Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,878,666	G→T	P485Q (CCG→CAG)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,878,666	0	G	T	100.0%	258.7 / NA	74	P485Q (CCG→CAG)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (37/37);  total (37/37)

GAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAAT  >  NC_000913/3878529‑3878793                                                                                                                                          |                                                                                                                                gAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCtgg                                                                                                                               >  1:1166882/1‑140 (MQ=255)  aGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGt                                                                                                                              <  2:1188789/140‑1 (MQ=255)      aTGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTa                                                                                                                          <  2:1036698/140‑1 (MQ=255)      aTGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTa                                                                                                                          <  2:1313410/140‑1 (MQ=255)         tAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTc                                                                                                                       >  2:399607/1‑140 (MQ=255)               tGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACg                                                                                                                 <  1:700487/140‑1 (MQ=255)               tGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACg                                                                                                                 <  1:1151078/140‑1 (MQ=255)                 aCCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGAc                                                                                                               >  1:1003685/1‑140 (MQ=255)                 aCCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGAc                                                                                                               >  2:947242/1‑140 (MQ=255)                 aCCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGAc                                                                                                               >  1:1301127/1‑140 (MQ=255)                 aCCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGAc                                                                                                               >  1:1482876/1‑140 (MQ=255)                 aCCGCGTTCAACGATTTCCGACATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGACGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGAc                                                                                                               >  1:1299445/1‑140 (MQ=255)                  ccGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGAcc                                                                                                              <  1:1514997/140‑1 (MQ=255)                   cgcgTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCg                                                                                                             <  1:105515/140‑1 (MQ=255)                      gTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATa                                                                                                          >  2:534272/1‑140 (MQ=255)                       ttCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATAc                                                                                                         >  2:525953/1‑140 (MQ=255)                       ttCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATAc                                                                                                         >  1:969120/1‑140 (MQ=255)                              aTTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGcc                                                                                                  <  1:1331712/140‑1 (MQ=255)                                  ccGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAgc                                                                                              <  1:394538/140‑1 (MQ=255)                                   cGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAgcg                                                                                             <  1:525953/140‑1 (MQ=255)                                    ggCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAgcgc                                                                                            <  2:1348624/140‑1 (MQ=255)                                    ggCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAgcgc                                                                                            <  1:61036/140‑1 (MQ=255)                                           gACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATa                                                                                     <  2:422876/140‑1 (MQ=255)                                              gATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGc                                                                                  <  2:1166882/140‑1 (MQ=255)                                               aTAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCg                                                                                 <  1:399607/140‑1 (MQ=255)                                               aTAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCg                                                                                 <  1:724641/140‑1 (MQ=255)                                                   aagaagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGc                                                                             >  1:301758/1‑140 (MQ=255)                                                   aagaagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGc                                                                             >  2:934896/1‑140 (MQ=255)                                                   aagaagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGc                                                                             <  1:534272/140‑1 (MQ=255)                                                    agaagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTTCTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGcc                                                                            <  1:354757/140‑1 (MQ=255)                                                    agaagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGcc                                                                            <  1:912795/140‑1 (MQ=255)                                                      aagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCAc                                                                          <  1:692441/140‑1 (MQ=255)                                                      aagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCAc                                                                          <  1:1518638/140‑1 (MQ=255)                                                      aagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCAc                                                                          <  2:693907/140‑1 (MQ=255)                                                      aagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCAc                                                                          <  1:475742/140‑1 (MQ=255)                                                        ggTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACtt                                                                        >  2:268372/1‑140 (MQ=255)                                                             aCAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTga                                                                   >  2:1179438/1‑140 (MQ=255)                                                                 cagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaag                                                               >  2:97999/1‑140 (MQ=255)                                                                  agcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaaga                                                              >  2:284769/1‑140 (MQ=255)                                                                  agcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaaga                                                              <  1:97999/140‑1 (MQ=255)                                                                   gcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTg                                                                    >  2:13357/1‑133 (MQ=255)                                                                   gcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTg                                                                    <  1:13357/133‑1 (MQ=255)                                                                    cGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaag                                                               >  1:665090/1‑137 (MQ=255)                                                                    cGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaag                                                               <  2:665090/137‑1 (MQ=255)                                                                    cGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagaga                                                            >  1:475063/1‑140 (MQ=255)                                                                     gTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagagag                                                           <  2:301758/140‑1 (MQ=255)                                                                     gTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagagag                                                           <  2:909044/140‑1 (MQ=255)                                                                         gAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATc                                                       <  1:947242/140‑1 (MQ=255)                                                                            tgtgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTa                                                    <  2:449046/140‑1 (MQ=255)                                                                              tgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaaga                                                              >  2:729027/1‑128 (MQ=255)                                                                              tgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaaga                                                              <  1:729027/128‑1 (MQ=255)                                                                              tgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATc                                                  >  2:984835/1‑140 (MQ=255)                                                                              tgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATc                                                  >  2:1272549/1‑140 (MQ=255)                                                                                cGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGa                                                >  2:390442/1‑140 (MQ=255)                                                                                      gTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcgcg                                          >  1:1279397/1‑140 (MQ=255)                                                                                       tCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcgcgc                                         >  1:122728/1‑140 (MQ=255)                                                                                            gTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTtct                                    <  2:475063/140‑1 (MQ=255)                                                                                             tCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTtctc                                   >  2:177920/1‑140 (MQ=255)                                                                                               cGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGa                                 <  1:1179438/140‑1 (MQ=255)                                                                                                      gTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAg                          >  1:8584/1‑140 (MQ=255)                                                                                                      gTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAg                          >  2:1333310/1‑140 (MQ=255)                                                                                                      gTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAg                          >  1:926715/1‑140 (MQ=255)                                                                                                      gTCATGATGATGATGCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAg                          <  1:984835/140‑1 (MQ=255)                                                                                                         atgatgatgatgCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAt                       >  1:1388062/1‑140 (MQ=255)                                                                                                                  atgCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCtt              <  1:1333310/140‑1 (MQ=255)                                                                                                                   tgCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAg                          <  2:887804/127‑1 (MQ=255)                                                                                                                   tgCTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAg                          >  1:887804/1‑127 (MQ=255)                                                                                                                     cTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAg           >  1:101690/1‑140 (MQ=255)                                                                                                                     cTGTGATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAg           <  1:934896/140‑1 (MQ=255)                                                                                                                      tgtgATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGc          >  2:810163/1‑140 (MQ=255)                                                                                                                       gtgATAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCg         >  2:720120/1‑140 (MQ=255)                                                                                                                          aTAACGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCa      >  1:615510/1‑140 (MQ=255)                                                                                                                              cGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAAt  >  1:1000644/1‑140 (MQ=255)                                                                                                                              cGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAAt  >  1:1366388/1‑140 (MQ=255)                                                                                                                              cGCAGTTTGTCCTGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAAt  >  1:518282/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                GAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAAT  >  NC_000913/3878529‑3878793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑