1 (MQ=255)      tCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCgg                                                                                                                       >  1:511043/1‑140 (MQ=255)      tCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCgg                                                                                                                       <  1:873568/140‑1 (MQ=255)        tACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCa                                                                                                                     <  2:590489/140‑1 (MQ=255)         acaacaGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCaa                                                                                                                    <  1:575937/140‑1 (MQ=255)                tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATaa                                                                                                             >  1:576086/1‑140 (MQ=255)                tttCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATaa                                                                                                             >  2:23316/1‑140 (MQ=255)                  tCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAAt                                                                                                           >  2:181500/1‑140 (MQ=255)                    cAGCCCGCCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGc                                                                                                         <  2:655256/140‑1 (MQ=255)                       cccACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCAt                                                                                                      >  1:163774/1‑140 (MQ=255)                            cTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAata                                                                                                 >  2:785919/1‑140 (MQ=255)                                 gTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCgg                                                                                            <  2:106528/140‑1 (MQ=255)                                    gCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGaaa                                                                                         >  2:56428/1‑140 (MQ=255)                                     cGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAAt                                                                                        >  1:706305/1‑140 (MQ=255)                                      ggAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAAtt                                                                                       >  1:668940/1‑140 (MQ=255)                                      ggAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAAtt                                                                                       >  1:107341/1‑140 (MQ=255)                                       gAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAAttt                                                                                      >  2:682023/1‑140 (MQ=255)                                           cACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGa                                                                                  <  1:7207/140‑1 (MQ=255)                                                  aCGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGt                                                                           <  2:772063/140‑1 (MQ=255)                                                  aCGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGt                                                                           <  2:695896/140‑1 (MQ=255)                                                  aCGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGt                                                                           <  1:391867/140‑1 (MQ=255)                                                   cgcgGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTc                                                                          <  1:56428/140‑1 (MQ=255)                                                           acCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGt                                                                  >  2:418358/1‑140 (MQ=255)                                                           acCGTTAA‑TTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGtt                                                                 >  1:873454/1‑140 (MQ=255)                                                             cGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                                                <  1:418358/140‑1 (MQ=255)                                                             cGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                                                <  2:706305/140‑1 (MQ=255)                                                             cGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttt                                                                <  2:140137/140‑1 (MQ=255)                                                               ttAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGttttt                                                              <  2:88788/140‑1 (MQ=255)                                                                  aTTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCtt                                                           <  1:23316/140‑1 (MQ=255)                                                                  aTTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCtt                                                           <  2:511043/140‑1 (MQ=255)                                                                    tttGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTa                                                         >  1:434920/1‑140 (MQ=255)                                                                       gtgCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATa                                                      <  1:814436/140‑1 (MQ=255)                                                                       gtgCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATa                                                      >  1:384894/1‑140 (MQ=255)                                                                            cATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGtt                                                 <  1:634925/140‑1 (MQ=255)                                                                                cATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCAt                                             <  2:821084/140‑1 (MQ=255)                                                                                   aaaaTTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCgg                                          <  2:873454/140‑1 (MQ=255)                                                                                   aaaaTTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCgg                                          <  1:181500/140‑1 (MQ=255)                                                                                     aaTTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTa                                        <  2:226572/140‑1 (MQ=255)                                                                                        tCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAAtt                                     <  1:785919/140‑1 (MQ=255)                                                                                          aGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTAc                                   <  2:107341/140‑1 (MQ=255)                                                                                           gCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTAcg                                  >  2:430371/1‑140 (MQ=255)                                                                                           gCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTAcg                                  >  2:421521/1‑140 (MQ=255)                                                                                                    aTACTTGCTCTGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCa                         <  1:353299/140‑1 (MQ=255)                                                                                                           ctctGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACcaacaa                  <  1:683468/140‑1 (MQ=255)                                                                                                            tctGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAAt                 <  2:163774/140‑1 (MQ=255)                                                                                                             ctGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCAc                        >  2:135407/1‑132 (MQ=255)                                                                                                             ctGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCAc                        <  1:135407/132‑1 (MQ=255)                                                                                                             ctGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAAtt                >  2:479353/1‑140 (MQ=255)                                                                                                             ctGTGGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAAtt                >  2:196708/1‑140 (MQ=255)                                                                                                               gtgGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTc              >  2:162088/1‑140 (MQ=255)                                                                                                                tgGAACCGAGTTTTACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTcc             >  1:291544/1‑140 (MQ=255)                                                                                                                           tttACGACCTCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTCCTTTAGCTGGTc  <  1:278549/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                   AGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCATGAATATCCGGAAATTTACGAAAACGGTCAATACTGTTTTTCTTTAATACTGTTTCATCGGTAATTACGCGATCTGCACCAACAATTTCCTTTAGCTGGTC  >  NC_000913/2899130‑2899391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑