Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,622,646	G→T	G1152V (GGC→GTC)	rhsB →	rhs element protein RhsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,622,646	0	G	T	100.0%	232.8 / NA	84	G1152V (GGC→GTC)	rhsB	rhs element protein RhsB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (51/33);  total (51/33)

GGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGT  >  NC_000913/3622520‑3622777                                                                                                                               |                                                                                                                                    gggTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGgcg                                                                                                                        >  2:1300232/1‑140 (MQ=11) gggTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGgcg                                                                                                                        <  2:732250/140‑1 (MQ=11) gggTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGgcg                                                                                                                        >  1:1359467/1‑140 (MQ=11) gggTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGgcg                                                                                                                        <  1:157114/140‑1 (MQ=11)    tgagtgagGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCtc                                                                                                                     >  1:1333257/1‑140 (MQ=17)      agtgagGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCtcat                                                                                                                   >  1:914192/1‑140 (MQ=17)         gagGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAGAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAg                                                                                                                <  2:1285361/140‑1 (MQ=12)          agGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCtcat                                                                                                                   <  2:133257/136‑1 (MQ=17)          agGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCtcat                                                                                                                   >  1:133257/1‑136 (MQ=17)          agGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGC‑AAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCa                                                                                                              <  2:1336097/140‑1 (MQ=12)           ggAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACCCGCCGGCGCGAAAAATCCACCTGTACCCCTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCa                                                                                                              <  2:1386020/140‑1 (MQ=12)           ggAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCa                                                                                                              <  1:401016/140‑1 (MQ=17)           ggAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCa                                                                                                              <  2:783668/140‑1 (MQ=17)             aaaGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACg                                                                                                            >  2:1360082/1‑140 (MQ=17)              aaGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACgg                                                                                                           >  1:103465/1‑140 (MQ=17)                 ccgccgCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAg                                                                                                        >  2:1410762/1‑140 (MQ=17)                    ccgcTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAgggg                                                                                                     >  1:732855/1‑140 (MQ=17)                       ctggctggCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCaa                                                                                                  >  1:231047/1‑140 (MQ=17)                            tggcATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCg                                                                                             <  2:1178684/140‑1 (MQ=17)                                  cGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTgc                                                                                       <  2:383968/140‑1 (MQ=17)                                  cGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTgc                                                                                       <  1:1450971/140‑1 (MQ=17)                                   gTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTgcg                                                                                      <  1:1410762/140‑1 (MQ=17)                                   gTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTgcg                                                                                      <  2:103465/140‑1 (MQ=17)                                   gTGCGGCATGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTgcg                                                                                      <  2:1309060/140‑1 (MQ=17)                                    tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTgcgc                                                                                     >  1:657289/1‑140 (MQ=18)                                    tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTgcgc                                                                                     >  1:1046959/1‑140 (MQ=17)                                    tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTgcgc                                                                                     >  1:1170814/1‑140 (MQ=18)                                    tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTgcgc                                                                                     >  1:702737/1‑140 (MQ=18)                                    tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTgcgc                                                                                     >  1:623760/1‑140 (MQ=18)                                    tGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTgcgc                                                                                     >  1:286874/1‑140 (MQ=18)                                         ccTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAat                                                                                >  2:87384/1‑140 (MQ=18)                                             aCCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATatga                                                                            >  2:521997/1‑140 (MQ=17)                                             aCCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATatga                                                                            <  2:1170814/140‑1 (MQ=17)                                                   gAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATg                                                                      <  1:87384/140‑1 (MQ=17)                                                         aTGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCaa                                                                >  1:637552/1‑140 (MQ=18)                                                         aTGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCaa                                                                >  2:1016607/1‑140 (MQ=18)                                                            cAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACct                                                             >  1:610668/1‑140 (MQ=11)                                                                    gATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCtgaatg                                                     >  1:1324313/1‑140 (MQ=17)                                                                     aTGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCtgaa                                                       <  2:182960/137‑1 (MQ=11)                                                                     aTGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCtgaa                                                       >  1:182960/1‑137 (MQ=11)                                                                      ggggccaggTGGACAAGGCGGCGCGAAAAATCGCCGTCTACCACGGCGACCATCTCGTCCTAGCGGTGGCGCGCGTTGTCACGGAAGGGGCAGCAGGGTGGTGCGCAGTATTTGATGAATGGGGCAACCTGAtgaaggaa                                                   <  2:138906/133‑1 (MQ=2)                                                                              gtgtACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAAccc                                           >  1:1304868/1‑140 (MQ=21)                                                                                 tACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCa                                        <  1:744071/140‑1 (MQ=37)                                                                                   cacGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAaga                                                 >  2:267571/1‑129 (MQ=25)                                                                                   cacGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAaga                                                 <  1:267571/129‑1 (MQ=25)                                                                                    acGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCa                                     >  1:1177109/1‑140 (MQ=21)                                                                                    acGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCa                                     <  1:1307954/140‑1 (MQ=37)                                                                                        cGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTg                                 >  2:820491/1‑140 (MQ=37)                                                                                        cGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAA‑GGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCCACCTTCTGAATTGAGAGAACCCGCATccacctc                                >  2:725011/1‑134 (MQ=11)                                                                                         ggCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgc                                <  2:1046959/140‑1 (MQ=37)                                                                                         ggCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgc                                >  2:1413789/1‑140 (MQ=37)                                                                                         ggCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgc                                >  1:979739/1‑140 (MQ=37)                                                                                          gcgcGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgca                               <  1:1360082/140‑1 (MQ=38)                                                                                          gcgcGAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgca                               <  2:1177109/140‑1 (MQ=37)                                                                                           cgcgAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgcag                              >  1:1499824/1‑140 (MQ=37)                                                                                           cgcgAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgcag                              >  2:206790/1‑140 (MQ=37)                                                                                           cgcgAAAAATCCACCTGTACCACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACAGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTgcag                              >  2:270796/1‑140 (MQ=255)                                                                                                              ccACTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCgg           >  2:1459007/1‑140 (MQ=255)                                                                                                                aCTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCgggg         <  2:743979/140‑1 (MQ=255)                                                                                                                aCTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCgggg         <  2:637552/140‑1 (MQ=255)                                                                                                                aCTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCgggg         <  1:1016607/140‑1 (MQ=255)                                                                                                                 cTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgc        >  2:336336/1‑140 (MQ=255)                                                                                                                 cTGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgc        >  2:1488885/1‑140 (MQ=255)                                                                                                                  tGCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgca       >  2:1095948/1‑140 (MQ=255)                                                                                                                   gCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcag      >  2:1191518/1‑140 (MQ=255)                                                                                                                   gCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcag      >  1:88635/1‑140 (MQ=255)                                                                                                                   gCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcag      <  1:871991/140‑1 (MQ=255)                                                                                                                   gCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcag      >  2:605374/1‑140 (MQ=255)                                                                                                                   gCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcag      <  1:1459007/140‑1 (MQ=255)                                                                                                                   gCGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcag      <  2:702737/140‑1 (MQ=255)                                                                                                                    cGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCgggg         >  2:224384/1‑136 (MQ=255)                                                                                                                    cGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCgggg         <  1:224384/136‑1 (MQ=255)                                                                                                                    cGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcagc     >  2:270179/1‑140 (MQ=255)                                                                                                                    cGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcagc     >  1:805117/1‑140 (MQ=255)                                                                                                                    cGACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATAGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTACCCGCTTCCGGGGgcagc     >  1:443463/1‑140 (MQ=14)                                                                                                                     gACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcagca    <  2:1359467/140‑1 (MQ=255)                                                                                                                     gACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcagca    >  2:898860/1‑140 (MQ=255)                                                                                                                     gACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcagca    <  1:1300232/140‑1 (MQ=255)                                                                                                                     gACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcagca    <  2:1225103/140‑1 (MQ=255)                                                                                                                     gACCATCGCGTCCTGCCGCTGGCGCTCATCAGCACAGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGgcagca    <  1:270796/140‑1 (MQ=255)                                                                                                                      aCCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGcagcag   >  1:829441/1‑140 (MQ=255)                                                                                                                      aCCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGcagcag   >  2:794595/1‑140 (MQ=255)                                                                                                                      aCCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGcagcag   >  2:608756/1‑140 (MQ=255)                                                                                                                      aCCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGcagcag   >  2:85814/1‑140 (MQ=255)                                                                                                                      aCCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGcagcag   >  2:984646/1‑140 (MQ=255)                                                                                                                      aCCATCGCGTCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAA‑GGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGt  >  2:315725/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                    GGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCGCTCATCAGCACGGAAGGGGCAACAGCGTGGTGCGCAGAATATGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGCTGCAGCAGCTTATCCGGCTGCCGGGGCAGCAGT  >  NC_000913/3622520‑3622777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑