Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,598,064	C→A	G460W (GGG→TGG)	opgB ←	phosphoglycerol transferase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,598,064	0	C	A	100.0%	347.5 / NA	97	G460W (GGG→TGG)	opgB	phosphoglycerol transferase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (53/44);  total (53/44)

GAAGGCAATCATGTTTTTCTGCTGGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCCGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAAC  >  NC_000913/4597927‑4598200                                                                                                                                          |                                                                                                                                         gaAGGCAATCATGTTTTTCTGCTGGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAgg                                                                                                                                        >  2:650885/1‑140 (MQ=255)  aaGGCAATCATGTTTTTCTGCTGGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGa                                                                                                                                       >  1:670501/1‑140 (MQ=255)  aaGGCAATCATGTTTTTCTGCTGGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGa                                                                                                                                       <  2:819446/140‑1 (MQ=255)  aaGGCAATCATGTTTTTCTGCTGGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGa                                                                                                                                       >  1:767834/1‑140 (MQ=255)     gCAATCATGTTTTTCTGCTGGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATaa                                                                                                                                    >  2:1060792/1‑140 (MQ=255)     gCAATCATGTTTTTCTGCTGGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATaa                                                                                                                                    >  1:1312149/1‑140 (MQ=255)       aaTCATGTTTTTCTGCTGGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAAc                                                                                                                                  >  1:911069/1‑140 (MQ=255)         tCATGTTTTTCTGCTGGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTg                                                                                                                                >  1:33068/1‑140 (MQ=255)                 ttCTGCTGGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGAcc                                                                                                                        <  1:1691853/140‑1 (MQ=255)                 ttCTGCTGGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGAcc                                                                                                                        <  1:943402/140‑1 (MQ=255)                 ttCTGCTGGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGAcc                                                                                                                        <  2:2005574/140‑1 (MQ=255)                 ttCTGCTGGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGAcc                                                                                                                        <  1:1060792/140‑1 (MQ=255)                     gctgGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGt                                                                                                                    >  2:1637026/1‑140 (MQ=255)                      ctgGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCaa                                                                                                                      <  1:1064204/137‑1 (MQ=255)                      ctgGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCaa                                                                                                                      >  2:1064204/1‑137 (MQ=255)                       tgGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTcc                                                                                                                  >  1:1628463/1‑140 (MQ=255)                        ggTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCATGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCg                                                                                                                 >  1:1738687/1‑140 (MQ=255)                          tCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCgag                                                                                                               >  1:218691/1‑140 (MQ=255)                          tCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCgag                                                                                                               <  2:1740968/140‑1 (MQ=255)                           cGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCATGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCgaga                                                                                                              <  2:1738687/140‑1 (MQ=255)                           cGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCgaga                                                                                                              <  2:627818/140‑1 (MQ=255)                           cGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCgaga                                                                                                              <  2:1464985/140‑1 (MQ=255)                              tGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGGTAg                                                                                                           >  1:1588682/1‑140 (MQ=255)                                  gAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTAt                                                                                                       <  2:1621261/140‑1 (MQ=255)                                        tttCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGc                                                                                                    <  2:1569923/137‑1 (MQ=255)                                        tttCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGc                                                                                                    >  1:1569923/1‑137 (MQ=255)                                        tttCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCAc                                                                                                 >  2:1313953/1‑140 (MQ=255)                                        tttCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCAc                                                                                                 <  2:1222239/140‑1 (MQ=255)                                        tttCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCAc                                                                                                 <  2:1628463/140‑1 (MQ=255)                                        tttCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCAc                                                                                                 >  2:350986/1‑140 (MQ=255)                                        tttCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCAc                                                                                                 >  1:126849/1‑140 (MQ=255)                                        tttCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCAc                                                                                                 <  2:670501/140‑1 (MQ=255)                                        tttCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCAc                                                                                                 >  2:981239/1‑140 (MQ=255)                                          tCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCg                                                                                               >  1:1220816/1‑140 (MQ=255)                                             tctcTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCgaga                                                                                            <  1:1038059/140‑1 (MQ=255)                                             tctcTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCgaga                                                                                            <  1:76682/140‑1 (MQ=255)                                             tctcTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCgaga                                                                                            <  1:1637026/140‑1 (MQ=255)                                             tctcTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCgaga                                                                                            <  1:1582633/140‑1 (MQ=255)                                             tctcTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCgaga                                                                                            <  1:1436738/140‑1 (MQ=255)                                             tctcTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCgaga                                                                                            <  1:302503/140‑1 (MQ=255)                                                ctTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATg                                                                                         >  1:195428/1‑140 (MQ=255)                                                  ttAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTc                                                                                       >  1:346082/1‑140 (MQ=255)                                                      ggAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGc                                                                                   >  1:1378065/1‑140 (MQ=255)                                                           ttCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGt                                                                              >  2:1980769/1‑140 (MQ=255)                                                           ttCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGt                                                                              >  1:376163/1‑140 (MQ=255)                                                             ccACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTcg                                                                            <  1:1313953/140‑1 (MQ=255)                                                             ccACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTcg                                                                            <  1:981239/140‑1 (MQ=255)                                                             ccACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTcg                                                                            <  2:1571810/140‑1 (MQ=255)                                                               acaGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTcgcg                                                                          <  2:346082/140‑1 (MQ=255)                                                               acaGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTcgcg                                                                          <  1:766707/140‑1 (MQ=255)                                                               acaGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTcgcg                                                                          <  2:610001/140‑1 (MQ=255)                                                               acaGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTcgcg                                                                          >  1:1049316/1‑140 (MQ=255)                                                               acaGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTcgcg                                                                          <  1:1980769/140‑1 (MQ=255)                                                                      gatgatATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTAt                                                                   >  2:197388/1‑140 (MQ=255)                                                                      gatgatATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTAt                                                                   <  2:1598007/140‑1 (MQ=255)                                                                      gatgatATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTAt                                                                   >  2:899008/1‑140 (MQ=255)                                                                       atgatATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATc                                                                  <  1:387746/140‑1 (MQ=255)                                                                         gatATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCa                                                                <  2:376163/140‑1 (MQ=255)                                                                         gatATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCa                                                                <  2:1049316/140‑1 (MQ=255)                                                                           tatCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATc                                                              <  1:197388/140‑1 (MQ=255)                                                                                 gCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCa                                                                <  1:769419/132‑1 (MQ=255)                                                                                 gCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCa                                                                >  2:769419/1‑132 (MQ=255)                                                                                 gCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTa                                                        >  2:48300/1‑140 (MQ=255)                                                                                 gCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTa                                                        <  1:350986/140‑1 (MQ=255)                                                                                    tCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGc                                                     >  1:1272066/1‑140 (MQ=255)                                                                                     ccACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCt                                                    >  1:441902/1‑140 (MQ=255)                                                                                     ccACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCt                                                    >  1:1889091/1‑140 (MQ=255)                                                                                     ccACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCt                                                    >  1:1583279/1‑140 (MQ=255)                                                                                      cACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCtt                                                   >  1:400606/1‑140 (MQ=255)                                                                                      cACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCtt                                                   >  2:944459/1‑140 (MQ=255)                                                                                              tGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCa                                           <  1:944459/140‑1 (MQ=255)                                                                                              tGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCa                                           <  2:33068/140‑1 (MQ=255)                                                                                                   tctcTTTGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGtc                                      >  2:1552473/1‑140 (MQ=255)                                                                                                       tttGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCtt                                  >  2:452790/1‑140 (MQ=255)                                                                                                       tttGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCtt                                  >  2:113652/1‑140 (MQ=255)                                                                                                       tttGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCtt                                  >  1:285185/1‑140 (MQ=255)                                                                                                         tGATATTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTtgc                                >  2:1049318/1‑140 (MQ=255)                                                                                                           atatTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTtgctg                              <  1:48300/140‑1 (MQ=255)                                                                                                            tatTGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTtgctgc                             >  1:1012875/1‑140 (MQ=255)                                                                                                               tGAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGc                          >  1:1119793/1‑140 (MQ=255)                                                                                                                gAGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCt                         >  2:124035/1‑140 (MQ=255)                                                                                                                 aGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCtt                        <  1:899008/140‑1 (MQ=255)                                                                                                                 aGGAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCtt                        >  2:1463823/1‑140 (MQ=255)                                                                                                                   gAAGATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGt                      >  2:1615922/1‑140 (MQ=255)                                                                                                                     aGATTTGCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCg                    >  1:1737392/1‑140 (MQ=255)                                                                                                                      gATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGc                   >  1:470192/1‑140 (MQ=255)                                                                                                                      gATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGc                   >  1:737740/1‑140 (MQ=255)                                                                                                                      gATTTCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGc                   >  2:891389/1‑140 (MQ=255)                                                                                                                         ttCCGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCAc                >  2:1666949/1‑140 (MQ=255)                                                                                                                           ccGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGa              >  2:1156654/1‑140 (MQ=255)                                                                                                                            cGACATCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGaa             <  1:1552473/140‑1 (MQ=255)                                                                                                                                aTCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGAc         <  1:452790/140‑1 (MQ=255)                                                                                                                                 tCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACa        <  2:1583279/140‑1 (MQ=255)                                                                                                                                 tCGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACa        >  1:168248/1‑140 (MQ=255)                                                                                                                                  cGACTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACaa       >  1:1547230/1‑140 (MQ=255)                                                                                                                                     cTGCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACaaaaa    <  2:737740/140‑1 (MQ=255)                                                                                                                                       gCCAGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAAc  <  1:124035/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GAAGGCAATCATGTTTTTCTGCTGGTCGATGGTGAACTCTTTCATCTCTTTAGGGAATTTCCACAGGCGGATGATATCTGGCTTCCACGCCAATGTTTTCTCTTTGATATTGAGGAAGATTTCCGACATCGACTGCCCGGATAAACTGCTACGACCAAGTCCGAGATAGTTATCGCCACCGAGAATGTCCAGCACCGTCGCGCCGTTATCCATCGTGTTACGCTTCACTGCCAGCGTCTCTTGCTGCGGCTTGTCGCCACGAATGACAAAAAAC  >  NC_000913/4597927‑4598200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑