Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,484,032	C→A	A277A (GCC→GCA)	yheT →	putative hydrolase YheT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,484,032	0	C	A	100.0%	202.4 / NA	57	A277A (GCC→GCA)	yheT	putative hydrolase YheT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/29);  total (28/29)

TACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAAT  >  NC_000913/3483896‑3484163                                                                                                                                         |                                                                                                                                    tACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCaaaa                                                                                                                                  >  1:1014114/1‑140 (MQ=255) tACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCaaaa                                                                                                                                  >  1:1461/1‑140 (MQ=255)     tCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACg                                                                                                                              <  2:1278779/140‑1 (MQ=255)       gCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGAt                                                                                                                            <  1:998234/140‑1 (MQ=255)       gCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGAt                                                                                                                            <  1:297186/140‑1 (MQ=255)       gCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGAt                                                                                                                            <  2:381664/140‑1 (MQ=255)       gCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGAt                                                                                                                            <  1:1205563/140‑1 (MQ=255)       gCATCCGGGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGAt                                                                                                                            <  2:351351/140‑1 (MQ=255)        cATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATc                                                                                                                           <  1:577743/140‑1 (MQ=255)               gAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTAt                                                                                                                    <  2:1050383/140‑1 (MQ=255)                         aTCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAg                                                                                                          <  2:713409/140‑1 (MQ=255)                          tctcATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAgg                                                                                                         >  2:20663/1‑140 (MQ=255)                          tctcATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAgg                                                                                                         >  1:340702/1‑140 (MQ=255)                                    gCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGaa                                                                                               >  1:38064/1‑140 (MQ=255)                                                     cGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTccccc                                                                              <  2:38064/140‑1 (MQ=255)                                                        cGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGc                                                                           <  1:1142581/140‑1 (MQ=255)                                                          aCGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAg                                                                         >  1:1220467/1‑140 (MQ=255)                                                          aCGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAg                                                                         >  2:231931/1‑140 (MQ=255)                                                           cGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAgg                                                                        >  1:851142/1‑140 (MQ=255)                                                            gCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGt                                                                       <  1:2973/140‑1 (MQ=255)                                                            gCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGt                                                                       >  1:1212575/1‑140 (MQ=255)                                                                tCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGt                                                                       <  2:685514/136‑1 (MQ=255)                                                                tCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGt                                                                       >  1:685514/1‑136 (MQ=255)                                                                tCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAg                                                                   <  2:1212575/140‑1 (MQ=255)                                                                   aCTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCTCCCCGCAGGTGGAGTAt                                                                <  1:695311/140‑1 (MQ=255)                                                                      attatCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCaa                                                             >  1:804360/1‑140 (MQ=255)                                                                       ttatCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAac                                                            >  1:526043/1‑140 (MQ=255)                                                                               aGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGaa                                                    <  2:340702/140‑1 (MQ=255)                                                                               aGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGaa                                                    >  1:275669/1‑140 (MQ=255)                                                                                 tgtAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACa                                                  >  1:630868/1‑140 (MQ=255)                                                                                 tgtAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACa                                                  <  1:20663/140‑1 (MQ=255)                                                                                 tgtAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGAATCAACTGACTGAACa                                                  >  1:894807/1‑140 (MQ=255)                                                                                   tAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATg                                                >  2:1226871/1‑140 (MQ=255)                                                                                    aGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATgg                                               <  2:894807/140‑1 (MQ=255)                                                                                      cgcCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCg                                             >  2:1255649/1‑140 (MQ=255)                                                                                      cgcCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCg                                             >  1:1082592/1‑140 (MQ=255)                                                                                       gcCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCgg                                            <  1:519761/140‑1 (MQ=255)                                                                                       gcCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCgg                                            >  1:208686/1‑140 (MQ=255)                                                                                           tGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCAt                                        >  1:97714/1‑140 (MQ=255)                                                                                           tGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCAt                                        >  1:1125539/1‑140 (MQ=255)                                                                                           tGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCAt                                        >  1:782050/1‑140 (MQ=255)                                                                                           tGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCAt                                        >  1:507270/1‑140 (MQ=255)                                                                                           tGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCAt                                        >  2:769859/1‑140 (MQ=255)                                                                                            gCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCTCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATg                                       <  2:681763/140‑1 (MQ=255)                                                                                             ccGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGt                                      <  2:1220467/140‑1 (MQ=255)                                                                                                         cGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTgg                          <  2:782050/140‑1 (MQ=255)                                                                                                         cGGATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTgg                          <  1:741417/140‑1 (MQ=255)                                                                                                          ggATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGc                         <  1:395427/140‑1 (MQ=255)                                                                                                           gATCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCg                        >  2:436589/1‑140 (MQ=255)                                                                                                            aTCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCgg                       >  1:854107/1‑140 (MQ=255)                                                                                                            aTCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCgg                       <  1:127953/140‑1 (MQ=255)                                                                                                             tCGCCAAACCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCggg                      >  1:860034/1‑139 (MQ=255)                                                                                                                    aCCGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTAc               >  1:496694/1‑140 (MQ=255)                                                                                                                     ccGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCgg                       >  1:1166327/1‑131 (MQ=255)                                                                                                                     ccGACGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCgg                       <  2:1166327/131‑1 (MQ=255)                                                                                                                         cGCTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCAt          <  2:1082592/140‑1 (MQ=255)                                                                                                                           cTGATTATTCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATcc        <  2:208686/140‑1 (MQ=255)                                                                                                                              attattCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTGTTTGCGGTACATTACTTCATCCGCa     >  1:1230292/1‑140 (MQ=255)                                                                                                                                 attCACGCAAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAAt  <  1:1226871/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACATGGCGGTCATGTTGGCTTTATTGGCGGTACATTACTTCATCCGCAAAT  >  NC_000913/3483896‑3484163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑