Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,847,744	G→A	Q500* (CAA→TAA)	hycC ←	formate hydrogenlyase subunit HycC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,847,744	0	G	A	100.0%	337.1 / NA	94	Q500* (CAA→TAA)	hycC	formate hydrogenlyase subunit HycC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/49);  total (45/49)

CATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGCCACTACGCTTACGCTCATCAGGAGCG  >  NC_000913/2847607‑2847878                                                                                                                                          |                                                                                                                                       cATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTaag                                                                                                                                      >  1:567764/1‑140 (MQ=255)   ttGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGaa                                                                                                                                    >  1:1368789/1‑140 (MQ=255)    tGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGTCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGaaa                                                                                                                                   >  2:1666590/1‑140 (MQ=255)    tGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCGTCGGTTaag                                                                                                                                      >  1:919766/1‑137 (MQ=255)    tGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTaag                                                                                                                                      >  1:55315/1‑137 (MQ=255)    tGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGaaa                                                                                                                                   >  1:1701230/1‑140 (MQ=255)    tGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGaaa                                                                                                                                   >  1:37448/1‑140 (MQ=255)    tGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGaaa                                                                                                                                   >  2:103271/1‑140 (MQ=255)    tGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGaaa                                                                                                                                   >  2:552512/1‑140 (MQ=255)    tGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGaaa                                                                                                                                   >  1:1373028/1‑140 (MQ=255)     gATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAAc                                                                                                                                  <  2:1980697/140‑1 (MQ=255)      aTTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACg                                                                                                                                 <  2:37448/140‑1 (MQ=255)       tttttCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACgg                                                                                                                                <  1:239921/140‑1 (MQ=255)       tttttCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACgg                                                                                                                                <  1:1313892/140‑1 (MQ=255)       tttttCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACgg                                                                                                                                <  2:676143/140‑1 (MQ=255)          ttCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACggtgg                                                                                                                             >  2:2028435/1‑140 (MQ=255)                         gCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCa                                                                                                              <  2:1230170/140‑1 (MQ=255)                         gCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCa                                                                                                              >  2:299025/1‑140 (MQ=255)                          cacaCCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAg                                                                                                             >  2:700347/1‑140 (MQ=255)                          cacaCCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAg                                                                                                             >  1:190269/1‑140 (MQ=255)                           acacCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAg                                                                                                                       <  1:1460046/129‑1 (MQ=255)                           acacCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAg                                                                                                                       >  2:1460046/1‑129 (MQ=255)                              cccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGc                                                                                                         <  2:567764/140‑1 (MQ=255)                              cccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGc                                                                                                         <  2:844182/140‑1 (MQ=255)                              cccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGc                                                                                                         <  1:1268419/140‑1 (MQ=255)                              cccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGc                                                                                                         >  1:8520/1‑140 (MQ=255)                              cccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGc                                                                                                         <  1:159028/140‑1 (MQ=255)                               ccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGc                                                                                                            >  2:300089/1‑136 (MQ=255)                               ccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGc                                                                                                            <  1:300089/136‑1 (MQ=255)                               ccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCgg                                                                                                          >  2:1496625/1‑138 (MQ=255)                               ccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCgg                                                                                                          <  1:1496625/138‑1 (MQ=255)                               ccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCa                                                                                                        <  1:1601325/140‑1 (MQ=255)                               ccAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCa                                                                                                        >  1:411021/1‑140 (MQ=255)                                cAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCag                                                                                                       <  1:2028435/140‑1 (MQ=255)                                 aGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCaga                                                                                                      <  1:299025/140‑1 (MQ=255)                                   gCCGCACCGCGGGAACGCGATGGCAAACGATCGTCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAgg                                                                                                    <  1:1666590/140‑1 (MQ=255)                                      gCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAc                                                                                                 <  1:406070/140‑1 (MQ=255)                                       cACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCGGAGGTAca                                                                                                >  1:1371148/1‑140 (MQ=255)                                       cACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAca                                                                                                >  2:17892/1‑140 (MQ=255)                                       cACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAca                                                                                                >  1:1031402/1‑140 (MQ=255)                                        aCCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcag                                                                                               <  1:1990095/140‑1 (MQ=255)                                          cgcgGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagca                                                                                             <  2:1565727/140‑1 (MQ=255)                                            cgGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcagc                                                                                           >  2:143858/1‑140 (MQ=255)                                             gggAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcagca                                                                                          >  1:128981/1‑140 (MQ=255)                                              ggAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTAcagcagcag                                                                                         <  2:128981/140‑1 (MQ=255)                                                aaCGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCagag                                                                                       <  1:552512/140‑1 (MQ=255)                                                     gATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCAt                                                                                  <  2:1368789/140‑1 (MQ=255)                                                     gATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCAt                                                                                  <  2:8520/140‑1 (MQ=255)                                                      aTGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATc                                                                                 >  1:1912918/1‑140 (MQ=255)                                                       tGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCg                                                                                <  1:665019/140‑1 (MQ=255)                                                       tGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCg                                                                                >  1:606753/1‑140 (MQ=255)                                                          cAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCa                                                                             <  1:740910/140‑1 (MQ=255)                                                          cAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCa                                                                             <  2:411021/140‑1 (MQ=255)                                                          cAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCa                                                                             >  1:1572531/1‑140 (MQ=255)                                                          cAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCa                                                                             <  1:700347/140‑1 (MQ=255)                                                                 tCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCa                                                                      <  2:1373028/140‑1 (MQ=255)                                                                 tCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCa                                                                      <  2:190269/140‑1 (MQ=255)                                                                    ccTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGTAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACgg                                                                   <  2:810733/140‑1 (MQ=255)                                                                    ccTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACgg                                                                   <  2:485520/140‑1 (MQ=255)                                                                        tGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgca                                                               >  1:1561510/1‑140 (MQ=255)                                                                         gCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACgg                                                                   >  2:1906440/1‑135 (MQ=255)                                                                         gCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACgg                                                                   <  1:1906440/135‑1 (MQ=255)                                                                         gCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgcag                                                              >  2:608770/1‑140 (MQ=255)                                                                         gCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCgcag                                                              >  1:44551/1‑140 (MQ=255)                                                                              tCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAaca                                                         <  2:1912918/140‑1 (MQ=255)                                                                               cGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAacac                                                        >  1:1178591/1‑140 (MQ=255)                                                                                  cATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGc                                                     >  2:1593292/1‑140 (MQ=255)                                                                                  cATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGc                                                     >  1:2017552/1‑140 (MQ=255)                                                                                         atgaATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTAc                                              >  2:597320/1‑140 (MQ=255)                                                                                            aaTGGCAGCAGCGGGTAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACgca                                           >  1:2011331/1‑140 (MQ=255)                                                                                            aaTGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCaa                                                           <  1:1163108/124‑1 (MQ=255)                                                                                            aaTGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCaa                                                           >  2:1163108/1‑124 (MQ=255)                                                                                              tGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACgcagc                                         >  1:1096202/1‑140 (MQ=255)                                                                                                gcagcaGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCaa                                       <  1:143858/140‑1 (MQ=255)                                                                                                   gcagcGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATc                                    <  2:664263/140‑1 (MQ=255)                                                                                                   gcagcGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATc                                    >  1:435039/1‑140 (MQ=255)                                                                                                     agcGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGc                                  <  2:1120707/140‑1 (MQ=255)                                                                                                     agcGGGAAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGc                                  <  1:337371/140‑1 (MQ=255)                                                                                                       cGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCa                                >  1:1617809/1‑140 (MQ=255)                                                                                                        gggaagggaaTCAGCAGCAACGTGATCATCGTTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAg                               <  2:1031402/140‑1 (MQ=255)                                                                                                        gggCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAg                               <  2:2017552/140‑1 (MQ=255)                                                                                                        gggCAGGCAATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAg                               >  1:1735916/1‑140 (MQ=255)                                                                                                         ggcaggcaATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGt                              >  2:1975968/1‑140 (MQ=255)                                                                                                           caggcaATCAGCAGCAACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGc                            <  2:1617809/140‑1 (MQ=255)                                                                                                               caATCAGCAGCAACGTGATCATCGGTTAAGAACCGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGCCACt                        <  1:326340/140‑1 (MQ=255)                                                                                                                      cagcaACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGCCACtacgctta                 <  2:606753/140‑1 (MQ=255)                                                                                                                      cagcaACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGCCACtacgctta                 <  2:1561510/140‑1 (MQ=255)                                                                                                                      cagcaACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGCCACtacgctta                 <  2:44551/140‑1 (MQ=255)                                                                                                                      cagcaACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGCCACtacgctta                 <  1:103271/140‑1 (MQ=255)                                                                                                                       agcaACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGACAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGCCACtacgcttac                >  2:355325/1‑140 (MQ=255)                                                                                                                        gcaACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGCCACtacgcttacg               <  2:435039/140‑1 (MQ=255)                                                                                                                        gcaACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGCCACtacgcttacg               >  2:335342/1‑140 (MQ=255)                                                                                                                        gcaACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGCCACtacgcttacg               <  1:335342/140‑1 (MQ=255)                                                                                                                        gcaACGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGCCACtacgcttacg               <  1:608770/140‑1 (MQ=255)                                                                                                                            cGTGATCATCGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGCCACTACGCTTACGCtca           >  1:199648/1‑140 (MQ=255)                                                                                                                                atcatcGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGCCACTACGCTTACGCTCATCAg       >  2:1687332/1‑140 (MQ=255)                                                                                                                                 tcatcGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGCCACTACGCTTACGCTCATCAgg      >  1:1786701/1‑140 (MQ=255)                                                                                                                                   atcGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGCCACTACGCTTACGCTCATCAGGAg    <  1:1975968/140‑1 (MQ=255)                                                                                                                                     cGGTTAAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGCCACTACGCTTACGCTCATCAGGAgcg  >  1:184512/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       CATTGATTTTTCGTGGTCGTAACCGCACACCCAGGCCGCACCGCGGGAACGCGATGGCAAACGATCGCCTTTGCAAATCGCCATAATGATGAATGGCAGCAGCGGGCAGGCAATCAGCAGCAACGTGATCATCGGTTGAGAAACGGTGGTGTTAGCAGGCTCCAGCGGCAGAGGTACAGCAGCAGAGAGCATCGGCAGTAGCCACGGCGCAGCAACACCGCCAATTACGCAGCAAATCGCCAGTGCCACTACGCTTACGCTCATCAGGAGCG  >  NC_000913/2847607‑2847878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑