| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,825,061 | 0 | G | A | 27.0% | 71.8 / 11.0 | 37 | intergenic (‑124/+79) | ves/spy | HutD family protein Ves/ATP‑independent periplasmic chaperone |
| Reads supporting (aligned to +/- strand): ref base G (26/1); new base A (0/10); total (26/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.16e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGT‑AACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCT > NC_000913/1824930‑1825195 | tATTCCATCCCCGCCCCCGGCCTTTTTTTTTCTTTTTTTTCGTTCAGGT‑ACCGGTGGATGGTTATCGGCAACTGGGGTGTTTTTCGTT‑AGGGGTGATCAGTTTTTTTTTACAACTTTT‑TGAAAAGAAAAAAAAAAAccgc < 2:612818/140‑1 (MQ=255) tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGT‑AACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGa > 1:1419388/1‑140 (MQ=255) tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGT‑AACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGa > 1:2049422/1‑140 (MQ=255) tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGT‑AACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGCGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGa > 1:897303/1‑140 (MQ=255) tACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGT‑AACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCt > 1:2013120/1‑140 (MQ=255) tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGT‑AACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTc > 1:1994108/1‑140 (MQ=255) ccGCTCCCGGCCTTTTTTTTTCATTTTTATCGTTCAGGT‑ACCGGTGGTTGTTTTTCGGCAACTGGGGTGTTTTACGTT‑AGTGGTGATCAGGTTTTTTTTACACCTTTT‑GGAGAAGAAAAAAAAAAACCGCCGATCCTGTc < 2:352951/139‑1 (MQ=255) gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGT‑AACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTccc > 1:803503/1‑139 (MQ=255) gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGT‑AACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCa > 1:1230700/1‑140 (MQ=255) gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGT‑AACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCa > 2:1726582/1‑140 (MQ=255) cTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGT‑AACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACGGCCGATCCTGTCCAc > 1:2024061/1‑140 (MQ=255) ggCCCTTTTTTTTCTTTTTTATCGTTCAGGT‑ACCGGTGGATGTTTATCGGCAACTGGGGTTTTTTACGTT‑AGGGGTGATCTGGTTTTTTTTAAAAGTTTT‑TGAGAAGAAAAAAGAAACCCGCCGATCCTGTCCACCGCAt < 2:456165/140‑1 (MQ=255) ggCCCTTTTTTTTCATTTTTATCGTTAAGGT‑ACCGGTGGATGTTTATCGGCAACTGGGGTGATTTACGTT‑AGTGGTGATCGGGTTTTTTTTACATTTTTT‑TGAGAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCAt < 2:1419388/140‑1 (MQ=255) ggCCCGTTTTTTTCTTTTTTTTCTTTAAGGT‑ACCCGTGGATGTTTATCGGCACCTGGGGTGATTTACGTT‑AGTGGTGATCAGGTTTTTTTTACAATTTTT‑TGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAt < 2:544280/140‑1 (MQ=255) tttCTTTTCATTGTTA‑GGTTGGGGTGAGCCGTGGATGTTTAACGTC‑ACCCGGGTTTTTTTAGTTGATTGGTCCGCAGTTTTTTTTTA‑AACCGTTGAGAAAAAAGAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTg < 2:1994108/139‑1 (MQ=255) ttCTTTTCATTGTTATCGTTCAGGT‑AACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc > 1:810058/1‑140 (MQ=255) tttCATTTTTATCGTTCAGGT‑ACCGGTGGTTGTTTATGGGCACCTGGGGTGATTTACGTT‑AGTGGTGATCGGGTTTTTTTTCCACTTGTT‑GGAAAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgg < 2:1044234/140‑1 (MQ=255) ttCATTGTTATCGTTCAGGT‑AACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgga > 2:1347720/1‑139 (MQ=255) ggT‑ACCGGTGGATGTTTAGCGGCAACTGGGGTTTTTTATGTT‑GGTGGTGATCGGTTTTTTTTAACAATTTTT‑GGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg < 2:2049422/140‑1 (MQ=255) ggT‑AACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg > 1:1493032/1‑140 (MQ=255) aaCCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTc < 2:1223504/140‑1 (MQ=255) tCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCt > 1:1997212/1‑140 (MQ=255) cTCGGGTGATTTACGTT‑AGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat > 1:339381/1‑140 (MQ=255) cTCGGGTGATTTACGTT‑AGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat > 1:167029/1‑140 (MQ=255) gATTTCCGTT‑AGTGGTGATCAGTTTTTTTTTACAATTGTT‑GGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcag < 2:339381/140‑1 (MQ=255) gATTTACGTT‑GGGGGTGATCAGGTTTTTTTAACAATTGTT‑TGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcag < 2:753481/140‑1 (MQ=255) tggtgATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCa > 2:1418668/1‑140 (MQ=255) gATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt > 1:1504411/1‑140 (MQ=255) gATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt > 1:1410467/1‑140 (MQ=255) gATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt > 1:1913722/1‑140 (MQ=255) aTCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTa > 2:1845651/1‑140 (MQ=255) ggCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtt > 1:783877/1‑140 (MQ=255) gCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt > 1:484416/1‑140 (MQ=255) tttttttACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCCATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTg > 1:581429/1‑140 (MQ=255) tGTT‑TGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt < 1:1845651/140‑1 (MQ=255) tGTA‑TGAGAAAAGAAAAAAAAGCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt < 2:810058/140‑1 (MQ=255) gTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTc > 1:346313/1‑140 (MQ=255) gTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTc > 2:525121/1‑140 (MQ=255) tt‑tGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt > 2:1830455/1‑140 (MQ=255) tGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt > 2:1804438/1‑140 (MQ=255) aaaaaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg < 2:1997212/137‑1 (MQ=255) aaaaaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg < 1:1318382/137‑1 (MQ=255) gaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt > 1:221756/1‑140 (MQ=255) gaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt > 1:1056680/1‑140 (MQ=255) gaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt > 1:1781480/1‑140 (MQ=255) | TATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGT‑AACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTT‑AGTGGTGATCAGGCTTTTTTTACAACTGTT‑TGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCT > NC_000913/1824930‑1825195 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |