Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913372,6400TG23.6% 210.5 / 24.1 89L176V (TTA→GTA) mhpD2‑hydroxypentadienoate hydratase
Reads supporting (aligned to +/- strand):  ref base T (20/48);  new base G (21/0);  total (41/48)
Fisher's exact test for biased strand distribution p-value = 2.07e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 103 total aligned reads displayed.

CATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAA  >  NC_000913/372501‑372774
                                                                                                                                            |                                                                                                                                      
cATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGt                                                                                                                                        <  2:1312223/140‑1 (MQ=255)
cATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGt                                                                                                                                        >  2:583265/1‑140 (MQ=255)
  ttGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCCACGTTCCGGGGGGGtt                                                                                                                                       >  2:1983788/1‑140 (MQ=255)
  ttGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTa                                                                                                                                      >  2:617653/1‑140 (MQ=255)
  ttGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTa                                                                                                                                      >  2:1812540/1‑140 (MQ=255)
  ttGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTa                                                                                                                                      >  1:1436946/1‑140 (MQ=255)
  ttGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCCGGGCAACG‑CCCCGCGGGGGTa                                                                                                                                      >  2:668115/1‑140 (MQ=255)
   tGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAg                                                                                                                                     <  1:617653/140‑1 (MQ=255)
   tGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAg                                                                                                                                     <  1:1544181/140‑1 (MQ=255)
       tGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGGGCAACG‑TCCGGCGGGGGTAGAccc                                                                                                                                 >  2:1339019/1‑139 (MQ=255)
       tGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCt                                                                                                                                 >  2:1267566/1‑140 (MQ=255)
          gTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGaa                                                                                                                              >  1:922900/1‑140 (MQ=255)
          gTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCCGCGCCACG‑TCCGGCGGGGGTAGACCTGaa                                                                                                                              >  2:1709259/1‑140 (MQ=255)
            aCTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGaaaa                                                                                                                            >  1:1971063/1‑140 (MQ=255)
              ttCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAAc                                                                                                                          >  2:2009905/1‑140 (MQ=255)
               tCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACt                                                                                                                         >  1:423872/1‑140 (MQ=255)
                ccGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTg                                                                                                                        <  2:1245555/140‑1 (MQ=255)
                 cGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTgc                                                                                                                       >  1:1960358/1‑140 (MQ=255)
                   gcgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTgcgc                                                                                                                     >  1:807677/1‑140 (MQ=255)
                   gcgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTgcgc                                                                                                                     <  2:884349/140‑1 (MQ=255)
                   gcgcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTgcgc                                                                                                                     <  1:1983788/140‑1 (MQ=255)
                     gcTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCa                                                                                                                   >  2:1729681/1‑140 (MQ=255)
                       tGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATg                                                                                                                 <  1:1166698/140‑1 (MQ=255)
                           aGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGa                                                                                                             <  2:807677/140‑1 (MQ=255)
                                tggGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGAcg                                                                                                        <  1:1339019/140‑1 (MQ=255)
                                 ggggAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGAcgc                                                                                                       >  1:169519/1‑140 (MQ=255)
                                  gggAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGAcgcg                                                                                                      >  1:528865/1‑140 (MQ=255)
                                   ggAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCGGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCCGGCCAAAG‑TCCGGCGGGGGTTGACCTGGAAAACTACGCCAAGAAGAGGACGCGt                                                                                                     >  2:1268787/1‑140 (MQ=255)
                                   ggAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGt                                                                                                     >  1:1686343/1‑140 (MQ=255)
                                   ggAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGt                                                                                                     >  1:312795/1‑140 (MQ=255)
                                    gAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGta                                                                                                    <  1:1812540/140‑1 (MQ=255)
                                     aGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGtaa                                                                                                   <  2:1976638/140‑1 (MQ=255)
                                          cATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACg                                                                                              <  2:1058622/140‑1 (MQ=255)
                                            ttCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGaa                                                                                            <  2:403916/140‑1 (MQ=255)
                                              cgcgACTGGTCGATTCAGTTTGTCGATGCCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAAGGAaga                                                                                          <  2:1455675/140‑1 (MQ=255)
                                                         gATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCCTGAAGATGACGCGGAATAAAGAAGAGGGTTCTAGCg                                                                               >  2:433401/1‑140 (MQ=255)
                                                           ttCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCggg                                                                             <  1:128381/140‑1 (MQ=255)
                                                           ttCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCggg                                                                             >  1:966830/1‑140 (MQ=255)
                                                                tttGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCgg                                                                        <  2:1686343/140‑1 (MQ=255)
                                                                tttGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCgg                                                                        >  2:1913854/1‑140 (MQ=255)
                                                                     cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAgtg                                                                   <  1:433401/140‑3 (MQ=255)
                                                                     cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCg                                                                   >  2:1800709/1‑140 (MQ=255)
                                                                     cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCg                                                                   <  1:2009905/140‑1 (MQ=255)
                                                                     cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCg                                                                   >  2:138070/1‑140 (MQ=255)
                                                                     cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCg                                                                   <  1:583265/140‑1 (MQ=255)
                                                                     cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCg                                                                   <  2:1894555/140‑1 (MQ=255)
                                                                     cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTTGACCTGAAAAAATGCGCCCTGAAGATGACGCGGAATAAAGAAAAGGGTTTTAGCGGGCGCGGCAGCg                                                                   >  2:508532/1‑140 (MQ=255)
                                                                     cGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGACCCGCGCAACG‑CCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGGGGGCGCGGCAGCg                                                                   >  2:1274563/1‑140 (MQ=255)
                                                                      gATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGa                                                                  <  1:1709259/140‑1 (MQ=255)
                                                                      gATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGa                                                                  <  2:1436946/140‑1 (MQ=255)
                                                                      gATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGa                                                                  <  1:880210/140‑1 (MQ=255)
                                                                        tACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAAt                                                                >  1:171533/1‑140 (MQ=255)
                                                                        tACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGGAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGaaa                                                                >  2:270222/1‑139 (MQ=255)
                                                                             tGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTg                                                           >  2:1135810/1‑140 (MQ=255)
                                                                             tGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTg                                                           >  1:1340398/1‑140 (MQ=255)
                                                                              ggCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGGAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTgg                                                          >  1:1295192/1‑140 (MQ=255)
                                                                                 aGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGAc                                                       <  1:76823/140‑1 (MQ=255)
                                                                                  gATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACa                                                      <  2:1977461/140‑1 (MQ=255)
                                                                                  gATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACa                                                      <  1:1928027/140‑1 (MQ=255)
                                                                                  gATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACa                                                      <  1:112632/140‑1 (MQ=255)
                                                                                  gATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACa                                                      <  1:459135/140‑1 (MQ=255)
                                                                                  gATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACa                                                      <  1:508532/140‑1 (MQ=255)
                                                                                   aTAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACAt                                                     <  2:312795/140‑1 (MQ=255)
                                                                                      aCGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGTGAATGCCTGGGACATCCg                                                  <  1:783772/140‑1 (MQ=255)
                                                                                       cGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGc                                                 <  2:169519/140‑1 (MQ=255)
                                                                                            cctGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTTTAGCGGGCGCGGCAGCGAATGCCTGGGGCATCCGCTTAAt                                            >  2:1071262/1‑140 (MQ=255)
                                                                                            cctGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAAAGCCTGGGGCATCCGCCTAAt                                            >  2:1600615/1‑140 (MQ=255)
                                                                                               gtgGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCg                                         <  1:1703103/140‑1 (MQ=255)
                                                                                               gtgGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCg                                         <  2:804345/140‑1 (MQ=255)
                                                                                               gtgGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCg                                         <  1:1558271/140‑1 (MQ=255)
                                                                                                tgGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCgg                                        <  1:1301139/140‑1 (MQ=255)
                                                                                                    gtgtATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGt                                    >  2:1862563/1‑140 (MQ=255)
                                                                                                     tgtATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTc                                   >  2:612377/1‑140 (MQ=255)
                                                                                                       tATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTTGACCTGAAAAACAGCGGCCTGAAGATGAAGCGGAAAAACGAAGAGGTTTTTAGCGGGCGCCGCAGCGAATGCCTGGGAGATCCGCTTAAAGCGGCCGTctg                                 >  2:1278370/1‑140 (MQ=255)
                                                                                                       tATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctg                                 >  2:239101/1‑140 (MQ=255)
                                                                                                          gTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctggct                              <  1:1673863/140‑1 (MQ=255)
                                                                                                          gTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGGAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGGCATCCGCTTAATGCGGCCGTctggct                              >  2:1243625/1‑140 (MQ=255)
                                                                                                           tCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctggctg                             <  2:1576276/140‑1 (MQ=255)
                                                                                                             aTCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTctggctggg                           >  1:196727/1‑139 (MQ=255)
                                                                                                             aTCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCCCGGCAGCGAAAGCCTGGGACATCCGCTTAAAGCGGCCGGCtgggtggc                           >  2:775829/1‑140 (MQ=255)
                                                                                                              tCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCa                          <  2:1898719/140‑1 (MQ=255)
                                                                                                              tCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCa                          >  1:1898719/1‑140 (MQ=255)
                                                                                                              tCGGCGGTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCa                          >  2:698501/1‑140 (MQ=255)
                                                                                                               cggcggTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCAc                         <  1:1135810/140‑1 (MQ=255)
                                                                                                               cggcggTCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGGAATAACGAAGAGGGTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCAc                         >  2:2031377/1‑140 (MQ=255)
                                                                                                                   ggTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCaa                     <  1:1267566/140‑1 (MQ=255)
                                                                                                                    gTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCaaa                    <  1:1268787/140‑1 (MQ=255)
                                                                                                                     tCCGGCGCAACG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCaaaa                   >  1:909199/1‑140 (MQ=255)
                                                                                                                     tCCGGCGCAACG‑TCCGGCGGGGGTAAACCTGAAAAACTGCGCCCTGAAGATTACGCGGAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGGATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCaaaa                   >  2:1688851/1‑140 (MQ=255)
                                                                                                                        ggCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATgg                <  2:171533/140‑1 (MQ=255)
                                                                                                                         gcgcAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGc               <  1:1800709/140‑1 (MQ=255)
                                                                                                                          cgcAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGcc              >  2:371584/1‑140 (MQ=255)
                                                                                                                            cAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAg            <  1:1354104/140‑1 (MQ=255)
                                                                                                                             aaCG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGt           <  1:239101/140‑1 (MQ=255)
                                                                                                                               cG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGt           >  1:139348/1‑138 (MQ=255)
                                                                                                                               cG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGt           <  2:139348/138‑1 (MQ=255)
                                                                                                                               cG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCt         >  1:169221/1‑140 (MQ=255)
                                                                                                                               cG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCt         >  1:947886/1‑140 (MQ=255)
                                                                                                                               cG‑TCCGGCGGGGGTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCt         >  2:146075/1‑140 (MQ=255)
                                                                                                                                       cggGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGaa  <  1:1862563/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                      
CATTGAATGGGTACTTCCGGCGCTGGAAGTGGTGGGGAGCCGCATTCGCGACTGGTCGATTCAGTTTGTCGATACCGTGGCAGATAACGCCTCCTGTGGGGTGTATGTCATCGGCGGTCCGGCGCAACG‑TCCGGCGGGGTTAGACCTGAAAAACTGCGCCATGAAGATGACGCGTAATAACGAAGAGGTTTCTAGCGGGCGCGGCAGCGAATGCCTGGGACATCCGCTTAATGCGGCCGTCTGGCTGGCACGCAAAATGGCCAGTCTGGGTGAA  >  NC_000913/372501‑372774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑