Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,678,114	G→T	H79Q (CAC→CAA)	yhjG ←	AsmA family protein YhjG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,678,114	0	G	T	100.0%	235.0 / NA	67	H79Q (CAC→CAA)	yhjG	AsmA family protein YhjG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/38);  total (29/38)

TTTCGAGCTTGATCCACGGCAGCCAGACCGTTTTGGTCAGCAGCGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGTTT  >  NC_000913/3677978‑3678248                                                                                                                                         |                                                                                                                                       tttCGAGCTTGATCCACGGCAGCCAGACCGTTTTGGTCAGCAGCGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCttgt                                                                                                                                     >  2:150469/1‑140 (MQ=255)  ttCGAGCTTGATCCACGGCAGCCAGACCGTTTTGGTCAGCAGCGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTa                                                                                                                                    <  1:457730/140‑1 (MQ=255)                 cGGCAGCCAGACCGTTTTGGTCAGCAGCGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCa                                                                                                                     >  1:288769/1‑140 (MQ=255)                  ggCAGCCAGACCGTTTTGGTCAGCAGCGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCAc                                                                                                                    >  2:826189/1‑140 (MQ=255)                   gCAGCCAGACCGTTTTGGTCAGCAGCGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCAcc                                                                                                                   >  2:1239538/1‑140 (MQ=255)                     aGCCAGACCGTTTTGGTCAGCAGCGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCa                                                                                                                 >  1:220523/1‑140 (MQ=255)                      gCCAGACCGTTTTGGTCAGCAGCGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAg                                                                                                                >  2:60829/1‑140 (MQ=255)                       ccAGACCGTTTTGGTCAGCAGCGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGc                                                                                                               >  2:607903/1‑140 (MQ=255)                       ccAGACCGTTTTGGTCAGCAGCGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGc                                                                                                               >  2:52882/1‑140 (MQ=255)                       ccAGACCGTTTTGGTCAGCAGCGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGc                                                                                                               >  2:1306233/1‑140 (MQ=255)                       ccAGACCGTTTTGGTCAGCAGCGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGc                                                                                                               >  2:453141/1‑140 (MQ=255)                           aCCGTTTTGGTCAGCAGCGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTgcg                                                                                                           <  1:1306233/140‑1 (MQ=255)                           aCCGTTTTGGTCAGCAGCGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTgcg                                                                                                           <  1:60829/140‑1 (MQ=255)                                tttGGTCAGCAGCGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCgccagc                                                                                                      >  1:536079/1‑140 (MQ=255)                                      cagcagCGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGttt                                                                                                >  2:924568/1‑140 (MQ=255)                                        gcagcGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCt                                                                                              <  1:146627/140‑1 (MQ=255)                                            cgcCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGtt                                                                                          >  1:657932/1‑140 (MQ=255)                                             gcCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGttt                                                                                         <  2:897575/140‑1 (MQ=255)                                              ccAGCGGGGCCAGCGCTGCCTGTCCGCGTAGCAAATGCACCATCGTGACTTCGGGAATATCCGGAGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGCCCACGGCACCCAGCTGCGCCAGCCAGCTTCTTgatct                                                                                        >  2:1277916/1‑136 (MQ=255)                                                 gCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGc                                                                                     <  2:1048763/140‑1 (MQ=255)                                                 gCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGc                                                                                     <  2:918696/140‑1 (MQ=255)                                                 gCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGc                                                                                     <  2:220523/140‑1 (MQ=255)                                                 gCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGc                                                                                     <  1:343944/140‑1 (MQ=255)                                                   ggggCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCg                                                                                   <  1:685877/140‑1 (MQ=255)                                                      gCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTc                                                                                <  1:239747/140‑1 (MQ=255)                                                       ccAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTcc                                                                               <  2:1178755/140‑1 (MQ=255)                                                       ccAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTcc                                                                               <  2:457475/140‑1 (MQ=255)                                                           cGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCcaca                                                                           <  1:980215/140‑1 (MQ=255)                                                            gTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCacac                                                                          <  2:536079/140‑1 (MQ=255)                                                             ttGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCAcacc                                                                         <  1:1310346/140‑1 (MQ=255)                                                             ttGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCAcacc                                                                         >  1:749602/1‑140 (MQ=255)                                                               gCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCAcaccac                                                                       >  1:317801/1‑140 (MQ=255)                                                               gCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCAcaccac                                                                       >  2:1028259/1‑140 (MQ=255)                                                                        cgTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATc                                                              <  1:1226201/140‑1 (MQ=255)                                                                          tGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGc                                                            >  2:385256/1‑140 (MQ=255)                                                                          tGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGc                                                            <  1:638717/140‑1 (MQ=255)                                                                          tGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGc                                                            <  1:533386/140‑1 (MQ=255)                                                                           ggCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGcc                                                           >  1:1095183/1‑140 (MQ=255)                                                                           ggCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGcc                                                           <  2:657932/140‑1 (MQ=255)                                                                           ggCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGAGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGccc                                                                   <  1:245323/132‑1 (MQ=255)                                                                           ggCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGAGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGccc                                                                   >  2:245323/1‑132 (MQ=255)                                                                             cAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCAc                                                         <  2:288769/140‑1 (MQ=255)                                                                                       aTCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGaa                                               >  2:503001/1‑140 (MQ=255)                                                                                         cGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACg                                             <  1:1239538/140‑1 (MQ=255)                                                                                         cGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACg                                             <  2:262722/140‑1 (MQ=255)                                                                                          gTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACgg                                            >  2:769550/1‑140 (MQ=255)                                                                                                tCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGAtt                                      <  1:385256/140‑1 (MQ=255)                                                                                                 cGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTc                                     >  1:389410/1‑140 (MQ=255)                                                                                                  gggAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCa                                    >  2:181621/1‑140 (MQ=255)                                                                                                    gAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAAc                                  <  2:761759/140‑1 (MQ=255)                                                                                                        atCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCg                              <  1:503001/140‑1 (MQ=255)                                                                                                         tCCGGTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGc                             >  1:157490/1‑140 (MQ=255)                                                                                                            ggTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCaga                          <  1:1248238/140‑1 (MQ=255)                                                                                                            ggTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCaga                          <  1:826189/140‑1 (MQ=255)                                                                                                             gTGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCTGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCagag                         <  2:941413/140‑1 (MQ=255)                                                                                                              tGGATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAgaga                        <  2:222276/140‑1 (MQ=255)                                                                                                                gATTGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACt                      <  2:598297/140‑1 (MQ=255)                                                                                                                  ttGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACttt                    <  2:80748/140‑1 (MQ=255)                                                                                                                   tGCCAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTc                   >  2:1204182/1‑140 (MQ=255)                                                                                                                      cAAGAATGATGTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTATCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTgg                <  1:1167939/140‑1 (MQ=255)                                                                                                                              atgTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGt        <  2:317801/140‑1 (MQ=255)                                                                                                                              atgTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCAGCAGAGACTTTCTGGTTGATAGt        <  2:980554/140‑1 (MQ=255)                                                                                                                               tgTCTTCCGCTTGTACATGGGGCCACGGCCCCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCCCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTc       >  2:963310/1‑140 (MQ=255)                                                                                                                               tgTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTc       <  1:380309/140‑1 (MQ=255)                                                                                                                               tgTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTc       >  1:379291/1‑140 (MQ=255)                                                                                                                               tgTCTTCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCAACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTc       >  1:642547/1‑140 (MQ=255)                                                                                                                                   ttCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGtt   <  1:648635/140‑1 (MQ=255)                                                                                                                                   ttCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGtt   >  1:5077/1‑140 (MQ=255)                                                                                                                                    tCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGttt  <  2:749602/140‑1 (MQ=255)                                                                                                                                    tCCGCTTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGttt  >  1:1251527/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TTTCGAGCTTGATCCACGGCAGCCAGACCGTTTTGGTCAGCAGCGCCAGCGGGGCCAGCGTTGCCTCTACGCGTGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGTTT  >  NC_000913/3677978‑3678248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑