Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,332,361	G→T	M438I (ATG→ATT)	topA →	DNA topoisomerase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,332,361	0	G	T	100.0%	298.3 / NA	84	M438I (ATG→ATT)	topA	DNA topoisomerase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (31/53);  total (31/53)

TTAATCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGG  >  NC_000913/1332224‑1332494                                                                                                                                          |                                                                                                                                      ttAATCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTcc                                                                                                                                     >  2:921830/1‑140 (MQ=255) ttAATCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTcc                                                                                                                                     >  1:1804034/1‑140 (MQ=255)   aaTCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCtg                                                                                                                                   >  1:1310077/1‑140 (MQ=255)     tCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCtgcg                                                                                                                                 <  1:1753742/140‑1 (MQ=255)     tCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCtgcg                                                                                                                                 <  2:505134/140‑1 (MQ=255)     tCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCtgcg                                                                                                                                 >  1:317458/1‑140 (MQ=255)     tCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCtgcg                                                                                                                                 <  2:1010781/140‑1 (MQ=255)     tCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCtgcg                                                                                                                                 <  2:717879/140‑1 (MQ=255)     tCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCtgcg                                                                                                                                 <  2:1391148/140‑1 (MQ=255)             cAGTTCGTTGCCTGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTaa                                                                                                                         <  2:1421544/140‑1 (MQ=255)             cAGTTCGTTGCCTGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTaa                                                                                                                         <  1:1582105/140‑1 (MQ=255)                       cctgccAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGaa                                                                                                               >  2:277936/1‑140 (MQ=255)                        ctgccAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAg                                                                                                              >  1:267583/1‑140 (MQ=255)                        ctgccAGATGACCCCAGCG‑AATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGa                                                                                                             >  1:530522/1‑140 (MQ=255)                          gccAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTTGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGAt                                                                                                            <  2:267583/140‑1 (MQ=255)                          gccAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGAt                                                                                                            >  1:779118/1‑140 (MQ=255)                          gccAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGAt                                                                                                            >  2:414414/1‑140 (MQ=255)                             aGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGc                                                                                                         <  1:306932/140‑1 (MQ=255)                             aGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGc                                                                                                         <  1:1987057/140‑1 (MQ=255)                               aTGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGc                                                                                                         <  2:685177/138‑1 (MQ=255)                               aTGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGc                                                                                                         >  1:685177/1‑138 (MQ=255)                               aTGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCAt                                                                                                       <  1:288290/140‑1 (MQ=255)                                   ccccAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTa                                                                                                   >  2:1982767/1‑140 (MQ=255)                                    cccAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTAc                                                                                                  <  2:1310077/140‑1 (MQ=255)                                     ccAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTAcc                                                                                                 <  1:2028754/140‑1 (MQ=255)                                     ccAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTAcc                                                                                                 <  1:1143948/140‑1 (MQ=255)                                     ccAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTAcc                                                                                                 <  1:187235/140‑1 (MQ=255)                                      cAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACca                                                                                                >  1:1706769/1‑140 (MQ=255)                                      cAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGAC‑AAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACcag                                                                                               <  1:508356/140‑1 (MQ=255)                                       aGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACcag                                                                                               <  1:277936/140‑1 (MQ=255)                                         gcgaaTATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACcagca                                                                                             <  2:530522/137‑1 (MQ=255)                                         cGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACcagca                                                                                             >  2:1622699/1‑140 (MQ=255)                                         cGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACcagca                                                                                             <  2:196026/140‑1 (MQ=255)                                         cGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACcagca                                                                                             <  2:1255592/140‑1 (MQ=255)                                         cGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACcagca                                                                                             <  1:979238/140‑1 (MQ=255)                                                 gACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTtaataa                                                                                     <  2:1542885/140‑1 (MQ=255)                                                    tCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAgg                                                                                  <  1:1037329/140‑1 (MQ=255)                                                      cACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCg                                                                                <  2:601886/140‑1 (MQ=255)                                                      cACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCg                                                                                <  1:414414/140‑1 (MQ=255)                                                       acgacgCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGa                                                                               <  2:1699830/140‑1 (MQ=255)                                                        cgacgCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGAt                                                                              <  1:921830/140‑1 (MQ=255)                                                         gacgCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATg                                                                             <  1:187236/140‑1 (MQ=255)                                                              tGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTg                                                                        >  2:1938166/1‑140 (MQ=255)                                                              tGACCGTTGGTGCGGGCGATTTCCGCCTAAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTg                                                                        >  1:487531/1‑140 (MQ=255)                                                                aCCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGAc                                                                      >  2:591817/1‑140 (MQ=255)                                                                     tGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCg                                                                 >  2:1646796/1‑140 (MQ=255)                                                                     tGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCg                                                                 >  2:1392308/1‑140 (MQ=255)                                                                     tGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCg                                                                 >  1:1315367/1‑140 (MQ=255)                                                                     tGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCg                                                                 >  2:1180601/1‑140 (MQ=255)                                                                      ggTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGt                                                                >  2:984570/1‑140 (MQ=255)                                                                      ggTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGt                                                                <  1:1730940/140‑1 (MQ=255)                                                                       gTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGtt                                                               <  2:1360158/140‑1 (MQ=255)                                                                       gTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGtt                                                               <  2:317458/140‑1 (MQ=255)                                                                       gTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGtt                                                               <  2:1869974/140‑1 (MQ=255)                                                                       gTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGtt                                                               <  2:1706769/140‑1 (MQ=255)                                                                       gTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGtt                                                               <  1:591817/140‑1 (MQ=255)                                                                          cGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGaa                                                            >  1:1723131/1‑140 (MQ=255)                                                                          cGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGaa                                                            >  1:417885/1‑140 (MQ=255)                                                                            ggCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACt                                                          >  2:864372/1‑140 (MQ=255)                                                                              cGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACt                                                          <  1:1010998/138‑1 (MQ=255)                                                                              cGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACt                                                          >  2:1010998/1‑138 (MQ=255)                                                                                 tttCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTacac                                                     <  1:1938166/140‑1 (MQ=255)                                                                                 tttCCGCCTAAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTacac                                                     <  2:487531/140‑1 (MQ=255)                                                                                      gCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACAccagcc                                                <  1:1392308/140‑1 (MQ=255)                                                                                       ccTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGaa                                                            <  2:1267167/127‑1 (MQ=255)                                                                                       ccTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGaa                                                            >  1:1267167/1‑127 (MQ=255)                                                                                           aaaGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCa                                           <  2:1804034/140‑1 (MQ=255)                                                                                                   cgGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCa                                   <  2:779118/140‑1 (MQ=255)                                                                                                             ttGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGc                         <  2:1315367/140‑1 (MQ=255)                                                                                                              tGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGcc                        <  1:1982767/140‑1 (MQ=255)                                                                                                              tGCGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGcc                        <  2:1411011/140‑1 (MQ=255)                                                                                                                cGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCg                      >  1:1307814/1‑140 (MQ=255)                                                                                                                cGTTTTGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCg                      >  2:1643650/1‑140 (MQ=255)                                                                                                                   tttGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGttt                   <  1:864372/140‑1 (MQ=255)                                                                                                                    ttGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTc                  <  1:1643650/140‑1 (MQ=255)                                                                                                                    ttGATGGCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTc                  >  1:909173/1‑140 (MQ=255)                                                                                                                         ggCTGGACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGa             <  1:984570/140‑1 (MQ=255)                                                                                                                              gACAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCAt        >  1:1605319/1‑140 (MQ=255)                                                                                                                               aCAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATc       <  2:417885/140‑1 (MQ=255)                                                                                                                               aCAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATc       <  1:1646796/140‑1 (MQ=255)                                                                                                                               aCAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATc       <  1:1180601/140‑1 (MQ=255)                                                                                                                                cAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCg      <  1:1048943/140‑1 (MQ=255)                                                                                                                                cAAAAGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCg      <  1:1021209/140‑1 (MQ=255)                                                                                                                                    aGTGATTCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTgg  >  1:1581700/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      TTAATCTGGCGTCAGTTCGTTGCCTGCCAGATGACCCCAGCGAAATATGACTCCACGACGCTGACCGTTGGTGCGGGCGATTTCCGCCTGAAAGCACGCGGTCGTATTTTGCGTTTTGATGGCTGGACAAAAGTGATGCCTGCGTTGCGTAAAGGCGATGAAGATCGCATCTTACCAGCAGTTAATAAAGGCGATGCTCTGACGCTCGTTGAACTTACACCAGCCCAGCACTTTACCAAGCCGCCAGCCCGTTTCAGTGAAGCATCGCTGG  >  NC_000913/1332224‑1332494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑