Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,518,935	C→A	G44* (GGA→TGA)	aroK ←	shikimate kinase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,518,935	0	C	A	100.0%	290.8 / NA	82	G44* (GGA→TGA)	aroK	shikimate kinase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (44/38);  total (44/38)

TTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCCGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAACCAGAAAGATATTGCGTTTCTCTGCCATTTTTTCG  >  NC_000913/3518798‑3519071                                                                                                                                          |                                                                                                                                         ttCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAgg                                                                                                                                        >  1:400152/1‑140 (MQ=255) ttCACAGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAgg                                                                                                                                        >  2:70609/1‑140 (MQ=255)   cACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGtt                                                                                                                                      <  2:25363/140‑1 (MQ=255)      gggATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGt                                                                                                                                   <  2:296437/140‑1 (MQ=255)      gggATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGt                                                                                                                                   >  2:1231953/1‑140 (MQ=255)       ggATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGtt                                                                                                                                  <  1:366899/140‑1 (MQ=255)           ttCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTtctc                                                                                                                              >  1:1162598/1‑140 (MQ=255)             cacaGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCaa                                                                                                                            >  1:686976/1‑140 (MQ=255)              acaGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAAt                                                                                                                           <  1:634803/140‑1 (MQ=255)                 gagCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAAtctc                                                                                                                        <  2:435474/140‑1 (MQ=255)                     cgccgccAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCGTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGa                                                                                                                    >  2:1044018/1‑140 (MQ=255)                           cAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGaa                                                                                                              <  2:1162598/140‑1 (MQ=255)                                gCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTa                                                                                                         <  1:932489/140‑1 (MQ=255)                                  cAGCACAATACCCTGGTTCTCGGCCGACTCCTTGATGAACTTTTCGTCGCGATCGCGTAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGATCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTaaa                                                                                                       >  2:949673/1‑140 (MQ=255)                                     cacaATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAAtt                                                                                                    <  1:434113/140‑1 (MQ=255)                                     cacaATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAAtt                                                                                                    <  1:130429/140‑1 (MQ=255)                                     cacaATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAAtt                                                                                                    >  1:761348/1‑140 (MQ=255)                                     cacaATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAAtt                                                                                                    <  1:377876/140‑1 (MQ=255)                                        aaTACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCa                                                                                                 <  1:544372/140‑1 (MQ=255)                                             ccTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTg                                                                                            <  2:960920/140‑1 (MQ=255)                                             ccTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTg                                                                                            <  2:159552/140‑1 (MQ=255)                                               tGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAg                                                                                          >  1:951309/1‑140 (MQ=255)                                               tGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAg                                                                                          <  2:686976/140‑1 (MQ=255)                                               tGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAg                                                                                          >  1:677225/1‑140 (MQ=255)                                               tGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAg                                                                                          >  2:852203/1‑140 (MQ=255)                                                 tttCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAgtt                                                                                        >  1:451621/1‑140 (MQ=255)                                                 tttCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAgtt                                                                                        >  2:425429/1‑140 (MQ=255)                                                  ttCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAgttg                                                                                       <  2:1104099/140‑1 (MQ=255)                                                  ttCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAgttg                                                                                       >  2:4012/1‑140 (MQ=255)                                                  ttCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAgttg                                                                                       <  1:379380/140‑1 (MQ=255)                                                  ttCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAgttg                                                                                       <  1:425429/140‑1 (MQ=255)                                                   tctcGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAgttgt                                                                                      <  1:864326/140‑1 (MQ=255)                                                    ctcGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAgttgtt                                                                                     <  2:208122/140‑1 (MQ=255)                                                    ctcGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAgttgtt                                                                                     >  2:15901/1‑140 (MQ=255)                                                    ctcGGTCAACTCATTGATGACCTTTTCTTCGAGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAgttgtt                                                                                     >  2:1111979/1‑140 (MQ=255)                                                      cGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGa                                                                                   >  2:1124292/1‑140 (MQ=255)                                                       ggTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAg                                                                                  >  2:150078/1‑140 (MQ=255)                                                       ggTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAg                                                                                  >  2:698369/1‑140 (MQ=255)                                                           aaCTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTaa                                                                              >  2:1125799/1‑140 (MQ=255)                                                              tCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTg                                                                           <  2:969679/140‑1 (MQ=255)                                                                 ttGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGgcg                                                                        <  1:464618/140‑1 (MQ=255)                                                                 ttGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGgcg                                                                        <  1:683493/140‑1 (MQ=255)                                                                  tgatgaCCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGgcgc                                                                       >  1:1035892/1‑140 (MQ=255)                                                                     tgaCCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCa                                                                    >  2:658247/1‑140 (MQ=255)                                                                       aCCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTg                                                                           <  1:987532/131‑1 (MQ=255)                                                                       aCCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTg                                                                           >  2:987532/1‑131 (MQ=255)                                                                       aCCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAAt                                                                  >  2:1038226/1‑140 (MQ=255)                                                                       aCCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAAt                                                                  >  1:884661/1‑140 (MQ=255)                                                                        ccTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATa                                                                 >  2:254907/1‑140 (MQ=255)                                                                          ttttCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGt                                                               >  2:364023/1‑140 (MQ=255)                                                                           tttCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTg                                                              <  1:1231953/140‑1 (MQ=255)                                                                            ttcttcGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGc                                                             >  2:679185/1‑140 (MQ=255)                                                                            ttcttcGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGc                                                             >  2:21944/1‑140 (MQ=255)                                                                                  gcgATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTc                                                       >  2:689614/1‑140 (MQ=255)                                                                                   cgATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTcc                                                      >  2:387335/1‑140 (MQ=255)                                                                                   cgATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTcc                                                      >  1:148892/1‑140 (MQ=255)                                                                                   cgATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTcc                                                      >  2:566787/1‑140 (MQ=255)                                                                                   cgATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTcc                                                      >  2:374745/1‑140 (MQ=255)                                                                                     aTCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCgg                                                    <  1:254907/140‑1 (MQ=255)                                                                                      tCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGc                                                   <  2:161502/140‑1 (MQ=255)                                                                                            aaGCCTTCTTCGCCTTCTAAATCGAAAACCCAGGCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCAt                                             <  2:544645/140‑1 (MQ=255)                                                                                                 ttcttcGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGcc                                        <  1:70609/140‑1 (MQ=255)                                                                                                 ttcttcGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGcc                                        <  1:430912/140‑1 (MQ=255)                                                                                                 ttcttcGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCCTTTTCCGGCACCCATAGGcc                                        <  1:949673/140‑1 (MQ=255)                                                                                                   cttcGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCa                                      <  2:400152/140‑1 (MQ=255)                                                                                                   cttcGCCTTCTAAATCGAAAACCCAGCCAACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCa                                      >  1:743219/1‑140 (MQ=255)                                                                                                    ttcGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCaa                                     <  2:247107/140‑1 (MQ=255)                                                                                                      cGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAAcc                                   >  1:786958/1‑140 (MQ=255)                                                                                                         cTTCTAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAACCAGa                                <  1:526143/140‑1 (MQ=255)                                                                                                             tAAATCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAACCAGAAAGa                            >  1:963619/1‑140 (MQ=255)                                                                                                              aaaTCGAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAACCAGAAAGat                           >  1:398971/1‑140 (MQ=255)                                                                                                                   gAAAACCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAACCAGAAAGATATTGc                      <  1:698369/140‑1 (MQ=255)                                                                                                                    aaaaCCCAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAACCAGAAAGATATTGCg                     >  1:1256898/1‑140 (MQ=255)                                                                                                                         ccAGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAACCAGAAAGATATTGCGTTtct                >  1:956993/1‑140 (MQ=255)                                                                                                                           aGCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAACCAGAAAGATATTGCGTTTctct              >  2:1062384/1‑140 (MQ=255)                                                                                                                           aGCCCACATCAGCTCAGGTTCGTTTCTCAATATCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAACCAGAAAGATATTGCg                     <  2:670427/133‑1 (MQ=255)                                                                                                                           aGCCCACATCAGCTCAGGTTCGTTTCTCAATATCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAACCAGAAAGATATTGCg                     >  1:670427/1‑133 (MQ=255)                                                                                                                            gCCCACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAACCAGAAAGATATTGCGTTTCTCTg             >  1:1283676/1‑140 (MQ=255)                                                                                                                             cccACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAACCAGAAAGATATTGCGTTTCTCTGc            <  1:1038226/140‑1 (MQ=255)                                                                                                                              ccACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAACCAGAAAGATATTGCGTTTCTCTGcc           <  1:564942/140‑1 (MQ=255)                                                                                                                              ccACATCAGCTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAACCAGAAAGATATTGCGTTTCTCTGcc           <  1:1044018/140‑1 (MQ=255)                                                                                                                                       cTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAACCAGAAAGATATTGCGTTTCTCTGCCATTTTTTCg  >  2:1289431/1‑140 (MQ=255)                                                                                                                                       cTCAGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAACCAGAAAGATATTGCGTTTCTCTGCCATTTTTTCg  >  1:529949/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         TTCACGGGATTTCACAGAGCCGCCGCCAGTAGCCAGCACAATACCCTGTTTCTCGGTCAACTCATTGATGACCTTTTCTTCGCGATCGCGGAAGCCTTCTTCGCCTTCTAAATCGAAAACCCAGCCCACATCAGCTCCGGTTCGTTTCTCAATCTCTTGATCGGAATCGTAAAATTCCATATTGAGTTGTTGAGCTAACTGGCGCCCAATAGTGCTTTTTCCGGCACCCATAGGCCCAACCAGAAAGATATTGCGTTTCTCTGCCATTTTTTCG  >  NC_000913/3518798‑3519071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑