| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,083,463 | G→T | P787Q (CCG→CAG) | fdoG ← | formate dehydrogenase O subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,083,463 | 0 | G | T | 97.8% | 157.4 / ‑2.7 | 46 | P787Q (CCG→CAG) | fdoG | formate dehydrogenase O subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (19/26); total (19/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑TTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTA > NC_000913/4083328‑4083597 | ccTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCNCGGGTTACCCTGCTGATc < 1:358495/140‑1 (MQ=255) ccTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCg > 2:305959/1‑140 (MQ=255) ccTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGCAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCg > 1:697289/1‑140 (MQ=255) ctgcATGATAAACGGCCCGACGCCGCTCCCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAgg < 2:1083637/140‑1 (MQ=255) gcATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGgcg < 1:883582/140‑1 (MQ=255) gcATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGgcg < 2:1010972/140‑1 (MQ=255) gcATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGgcg < 2:451701/140‑1 (MQ=255) gcATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGgcg < 1:669215/140‑1 (MQ=255) gcATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGgcg < 1:656194/140‑1 (MQ=255) gcATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGgcg < 2:224332/140‑1 (MQ=255) cATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGgcgc > 2:183683/1‑140 (MQ=255) tGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCgg > 1:268068/1‑140 (MQ=255) aTAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGtt < 1:305959/140‑1 (MQ=255) ccGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAAt > 2:14777/1‑140 (MQ=255) aCGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATgcg < 2:524115/140‑1 (MQ=255) aCCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTaa < 1:494815/140‑1 (MQ=255) cccGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAg < 2:268068/140‑1 (MQ=255) cccGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAg < 1:1214545/140‑1 (MQ=255) aGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGcc < 1:519886/140‑1 (MQ=255) aGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAACGGcc > 1:219961/1‑140 (MQ=255) ggCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGcca < 1:1307019/140‑1 (MQ=255) gcgctgcgctGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGccat > 2:1109563/1‑140 (MQ=255) tgcgctgTAGTTCGTAATACCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGccatgtcc < 2:83500/140‑1 (MQ=255) tgTAGTCCGGAATATCCCAGTCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCcatgcccatgcc < 1:1065859/140‑1 (MQ=255) tatCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCgtg < 2:286992/140‑1 (MQ=255) tatCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCgtg > 2:1251913/1‑140 (MQ=255) tCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑tt > 1:933213/1‑140 (MQ=255) tCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑tt > 1:243597/1‑140 (MQ=255) ccGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCCTGCCCAGCCCAGCGTG‑TTACCGAg > 2:617705/1‑140 (MQ=255) ggTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑TTACCGAGGc > 1:564357/1‑140 (MQ=255) cACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑TTACCGAGGCCAGa < 1:183683/140‑1 (MQ=255) gTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCTCGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGAGGTTAAGCGGCCATGCCCATAGCCAGCGCAGCGTG‑TTACCGAGGGCAGCCGGAtg < 2:697289/140‑2 (MQ=255) gTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑TTACCGAGGCCAGACGGATc < 1:92005/140‑1 (MQ=255) gTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑TTACCGAGGCCAGACGGATc < 2:571877/140‑1 (MQ=255) tCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGGCCTGCCCATCCCCAGCCCCGCGTG‑TTTCCGAGGCCAGACGGATAGGCGtt > 1:287937/1‑140 (MQ=255) tCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑TTACCGAGGCCAGACGGATCgg > 1:74428/1‑136 (MQ=255) tCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑TTACCGAGGCCAGACGGATCgg < 2:74428/136‑1 (MQ=255) tCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑TTACCGAGGCCAGACGGATCGGCGtt > 1:429643/1‑140 (MQ=255) tCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTGAAGCCGCCATGCCCCTTCCCAGCCCAGCGTG‑TTACCGAGGCCAGACGGATCGGCGtt > 2:772796/1‑140 (MQ=255) cATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑TTACCGAGGCCAGACGGATCGGCGTTATc < 1:14777/140‑1 (MQ=255) ttCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑TTACCGAGGCCAGACGGATCGGCGTTATCACg < 2:995292/140‑1 (MQ=255) tCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑TTACCGAGGCCAGACGGATCGGCGTTATCACgg < 1:1013683/140‑1 (MQ=255) gCAACTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑TTACCGAGGCCAGACGGATCGGCGTTATCACGGCGt > 2:1084870/1‑140 (MQ=255) aaCTGACGCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCCACCATGCCCATTCCCCCCCCCCCGTGTTTACCG‑GGGCAAACGGGTACGCGTTTTTACGGCGTGc > 2:650997/1‑140 (MQ=255) gCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑TTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTgg < 1:1109563/140‑1 (MQ=255) gCTTCGGATCCCACGGGTTACCCTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑TTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTgg < 1:1140581/140‑1 (MQ=255) ccTGCTGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑TTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTa > 1:705350/1‑140 (MQ=255) | CCTTCCTGCTGCATGATAAACGGCCCGACGCCGCTACCCGGAGGCGCTGCGCTGTAGTCCGGAATATCCCAGCCGGTCCACTTAGTGCCGTCCCATTTCAGCAACTGACGCTTCGGATCCCACGGGTTACCCTGCGGATCTGCGGAGGCGCGGTTATACAGAATGCGGCGGTTAAGCGGCCATGCCCATGCCCAGCCCAGCGTG‑TTACCGAGGCCAGACGGATCGGCGTTATCACGGCGTGCCATCTGGTTGCCTTCCGGCGTCCAGCTA > NC_000913/4083328‑4083597 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |