Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,337,712	G→T	A570E (GCA→GAA)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,337,712	0	G	T	100.0%	103.5 / NA	30	A570E (GCA→GAA)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/1);  total (29/1)

CGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGG  >  NC_000913/2337575‑2337834                                                                                                                                          |                                                                                                                           cgcgcgcgCCACGAGTGGCTTTCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGCGAGGGTTCGCCACCAGCAGCCGGTCGATAAAGCCTTCTTCTTTAATACGTTCg                                                                                                                          >  2:1440059/1‑140 (MQ=255)  gcgcgcgcCACGAGTGGCTTTCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCgg                                                                                                                         <  1:1440059/140‑1 (MQ=255)   cgcgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGc                                                                                                                        >  1:915147/1‑140 (MQ=255)     cgcgcCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAACACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAg                                                                                                                      >  1:1170836/1‑140 (MQ=255)          cACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTa                                                                                                                 >  1:725010/1‑140 (MQ=255)          cACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTa                                                                                                                 >  1:1783533/1‑140 (MQ=255)                         gCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCacga                                                                                                  >  1:1242577/1‑140 (MQ=255)                            aCTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGc                                                                                               >  1:684208/1‑140 (MQ=255)                                aTAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTgcg                                                                                           >  1:1659808/1‑140 (MQ=255)                                aTAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTgcg                                                                                           >  1:572275/1‑140 (MQ=255)                                        tCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCatat                                                                                   >  2:1729702/1‑140 (MQ=255)                                               aTAGCCGCGACCCCGGGGGGAAAACCACAAAATATGGTCGTGAGTGTTCCCCACCAGCGGTCGGTCGATAAATTCTTCTTCTTTAATACTTTGGGAAGTTTTCCTTTTCCCGCCACGACGCTGCGCTTCGTATTCAGaaa                                                                            <  2:24561/140‑1 (MQ=255)                                                tAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAg                                                                           >  1:879716/1‑140 (MQ=255)                                                 aGACGCGCCCCGGGCGGGAGAAGCAAAGAATATGGTCGTGAGTTTCCCCCCCCAGCAGTCGGTCGATAAATTCTTTTTTTTTAATCCGTCCGGCAGTTTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGc                                                                          <  2:1642554/140‑1 (MQ=255)                                                  gACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCg                                                                         >  1:1611400/1‑140 (MQ=255)                                                   aCGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATATGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCgg                                                                        >  1:264001/1‑140 (MQ=255)                                                    cgcgACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGc                                                                       >  1:1293119/1‑140 (MQ=255)                                                      cgcccACGGCTGAAGAAGCACAGAATATGGTCGTGAGTGTCCGCCACAAGCAGCCGTTCGATAAATTTTTCTTCTTAAATACGTTGGAAAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTg                                                                     <  2:1659808/137‑1 (MQ=255)                                                          cACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATAc                                                                 >  1:1542721/1‑140 (MQ=255)                                                           ccGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTCCCCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACt                                                                <  2:1242577/139‑1 (MQ=255)                                                                gggAAAAGCACATAATATGGTGGTGGGTTTCCCCCCCCAGCAGTCGGTAGATAAATTCTTCTTTTTTAATGCGTTGGGCAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACg                                                           <  2:1293119/139‑1 (MQ=255)                                                                  gagaAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTa                                                         >  1:1845828/1‑140 (MQ=255)                                                                  gagaAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTa                                                         >  1:1613872/1‑140 (MQ=255)                                                                     aaGCAAAAAATATGGTCGTGAGTTTCCCCCACCAGCAGTCGGTCAATAAAGTCTTCTTTTTTAATACGTTCGGCAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGcc                                                      <  2:1976234/140‑1 (MQ=255)                                                                       gCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCt                                                    >  1:1550990/1‑140 (MQ=255)                                                                             aaTATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgag                                              >  2:1915336/1‑140 (MQ=255)                                                                               tatGGTGGTGAGTGTTCCCCCCCAGCAGTCGGCCGATAAAGTTTTTTTCTTTAATACGTTCGGAAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgagag                                            <  2:1730849/140‑1 (MQ=255)                                                                                    tggTGAGTTTTCGCCCCCAGCAGTCGGTCAATAAAGTCTTTTTTTTTAATACTTTCGGCAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGt                                       <  2:725010/138‑1 (MQ=255)                                                                                    tCGGGAGTGTCCCCCACCAGCAGTCGGTCAATAAAGTTTTTTTCTTTAATCCGTTCGGAAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGt                                       <  2:388209/140‑1 (MQ=255)                                                                                       tGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCAc                                    >  2:62980/1‑140 (MQ=255)                                                                                       tGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCAc                                    >  1:1875230/1‑140 (MQ=255)                                                                                       tGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCAc                                    >  2:1697315/1‑140 (MQ=255)                                                                                       tGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCAc                                    >  1:678371/1‑140 (MQ=255)                                                                                       tGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCAc                                    >  1:602814/1‑140 (MQ=255)                                                                                       tGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCAc                                    >  1:388209/1‑140 (MQ=255)                                                                                             tcccccaccaGCAGTCGGTCGATAAAGTCTTTTTCTTTAATCCGTTCGGCAGATTTCCTTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACcac                              <  2:678371/136‑1 (MQ=255)                                                                                             tccGCCACCAGCAGTCGGTCAATAAAGTCTTCTTTTTTAATCCGTTCGGCAGTTTTCCTTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACcac                              <  2:572275/138‑1 (MQ=255)                                                                                                  ccccaGCAGTCGGTCGATAAAGTTTTTTTTTTAAATACGTTGGGCAGTTTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt                         <  2:1613872/138‑1 (MQ=255)                                                                                                  caccaGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCtt                         >  2:338248/1‑140 (MQ=255)                                                                                                    ccaGCTGTCGGTCAAAAAAGTCTTCTTCTTTAATACTTCCGGAAGATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTcc                       <  2:602814/140‑1 (MQ=255)                                                                                                     cagcagTCGGTCGATAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCt                      >  1:1639292/1‑140 (MQ=255)                                                                                                                  aTAAAGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATc         >  1:58153/1‑140 (MQ=255)                                                                                                                    aaaTTCTTCTTCTTTAATACGTCCGGAAAATTTCCCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCtt       <  2:915147/140‑1 (MQ=255)                                                                                                                    aaaGTCTTCTTTTTAAATCCGTCCGGCAGATTTCCTTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCtt       <  2:58153/140‑1 (MQ=255)                                                                                                                     aaGTCTTCTTCTTTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTc      >  1:1728009/1‑140 (MQ=255)                                                                                                                      aGTCTTCTTCTTTAATACGTTCGGCAGATTTCCCTTCCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTcc     <  1:1689620/140‑1 (MQ=255)                                                                                                                         cttcttcttTAATACGTTCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAgg  >  2:1733382/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                           CGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTTCCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGG  >  NC_000913/2337575‑2337834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑