Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,984,362	C→A	intergenic (‑238/+49)	kduI ← / ← yqeF	5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase/putative acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,984,362	0	C	A	100.0%	144.3 / NA	41	intergenic (‑238/+49)	kduI/yqeF	5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase/putative acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/22);  total (19/22)

TTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATT  >  NC_000913/2984225‑2984488                                                                                                                                          |                                                                                                                               ttCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTaaa                                                                                                                              <  1:518242/140‑1 (MQ=255) ttCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTaaa                                                                                                                              >  2:518242/1‑140 (MQ=255) ttCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTaaa                                                                                                                              <  1:1382711/140‑1 (MQ=255) ttCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTaaa                                                                                                                              >  2:365952/1‑140 (MQ=255) ttCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTaaa                                                                                                                              >  1:1520748/1‑140 (MQ=255)          aaaTAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAAt                                                                                                                     <  2:1320390/140‑1 (MQ=255)               aaaCAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACgg                                                                                                                <  2:1269479/140‑1 (MQ=255)                 aCAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCa                                                                                                              <  1:507215/140‑1 (MQ=255)                     tGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGata                                                                                                          >  1:346296/1‑140 (MQ=255)                         tCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTa                                                                                                      <  1:365952/140‑1 (MQ=255)                               ttaagttaagGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTa                                                                                                >  2:600152/1‑140 (MQ=255)                                  agttaagGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAagg                                                                                             <  2:1520748/140‑1 (MQ=255)                                     taagGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAaggagg                                                                                          <  1:571353/140‑1 (MQ=255)                                      aagGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAggagga                                                                                         >  1:1444115/1‑140 (MQ=255)                                       agGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGaa                                                                                        <  2:308827/140‑1 (MQ=255)                                          aTTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATg                                                                                     <  1:988816/140‑1 (MQ=255)                                              aaaaaaGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCaga                                                                                 >  1:1295711/1‑140 (MQ=255)                                              aaaaaaGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCaga                                                                                 >  2:1001704/1‑140 (MQ=255)                                               aaaaaGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCagag                                                                                <  2:1016368/140‑1 (MQ=255)                                                aaaaGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGc                                                                               <  2:1018566/140‑1 (MQ=255)                                                   aGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTAt                                                                            >  2:1159917/1‑140 (MQ=255)                                                   aGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTAt                                                                            <  2:508439/140‑1 (MQ=255)                                                        aGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTc                                                                       <  2:964622/140‑1 (MQ=255)                                                        aGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTc                                                                       <  2:968981/140‑1 (MQ=255)                                                        aGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTc                                                                       <  1:1341577/140‑1 (MQ=255)                                                             cGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGtt                                                                  <  2:1083277/140‑1 (MQ=255)                                                               aTCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCa                                                                >  2:1083831/1‑140 (MQ=255)                                                               aTCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCa                                                                >  1:359490/1‑140 (MQ=255)                                                               aTCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCa                                                                >  2:448946/1‑140 (MQ=255)                                                               aTCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCa                                                                <  1:1400668/140‑1 (MQ=255)                                                                    gAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGt                                                           <  1:1001704/140‑1 (MQ=255)                                                                    gAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGt                                                           <  1:448946/140‑1 (MQ=255)                                                                    gAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGt                                                           <  2:1494013/140‑1 (MQ=255)                                                                      atatGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCa                                                         <  1:1334907/140‑1 (MQ=255)                                                                      atatGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCa                                                         >  2:21264/1‑140 (MQ=255)                                                                      atatGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCa                                                         >  1:1441829/1‑140 (MQ=255)                                                                          gTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGc                                                     >  2:428817/1‑140 (MQ=255)                                                                           tCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGcc                                                    <  1:280576/140‑1 (MQ=255)                                                                           tCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGcc                                                    <  1:1149819/140‑1 (MQ=255)                                                                              tGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCcaca                                                 >  1:336228/1‑140 (MQ=255)                                                                                  aaTGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCt                                             >  2:1106003/1‑140 (MQ=255)                                                                                     gnAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCNCGTTCAATGGTCAATGCCANACCCTggn                                          >  1:354693/1‑139 (MQ=255)                                                                                                ggAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATa                               >  1:814622/1‑140 (MQ=255)                                                                                                     ccAGAAAATGTATGTCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAg                          >  1:617725/1‑140 (MQ=255)                                                                                                      aaaaaaaTTTATGTCGACTGACCAGCCATCATTATAAAACAAATTAAGGACGGCAAATTTTTATCGGAAAGGAGAAAATGCAGAGCTATTCGCCCGTTTAAATGGTCAACGCCCCCCCCTGCCCCCCGCCGATACAAAGc                         <  2:617725/137‑1 (MQ=255)                                                                                                      aaGAAAATGTATGTCGACTTGCCAGCCATTATTATAAAACAGATTAATGACGGCAGATATTTATGGGTAAGGAGGAAAGGCAGAGTTTTTCGTCACGTTCAATGGTCAATGCCACCCCCGGGCCCCCGCCGATACAAAGc                         <  2:47008/139‑1 (MQ=255)                                                                                                              gtatgtCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAg                 >  1:404294/1‑140 (MQ=255)                                                                                                                 tgtCGACTTGCCAGCCATCATTATTAAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTcc              >  1:1143704/1‑140 (MQ=255)                                                                                                                             ccccaTAATTATTAAAAAGATTAAGGAGGGAAGATTTTTATCGGAAGGGAGAAAATGCAGAGTTTTTCGCCACTTTCAAGGGTCAATCCCCCCCCCTGCCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAtt  <  2:1455831/138‑1 (MQ=255)                                                                                                                                          |                                                                                                                               TTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATT  >  NC_000913/2984225‑2984488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑