Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,040,668	G→T	intergenic (+32/+238)	rrfA → / ← mobB	5S ribosomal RNA/molybdopterin‑guanine dinucleotide biosynthesis adaptor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,040,668	0	G	T	100.0%	189.1 / NA	73	intergenic (+32/+238)	rrfA/mobB	5S ribosomal RNA/molybdopterin‑guanine dinucleotide biosynthesis adaptor protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (40/33);  total (40/33)

CGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGC  >  NC_000913/4040533‑4040797                                                                                                                                        |                                                                                                                                  cgcgGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGcc                                                                                                                               >  1:1494308/1‑140 (MQ=21) cgcgGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGcc                                                                                                                               >  2:26139/1‑140 (MQ=21) cgcgGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGcc                                                                                                                               >  2:438161/1‑140 (MQ=21)  gcgGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCt                                                                                                                              <  2:1554236/140‑1 (MQ=25)  gcgGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCt                                                                                                                              <  1:1961842/140‑1 (MQ=21)         tCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGttt                                                                                                                       <  1:78442/140‑1 (MQ=25)         tCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGttt                                                                                                                       >  2:198134/1‑140 (MQ=21)           ccACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTa                                                                                                                     >  1:857861/1‑140 (MQ=21)                  aCCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCtgttg                                                                                                              >  1:469572/1‑140 (MQ=21)                  aCCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCtg                                                                                                                 >  1:517844/1‑137 (MQ=21)                  aCCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCtg                                                                                                                 <  2:517844/137‑1 (MQ=21)                   ccccATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCtgttgt                                                                                                             <  1:697655/140‑1 (MQ=25)                      cATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGttgtttg                                                                                                          <  1:1880202/140‑1 (MQ=25)                       aTGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGttgtttgt                                                                                                         >  1:1667524/1‑140 (MQ=21)                        tGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTTGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTc                                                                                                        >  1:771883/1‑140 (MQ=21)                        tGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTc                                                                                                        >  1:1901277/1‑140 (MQ=21)                         gCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCg                                                                                                       >  1:757582/1‑140 (MQ=21)                             aaCTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGa                                                                                                   >  1:854479/1‑140 (MQ=21)                                tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACg                                                                                                <  1:198134/140‑1 (MQ=21)                                tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACg                                                                                                <  2:20502/140‑1 (MQ=21)                                tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACg                                                                                                <  1:615651/140‑1 (MQ=21)                                tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACg                                                                                                <  2:1018699/140‑1 (MQ=21)                                tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACg                                                                                                <  1:474802/140‑1 (MQ=21)                                tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACg                                                                                                <  2:635356/140‑1 (MQ=21)                                tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACg                                                                                                <  1:281442/140‑1 (MQ=21)                                tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACg                                                                                                <  1:193526/140‑1 (MQ=21)                                  aGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGct                                                                                              <  1:191767/140‑1 (MQ=21)                                     aGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCtctc                                                                                           <  1:438161/140‑1 (MQ=21)                                     aGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCtctc                                                                                           <  2:1000739/140‑1 (MQ=21)                                     aGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCtctc                                                                                           <  2:857861/140‑1 (MQ=21)                                     aGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCtctc                                                                                           <  2:530530/140‑1 (MQ=21)                                     aGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTGTCGTTTTATCTGTTGTTTGTCGGTGAACGCgctc                                                                                           <  2:1494308/140‑1 (MQ=255)                                       tGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCt                                                                                         <  2:215104/140‑1 (MQ=21)                                          aaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAg                                                                                      <  2:1901277/140‑1 (MQ=21)                                              ccGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTg                                                                                        <  1:482457/134‑1 (MQ=21)                                              ccGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTg                                                                                        >  2:482457/1‑134 (MQ=21)                                                    cgcCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAAt                                                                            <  1:1422984/140‑1 (MQ=21)                                                              aGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAg                                                                  <  2:1667524/140‑1 (MQ=21)                                                                    gggTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGAtt                                                            >  1:792645/1‑140 (MQ=25)                                                                     ggTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCgg                                                               >  2:1440205/1‑136 (MQ=25)                                                                     ggTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCgg                                                               <  1:1440205/136‑1 (MQ=21)                                                                     ggTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGAttt                                                           >  1:1123945/1‑140 (MQ=25)                                                                       tctcCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGa                                                         >  2:5164/1‑140 (MQ=25)                                                                       tctcCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGa                                                         >  1:980985/1‑140 (MQ=25)                                                                        ctcctcATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGaa                                                        <  2:1085469/140‑1 (MQ=21)                                                                         tcctcATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAAc                                                       >  1:248721/1‑140 (MQ=25)                                                                          cctcATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGAt                                                             >  1:638891/1‑133 (MQ=21)                                                                          cctcATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGAt                                                             <  2:638891/133‑1 (MQ=21)                                                                           ctcATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGt                                                     >  1:809055/1‑140 (MQ=18)                                                                                 cGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGaa                                               >  1:950048/1‑140 (MQ=17)                                                                                   agagTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGc                                             >  1:770886/1‑140 (MQ=17)                                                                                   agagTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGc                                             >  2:1486170/1‑140 (MQ=18)                                                                                   agagTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGc                                             >  2:1706604/1‑140 (MQ=18)                                                                                     aggaGGGAACTGCTAGGCAGCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCTTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGTGTAAGACAAATCCTCCGGGATCGGATTTGATCGTTGCGAAGCaa                                           <  2:950048/137‑1 (MQ=11)                                                                                     agTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGAGAGCGGATTTGAACGTTGCGAAGCaa                                           >  2:1903256/1‑140 (MQ=18)                                                                                      gTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAAc                                          <  1:26139/140‑1 (MQ=17)                                                                                        aGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACgg                                        >  1:603513/1‑140 (MQ=17)                                                                                         gggAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGc                                       <  2:228187/140‑1 (MQ=17)                                                                                         gggAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGc                                       >  1:171175/1‑140 (MQ=18)                                                                                         gggAACTGCAAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTGTTATCTGTTGTTTGTCGGTGATCGCTCTCCTGAGTAGGACAAATCCGACGGGAGCGGATTTGAACGTTGCGAAGCAGCGGc                                       <  2:736338/140‑1 (MQ=11)                                                                                          ggAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGcc                                      >  2:1628377/1‑140 (MQ=18)                                                                                              cTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGa                                  <  1:1486170/140‑1 (MQ=17)                                                                                              cTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGa                                  >  1:1994639/1‑140 (MQ=18)                                                                                                gCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAgg                                <  1:1415910/140‑1 (MQ=17)                                                                                                 ccAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAggg                               >  1:759710/1‑140 (MQ=18)                                                                                                 ccAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAggg                               >  1:494370/1‑140 (MQ=17)                                                                                                      cATCAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCg                          <  2:1537531/140‑1 (MQ=17)                                                                                                         cAAATAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGc                       >  1:317495/1‑140 (MQ=18)                                                                                                          aaaTAAAACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGcc                      >  2:454910/1‑139 (MQ=17)                                                                                                               aaaCGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCTGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGAGGCGGGCacgacg                 >  1:1915750/1‑140 (MQ=255)                                                                                                                aaCGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGAcgc                >  2:211123/1‑140 (MQ=18)                                                                                                                aaCGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGAcgc                >  1:663489/1‑140 (MQ=18)                                                                                                                aaCGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGAcgc                >  1:66771/1‑140 (MQ=18)                                                                                                                     aaGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGAcgcccgcc           >  1:228735/1‑140 (MQ=18)                                                                                                                              tCGGAAGACTTGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGc  >  1:335866/1‑140 (MQ=18)                                                                                                                                        |                                                                                                                                  CGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGC  >  NC_000913/4040533‑4040797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑