Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,397,145	C→A	intergenic (+53/‑220)	insH‑4 → / → ynaJ	IS5 transposase and trans‑activator/DUF2534 domain‑containing protein YnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,397,145	0	C	A	100.0%	84.8 / NA	26	intergenic (+53/‑220)	insH‑4/ynaJ	IS5 transposase and trans‑activator/DUF2534 domain‑containing protein YnaJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/15);  total (11/15)

CCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTG  >  NC_000913/1397108‑1397280                                      |                                                                                                                                        ccTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAAt                                   <  1:223194/140‑1 (MQ=18)  cTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATc                                  <  1:891161/140‑1 (MQ=18)  cTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATc                                  >  1:887886/1‑140 (MQ=18)        tGGCGAAGAAACGGTCTAAATAGGCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCg                            <  1:1005494/140‑1 (MQ=255)        tGGCGAAGAAACGGTCTAAATAGGCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCg                            <  2:154137/140‑1 (MQ=255)        tGGCGAAGAAACGGTCTAAATAGGCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCg                            <  2:1060031/140‑1 (MQ=255)         ggCGAAGAAACGGTCTAAATAGGCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGt                           <  1:576918/140‑1 (MQ=255)          gCGAAGAAACGGTCTAAATAGGCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCg                            <  2:703672/138‑1 (MQ=38)          gCGAAGAAACGGTCTAAATAGGCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCg                            >  1:703672/1‑138 (MQ=255)                aaaCGGTCTAAATAGGCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGt                    <  1:200060/140‑1 (MQ=255)                     gTCTAAATAGGCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATc               <  2:887886/140‑1 (MQ=255)                     gTCTAAATAGGCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATc               <  2:702999/140‑1 (MQ=255)                     gTCTAAATAGGCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATc               <  1:557694/140‑1 (MQ=255)                      tCTAAATAGGCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATcc              >  2:1305167/1‑140 (MQ=255)                        tAAATAGGCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATc               >  2:128697/1‑137 (MQ=255)                        tAAATAGGCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATc               <  1:128697/137‑1 (MQ=255)                              ggCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAaga      >  2:805632/1‑140 (MQ=255)                               gCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAagag     >  1:451077/1‑140 (MQ=255)                               gCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAagag     >  1:378549/1‑140 (MQ=255)                               gCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAagag     >  2:411903/1‑140 (MQ=255)                               gCTGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAagag     >  1:1027727/1‑140 (MQ=255)                                cggATTAAAGGCATTTACGGAAGAAAAAACCGGCTCAAACATGAAAAAATG‑AATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAgaggt   <  2:508023/138‑1 (MQ=18)                                 tGATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAgaggt   <  1:304502/140‑1 (MQ=255)                                  gATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATcaacgca         <  1:841333/133‑7 (MQ=255)                                  gATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATcaacgca         >  2:841333/1‑127 (MQ=255)                                  gATTAAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAgaggtg  >  1:1253762/1‑140 (MQ=255)                                      |                                                                                                                                        CCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTG  >  NC_000913/1397108‑1397280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑