Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,619,483	G→T	E122* (GAA→TAA)	marR →	DNA‑binding transcriptional repressor MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,619,483	0	G	T	100.0%	184.7 / NA	54	E122* (GAA→TAA)	marR	DNA‑binding transcriptional repressor MarR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (23/31);  total (23/31)

CCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATT  >  NC_000913/1619350‑1619615                                                                                                                                      |                                                                                                                                     ccTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTaa                                                                                                                                >  1:831793/1‑140 (MQ=255)        tGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACaaaaaa                                                                                                                         <  1:154210/140‑1 (MQ=255)          tAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAAcc                                                                                                                       >  2:138197/1‑140 (MQ=255)               gCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGAcg                                                                                                                  >  2:179298/1‑140 (MQ=255)               gCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGAcg                                                                                                                  >  2:122343/1‑140 (MQ=255)               gCTGGGCGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAATTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGAcg                                                                                                                  >  2:386173/1‑140 (MQ=255)                cTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGAcgg                                                                                                                 <  1:728172/140‑1 (MQ=255)                    gTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGa                                                                                                             >  2:703908/1‑140 (MQ=255)                    gTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGa                                                                                                             >  2:642634/1‑140 (MQ=255)                    gTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGa                                                                                                             >  2:452972/1‑140 (MQ=255)                    gTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGa                                                                                                             >  1:410020/1‑140 (MQ=255)                       gAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGa                                                                                                          <  1:338074/140‑1 (MQ=255)                        aaaGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGaa                                                                                                         <  1:703908/140‑1 (MQ=255)                         aaGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAg                                                                                                        >  1:461456/1‑140 (MQ=255)                          aGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGt                                                                                                       <  1:138197/140‑1 (MQ=255)                          aGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGt                                                                                                       >  1:807740/1‑140 (MQ=255)                          aGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGt                                                                                                       <  1:146799/140‑1 (MQ=255)                            gTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTgg                                                                                                     <  1:608427/140‑1 (MQ=255)                               gCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCaa                                                                                                  <  1:248597/140‑1 (MQ=255)                               gCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGACAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGc                                                                                                    <  2:230309/138‑1 (MQ=255)                               gCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGACAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGc                                                                                                    >  1:230309/1‑138 (MQ=255)                               gCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGACGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCaa                                                                                                  <  1:125311/140‑1 (MQ=255)                                ccGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAac                                                                                                 <  2:535497/140‑1 (MQ=255)                                 cGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAaca                                                                                                >  1:645557/1‑140 (MQ=255)                                    aCCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGGGGACGAAGTGGCAACACtt                                                                                             >  2:827012/1‑140 (MQ=255)                                          aTGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCGCCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTAt                                                                                       <  2:645557/140‑1 (MQ=255)                                             aCAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTg                                                                                    <  1:653160/140‑1 (MQ=255)                                               aaGCGCGGCGTACTGGTAAAGCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCt                                                                                  <  1:386173/140‑1 (MQ=255)                                               aaGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCt                                                                                  <  2:463069/140‑1 (MQ=255)                                                                        ccaccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACaaaa                                                         >  2:851069/1‑140 (MQ=255)                                                                           ccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAACCCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAag                                                      <  2:461456/140‑1 (MQ=255)                                                                           ccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAag                                                      <  1:817976/140‑1 (MQ=255)                                                                           ccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAag                                                      <  1:452972/140‑1 (MQ=255)                                                                           ccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAag                                                      <  1:525872/140‑1 (MQ=255)                                                                           ccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAag                                                      <  1:642634/140‑1 (MQ=255)                                                                             ggcggcGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAagag                                                    <  2:410020/140‑1 (MQ=255)                                                                              gcggcgcggcAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAgg                                                   >  1:811815/1‑140 (MQ=255)                                                                              gcggcgcggcAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAgg                                                   >  2:892116/1‑140 (MQ=255)                                                                                  cgcggcAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATg                                               >  2:335839/1‑140 (MQ=255)                                                                                     ggcAATATGTGAACAATGCCATCAATTAGTTGGCCAGGCCCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACGCTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACg                                            <  1:53359/140‑1 (MQ=255)                                                                                     ggcAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACg                                            <  1:179298/140‑1 (MQ=255)                                                                                           atGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTcc                                      <  2:807740/140‑1 (MQ=255)                                                                                                aaCAATGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAACCCTGACGGCGGACGAAGGGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACg                                 <  2:91904/140‑1 (MQ=255)                                                                                                   actGCCTTCAATTGATTGACAAGGACCTGCACCAAAAACAACCAAAAAACTTGGCGGTGGAGGAAGTGGAAATTGTTGAGTATTAGCTTAAGAAATTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCaa                              <  2:214576/138‑1 (MQ=255)                                                                                                     tGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGc                                <  2:561555/136‑1 (MQ=255)                                                                                                     tGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGc                                >  1:561555/1‑136 (MQ=255)                                                                                                     tGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATa                            >  2:493779/1‑140 (MQ=255)                                                                                                     tGCCATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATa                            >  1:220923/1‑140 (MQ=255)                                                                                                     tGCCATCAATTAGTTGCCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGACCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATa                            >  1:214576/1‑140 (MQ=255)                                                                                                       ccATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACt                          <  2:811815/140‑1 (MQ=255)                                                                                                       ccATCAATTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACt                          <  1:493779/140‑1 (MQ=255)                                                                                                            aaTTAGTTGGCCAGGACCTGCACCAATAATTAACAAAAACCCTGACGGCGGACGAAGTGGCAACCCTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGAAGc                     <  2:402280/140‑1 (MQ=255)                                                                                                                 gTTGGCCAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTa                <  1:892116/140‑1 (MQ=255)                                                                                                                       cAGGACCTGCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTc          >  2:443971/1‑140 (MQ=255)                                                                                                                          gcccTGCACAAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATa       <  1:838851/138‑1 (MQ=255)                                                                                                                               gCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCAtt  <  2:112554/140‑1 (MQ=255)                                                                                                                               gCACCAATAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCAtt  >  1:749585/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                     CCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGGCAACACTTGAGTATTTGCTTAAGAAAGTCCTGCCGTAAACAAAAAAGAGGTATGACGATGTCCAGACGCAATACTGACGCTATTACCATTCATAGCATT  >  NC_000913/1619350‑1619615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑