Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,111,7470GT31.7% 90.5 / 25.6 41N156K (AAC→AAA) yiiQDUF1454 domain‑containing protein YiiQ
Reads supporting (aligned to +/- strand):  ref base G (28/0);  new base T (0/13);  total (28/13)
Fisher's exact test for biased strand distribution p-value = 5.68e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATG  >  NC_000913/4111612‑4111885
                                                                                                                                        |                                                                                                                                           
ttGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGtttt                                                                                                                                         >  2:1152940/1‑140 (MQ=255)
               aCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTtagta                                                                                                                         >  2:856538/1‑140 (MQ=255)
               aCCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTtagta                                                                                                                         >  1:657965/1‑140 (MQ=255)
                   tCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGctg                                                                                                                     >  1:90964/1‑138 (MQ=255)
                   tCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGa                                                                                                                     >  1:901080/1‑140 (MQ=255)
                   tCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGa                                                                                                                     >  2:1028077/1‑140 (MQ=255)
                                  aGCCCCAGTTTAATCGGTACAACAGCGAAGGCCCCCCCTTTTC‑CGCGTTTGCCCGCAAAAACATAACGCAGTCCCCCTTTTGTCTGGGTGAAAT‑ACGTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTg                                                                                                      <  2:657965/140‑1 (MQ=255)
                                   gcgcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTgg                                                                                                     >  1:575479/1‑140 (MQ=255)
                                    cgcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGc                                                                                                    >  2:206932/1‑140 (MQ=255)
                                     gcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCt                                                                                                   >  1:895125/1‑140 (MQ=255)
                                     gcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCt                                                                                                   >  1:165306/1‑140 (MQ=255)
                                     cccAGTTTAATCGGTTAAACAGCGAGGGCCACCCCTTTTT‑CGCCGTTGTCCGCAAAAACATAACGCAGTGCCCCTTCTGTCTGGGTGTAATATTTTTTTTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCt                                                                                                   <  2:1006527/139‑1 (MQ=255)
                                      ccAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCtt                                                                                                  >  2:813915/1‑140 (MQ=255)
                                         gCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtgt                                                                                               >  2:772473/1‑140 (MQ=255)
                                          cTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtgtg                                                                                              >  1:616698/1‑140 (MQ=255)
                                              aTCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGtttt                                                                                          >  1:1227805/1‑140 (MQ=255)
                                              aTCGCTCCAACAGCGAGGGCCGCCCCTTTTT‑CGCTGTTGCCCACAAAAATATAACGCTTTGCCCTTTCTTTCTCGGTGTAATAGCGTTTTTTTTC‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGtttt                                                                                          <  2:127367/140‑1 (MQ=255)
                                                       aCAGGGAAGGCCACCCCCTTTC‑CGCCGTTGTCCGCGAAAACATAACGCAGTCCCCCTTCTGTCTCGGTGTAATACTGTTTTTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTaa                                                                                 <  2:536467/140‑1 (MQ=255)
                                                             gaGGCCACCCCTTTTG‑CGCCGTTTCCCGCAACAACATAACGCGGCGCCCCTTCTGCCTGGGTGTAATACTTTTTTTTTTC‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                           <  2:794532/139‑1 (MQ=255)
                                                             agggTCAGCCCTTTTC‑CGCGGTTGTCCGCGACAACATAACGCAGTGCCCTTTCTTTCTCGTTATAATATCTTTTTTTTTC‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                           <  2:949767/138‑1 (MQ=255)
                                                             aaGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATggggg                                                                           >  1:927806/1‑139 (MQ=255)
                                                             aaGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                           >  1:1111574/1‑140 (MQ=255)
                                                                ggccgCCCCTTTTCCGCGGTTGCCCGCGAAAAAAAAACGCGGTCCCCCTTCTGTCTGGGTGTAATAGCTTTTTTTTTC‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGa                                                                         <  2:255372/136‑1 (MQ=255)
                                                                   aGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAgtgt                                                                     >  2:1025278/1‑140 (MQ=255)
                                                                         tttc‑CGCCTTTGTCCGCGAAAACATACCCAAGTGCCCCTTCTGCCTGGGTGTAAAATCTTTTTTTTTC‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGAt                                                               <  2:901080/140‑1 (MQ=255)
                                                                          ttt‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGTTc                                                              >  1:47285/1‑140 (MQ=255)
                                                                          ttt‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATc                                                              >  1:42661/1‑140 (MQ=255)
                                                                          ttt‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATc                                                              >  2:1160618/1‑140 (MQ=255)
                                                                             ccGCCGTTGTCCGCGACAACATAACGCAGTGCCCCTTCTGTCTCGGTGTAATAACTTTTTTTTTC‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCAc                                                            <  1:1028077/139‑1 (MQ=255)
                                                                                  gTTGTCCGCGACAACATAACCCAGTGCACTTTCTGTCTGGGTGTAATAACTTTTTTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg                                                       <  1:1306050/140‑1 (MQ=255)
                                                                                    tgcccGCAAAAACATAGCGCGGTCCCCTTTCTGTCTGGGTGAAATATTTTTTTTTTTC‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGcc                                                     <  2:661594/137‑1 (MQ=255)
                                                                                    tGTCCGCAAAAACAAACCGCAGTCCCCCTTCTGTCTGGGTTTAATAATTTTTTTTTTC‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGcc                                                     <  2:119928/140‑1 (MQ=255)
                                                                                              aacaTAACGCAGTGCCCCTTCTGTCTCGGTGTAATACCTTTTTTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCt                                           <  1:206932/140‑1 (MQ=255)
                                                                                                        aGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgc                                 >  1:126123/1‑140 (MQ=255)
                                                                                                          tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  2:864523/1‑140 (MQ=255)
                                                                                                          tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  1:662737/1‑140 (MQ=255)
                                                                                                          tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  1:782153/1‑140 (MQ=255)
                                                                                                          tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  1:804365/1‑140 (MQ=255)
                                                                                                           gCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgcttt                              >  1:563257/1‑140 (MQ=255)
                                                                                                                    gTCTGGGTTTAATAACTTTTTTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttt                     <  1:856538/140‑1 (MQ=255)
                                                                                                                    gTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttt                     >  2:854058/1‑140 (MQ=255)
                                                                                                                        cGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGc                 >  2:164647/1‑140 (MQ=255)
                                                                                                                         ggTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGct                >  1:355581/1‑140 (MQ=255)
                                                                                                                           tgtAATACTTTTTTTTTTC‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctct              <  2:364296/140‑1 (MQ=255)
                                                                                                                           tgtAATAACTTTTTTTTTC‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctct              <  1:889932/140‑1 (MQ=255)
                                                                                                                              aatatttttttttttC‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGc           <  2:126123/133‑1 (MQ=255)
                                                                                                                                    gtttgtttTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTgg     >  1:1038789/1‑140 (MQ=255)
                                                                                                                                     tttgtttTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctc               <  2:320520/129‑1 (MQ=255)
                                                                                                                                     tttgtttTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctc               >  1:320520/1‑129 (MQ=255)
                                                                                                                                     tttgtttTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa    >  2:750561/1‑140 (MQ=255)
                                                                                                                                     tttgtttTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa    >  1:425491/1‑140 (MQ=255)
                                                                                                                                      ttgtttTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGAt   <  2:662737/140‑1 (MQ=255)
                                                                                                                                      ttgtttTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGAt   <  1:1160618/140‑1 (MQ=255)
                                                                                                                                       tgtttTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATg  <  2:42661/140‑1 (MQ=255)
                                                                                                                                       tgtttTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATg  >  2:1246020/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                           
TTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATG  >  NC_000913/4111612‑4111885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑