Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913445,5580AC28.8% 103.2 / 36.8 66V370G (GTA→GGA) yajRputative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (39/8);  new base C (0/19);  total (39/27)
Fisher's exact test for biased strand distribution p-value = 1.28e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 101 total aligned reads displayed.

AGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGT  >  NC_000913/445420‑445692
                                                                                                                                          |                                                                                                                                         
aGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGGACTGGCGACTGTCAGCCACACCGCGGCCAGCATTGCGCCCGCGAGAAAAAc                                                                                                                                          >  2:438964/1‑140 (MQ=255)
   aaaCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATAcccc                                                                                                                                       >  1:136310/1‑140 (MQ=255)
     aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                     <  2:796325/140‑1 (MQ=255)
     aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                     <  2:2240301/140‑1 (MQ=255)
     aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                     <  2:1234157/140‑1 (MQ=255)
     aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                     <  1:192561/140‑1 (MQ=255)
      cGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTg                                                                                                                                    <  1:438964/140‑1 (MQ=255)
       gCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGa                                                                                                                                   <  1:2232594/140‑1 (MQ=255)
                aCGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCCGCGAGAAAAACCCCCCGACCGTCaaaa                                                                                                                          >  2:110909/1‑139 (MQ=255)
                   ccTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCACACTTCGGCCAGCATTTCGCCCGCGGGAAATACCCCCCGACCGTCAAACATg                                                                                                                       >  2:577502/1‑140 (MQ=255)
                                 aTGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAgcc                                                                                                         <  1:346696/140‑1 (MQ=255)
                                                        cAAACTCCTGACATACGGGGGTTCTTTCAGGGTACTGGCGACTGTCAGCCACACTGCGGCCAGAATTGCGCCAGCGAAAAATCCCCCCTGCCCGCAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgcca                                                                                  <  2:1400284/140‑1 (MQ=255)
                                                            ctgcggAAATAGGGGGGTTCTTTCAGGGTCCGGGCGCTTGTGAGCCACACGGGGGCCAGCATGGCGCAAGGGAGAAATACCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgccacgcc                                                                              <  2:1628470/140‑1 (MQ=255)
                                                                  aCATACGGGGGTTCTTTCAGGGTACGGCCGACTGCCAGCCACACGGGGCCCAGCATTGCGCCAGGAAAAAACCCCCCCTCCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGaaa                                                                        <  2:1441026/140‑1 (MQ=255)
                                                                    aTAGGGGGGTTTTTTAATGGTACGGGCGCCTGTCCCCCACACGGCGGCCGGCTTGGCGCACGGGAGAAATCCCCCCTTACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑ct                                                                   <  2:591489/140‑1 (MQ=255)
                                                                       cggcggTTTTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑ctggc                                                                <  2:136310/140‑1 (MQ=255)
                                                                       cggcggTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCCAGAAA‑‑‑ctggc                                                                >  1:287623/1‑140 (MQ=255)
                                                                       cggcggTTCTTTCATGGTACTGGCGACTGTCAGCCACACCGCGGCCAGCAATGCGCCAGCGAGAAATACCCCCCGACCGGCAAACAAGCCGTTTATCCAACCCCCCCGCGAAACGCCCAACGCCACGCCAAGAAA‑‑‑ctggc                                                                >  2:747081/1‑140 (MQ=255)
                                                                          gggTTCTTTAAGGGTACTGGCGACTGTCAGCCACACGGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑ctggctgg                                                             <  2:2023769/139‑1 (MQ=255)
                                                                           ggTTCTTTCAGGGTACTGGCGACTGTCACCCACACTGCGGCAAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCtggt                                                            <  2:1255125/140‑1 (MQ=255)
                                                                            gTTTTTTTATGGTACTGGGGGCTGTCAGCCACACTCCGGCCCGCATTGCGGCAGCGAGAAAAACCCCCCGACCGTCAAACATGCCGTTAATCCCACCCCCCAGCGAACCGCCAATCGCCCCGCCAAGAAA‑‑‑atggccaggg                                                           >  2:1289751/1‑135 (MQ=255)
                                                                            gTTCTTTCATGGTCCTGGCGCCTGTCGGCCCCACTGGGGCCAGAATTGCGCCAGGGAGAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCtggtg                                                           <  2:1285301/140‑1 (MQ=255)
                                                                            gTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAAAACCCCCCGACCGTCAAACATGCCGTTAATCCAGCCGCCCCGCGAACCGCCAAACGCCCCGCCCAGAAA‑‑‑CTGGCtggtg                                                           >  2:441150/1‑140 (MQ=255)
                                                                              tCTTTTATGGTACTGGCGACTGTCAGCCACACTGCGGCCCGCATTGCGCCCGCGAGAAAAACCCCCCGACCCTCAAACATGCCCTTAATCCAGCCCCCCCGCGAACCCCCCATCGCCACGCCCAGAAA‑‑‑ACGGCtgggggg                                                         >  2:718250/1‑139 (MQ=255)
                                                                              tCTTTCATGGTACTGGCGACTGTCAGCCACACTGTGGCCAGCATTGCGCCCGCGAGAAAAACCCCCCGACCGTCAAACATGCCGTTAATCCAGCCCCCCAGCGAACCGCCAATCCCCCCCCCAAGAAA‑‑‑CTGGCtggtggg                                                         >  2:369016/1‑139 (MQ=255)
                                                                                   cATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTaaa                                                    <  1:2028731/140‑1 (MQ=255)
                                                                                    aTGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCCTTTCGCCAGCGAGAAAAACCCCCTGACCGTCAAACATGCCGGTAATCCAGCCGCCCAGCGAACCGCCCAACGCCACGCCCAGAAA‑‑‑ATGGCCGGGGGAGGaaaa                                                   >  2:1688351/1‑139 (MQ=255)
                                                                                     tGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAaca                                                  >  1:389733/1‑140 (MQ=255)
                                                                                       gTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACAcc                                                >  1:1392650/1‑140 (MQ=255)
                                                                                       gTACTGGCGACTGTCACCCACACTGCGGCCAGCATTGCGCCGGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACAcc                                                <  1:710470/140‑1 (MQ=255)
                                                                                        tACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGCAAACAccc                                               <  1:1404500/140‑1 (MQ=255)
                                                                                         aCTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCa                                              >  2:612837/1‑140 (MQ=255)
                                                                                          cTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCAt                                             >  2:1981564/1‑140 (MQ=255)
                                                                                           tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATc                                            >  1:1700651/1‑140 (MQ=255)
                                                                                           tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATc                                            >  1:64469/1‑140 (MQ=255)
                                                                                           tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATc                                            >  1:648642/1‑140 (MQ=255)
                                                                                           tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATc                                            >  1:2050742/1‑140 (MQ=255)
                                                                                            ggCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCg                                           <  1:747081/140‑1 (MQ=255)
                                                                                            ggCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCg                                           >  2:1699846/1‑140 (MQ=255)
                                                                                             gCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGc                                          >  1:2225838/1‑140 (MQ=255)
                                                                                              cGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGTAGTAAACACCCATCGcc                                         >  1:460292/1‑140 (MQ=255)
                                                                                              cGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGcc                                         >  1:1415396/1‑140 (MQ=255)
                                                                                               gACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCg                                        <  1:22517/140‑1 (MQ=255)
                                                                                               gACTGTCACCCACCCGGGGGCAAGCATTGCGCCAGGGAAAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCACCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCg                                        <  2:2146927/140‑1 (MQ=255)
                                                                                                aCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGt                                       >  1:140743/1‑140 (MQ=255)
                                                                                                 cTGCCAGCCCCCCTGCGCCCAGCATTGCCCCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTa                                      <  2:58149/140‑1 (MQ=255)
                                                                                                 cTGCCACCCACACTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTa                                      <  2:1982031/140‑1 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTa                                      <  1:135902/139‑1 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTa                                      >  2:135902/1‑139 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                     >  2:1710103/1‑140 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                     >  2:1420987/1‑140 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                     >  1:58149/1‑140 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                     >  2:440984/1‑140 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                     >  1:1982031/1‑140 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                     >  1:2084359/1‑140 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGGGGGGTAAACACCCATCCCCGTAc                                     >  2:1852555/1‑140 (MQ=255)
                                                                                                      aGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCttt                                 >  1:1458017/1‑140 (MQ=255)
                                                                                                       gCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGCTGGCTGGTGGAGTAAACACCCATCGCCGTACCt                                   >  1:31097/1‑140 (MQ=255)
                                                                                                       gCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTg                                >  1:832805/1‑140 (MQ=255)
                                                                                                       gCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTg                                >  1:1893913/1‑140 (MQ=255)
                                                                                                              tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                         >  2:236252/1‑140 (MQ=255)
                                                                                                              tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACACATCGCCGTACCTTTGTAACctg                         >  1:15403/1‑140 (MQ=255)
                                                                                                              ggCGGCCAGCATTGCCCCAGCGAAAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                         <  2:140743/139‑1 (MQ=255)
                                                                                                                cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                       >  2:368569/1‑140 (MQ=255)
                                                                                                                 ggCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgctg                      >  1:599648/1‑140 (MQ=255)
                                                                                                                  gcaaGCATTGCGCCGGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                     <  1:486606/137‑1 (MQ=255)
                                                                                                                  gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAAccctgtct                     >  1:648958/1‑134 (MQ=255)
                                                                                                                  gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                     <  1:236252/140‑1 (MQ=255)
                                                                                                                  gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                     >  2:206023/1‑140 (MQ=255)
                                                                                                                    cAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCg                   <  1:612837/140‑1 (MQ=255)
                                                                                                                     agaatggcgcCGGCGAAAAATCCCCCCTGCCCTCAAAACATGCCGTTAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                  <  2:701333/134‑1 (MQ=255)
                                                                                                                     aGCTTTGCCCCAGCGAGAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                  <  1:1289751/140‑1 (MQ=255)
                                                                                                                      gCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAc                 >  1:1413264/1‑140 (MQ=255)
                                                                                                                       ctttGCGCCGGGGAAAAACCCCCCCTGCCCGCAAAACATGCCGTTAATCCACCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                <  2:1341896/138‑1 (MQ=255)
                                                                                                                       cATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                <  1:1981564/140‑1 (MQ=255)
                                                                                                                       cATTGCCCCAGCGAAAAATCCCCCCGGACCGTAAACCATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                <  2:2050742/140‑1 (MQ=255)
                                                                                                                       cATCGCCCCAGCGAGAAATCCCCCCTGCCCGCAAAACATGCCGTTAATCCAGCCGCCCAGCGACCCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                <  2:2084359/140‑1 (MQ=255)
                                                                                                                        ttgccgcCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGCTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                  <  2:31097/136‑1 (MQ=255)
                                                                                                                         ttGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCGGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct              <  1:620360/140‑1 (MQ=255)
                                                                                                                         ttGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct              <  1:399352/140‑1 (MQ=255)
                                                                                                                         ttGCGCCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct              <  2:287623/140‑1 (MQ=255)
                                                                                                                         ttGCGCCAGCAAGAATTCCCCCCTGACCGTCAAACATGCCGTTAACCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct              <  1:105698/140‑1 (MQ=255)
                                                                                                                          tGCGCCAGCGAGAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCtt             <  1:110909/140‑1 (MQ=255)
                                                                                                                           gggcCAGGGAAAATTCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt            <  1:1242210/138‑1 (MQ=255)
                                                                                                                           gcgcCAGCGAGAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt            <  1:441150/140‑1 (MQ=255)
                                                                                                                           gcgcCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt            <  1:718250/140‑1 (MQ=255)
                                                                                                                           gcgcCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt            <  1:1688351/140‑1 (MQ=255)
                                                                                                                           gcgcCAGCGAAAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt            <  1:369016/140‑1 (MQ=255)
                                                                                                                            cgcCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa           <  1:2134684/140‑1 (MQ=255)
                                                                                                                            cgcCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa           >  1:1002059/1‑140 (MQ=255)
                                                                                                                            cgcCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa           >  1:523623/1‑140 (MQ=255)
                                                                                                                            cgcCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTa           >  1:2231473/1‑140 (MQ=255)
                                                                                                                                ggCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa       <  2:648642/139‑1 (MQ=255)
                                                                                                                                agagagaAACCCCCCCTGCCCTTCAACAACGCCGTAAACCCCGCCGCCCAGGGACCCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa       <  2:832805/137‑1 (MQ=255)
                                                                                                                                aGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa       <  1:864472/140‑1 (MQ=255)
                                                                                                                                aGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa       <  2:1893913/140‑1 (MQ=255)
                                                                                                                                aGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa       <  2:599648/140‑1 (MQ=255)
                                                                                                                                 gCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt      >  2:347020/1‑140 (MQ=255)
                                                                                                                                   gagaAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATaa    <  1:1420987/140‑1 (MQ=255)
                                                                                                                                     aaaaTCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACACATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt  <  2:15403/139‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
AGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAA‑‑‑CTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGT  >  NC_000913/445420‑445692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑