Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,678,604	G→T	A60S (GCA→TCA)	ydgH →	DUF1471 domain‑containing protein YdgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,678,604	0	G	T	100.0%	245.6 / NA	70	A60S (GCA→TCA)	ydgH	DUF1471 domain‑containing protein YdgH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (35/35);  total (35/35)

TGCTATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCGCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTAACG  >  NC_000913/1678468‑1678742                                                                                                                                         |                                                                                                                                           tGCTATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAg                                                                                                                                         <  2:1383124/140‑1 (MQ=255)   cTATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGAt                                                                                                                                       <  1:1338732/140‑1 (MQ=255)    tATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATa                                                                                                                                      <  2:600908/140‑1 (MQ=255)      tGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATaaa                                                                                                                                    >  1:137465/1‑140 (MQ=255)        gCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGACAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAaaga                                                                                                                                  >  1:5003/1‑140 (MQ=255)           ttCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAgg                                                                                                                               >  1:161275/1‑140 (MQ=255)             ctcCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTg                                                                                                                             >  2:627155/1‑140 (MQ=255)                cGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccg                                                                                                                          >  1:906847/1‑140 (MQ=255)                  ttAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                        <  2:978767/140‑1 (MQ=255)                  ttAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                        <  2:1204974/140‑1 (MQ=255)                    aaCGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCctc                                                                                                                      <  2:906847/140‑1 (MQ=255)                     aCGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCctct                                                                                                                     <  1:14886/140‑1 (MQ=255)                        cagcaACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                        >  2:877733/1‑134 (MQ=255)                        cagcaACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                        <  1:877733/134‑1 (MQ=255)                          gcaACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                        >  1:1313880/1‑132 (MQ=255)                          gcaACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                        <  2:1313880/132‑1 (MQ=255)                           caACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTAt                                                                                                               >  2:564046/1‑140 (MQ=255)                                gAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTAtgttgt                                                                                                          >  1:1182062/1‑140 (MQ=255)                                 aaCTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTc                                                                                                         >  2:369676/1‑140 (MQ=255)                                  aCTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCg                                                                                                        >  1:236100/1‑140 (MQ=255)                                   cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                       <  2:318885/140‑1 (MQ=255)                                   cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                       <  2:161275/140‑1 (MQ=255)                                   cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                       <  1:816612/140‑1 (MQ=255)                                   cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                       <  1:627155/140‑1 (MQ=255)                                         ccGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTc                                                                                                 >  1:1055450/1‑140 (MQ=255)                                             aGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGAt                                                                                             >  1:409952/1‑140 (MQ=255)                                                  gCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTgg                                                                                        >  1:940852/1‑140 (MQ=255)                                                   cGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGt                                                                                       <  2:474776/140‑1 (MQ=255)                                                    ggCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTa                                                                                      <  2:939809/140‑1 (MQ=255)                                                    ggCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTa                                                                                      <  2:441432/140‑1 (MQ=255)                                                            ttAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGt                                                                              <  2:236100/140‑1 (MQ=255)                                                               aaCCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAAc                                                                           <  2:208470/140‑1 (MQ=255)                                                                     ttGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAAc                                                                           >  1:1075873/1‑134 (MQ=255)                                                                     ttGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAAc                                                                           <  2:1075873/134‑1 (MQ=255)                                                                     ttGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCgt                                                                     <  1:791033/140‑1 (MQ=255)                                                                            tgtAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCgtg                                                                    <  1:610327/134‑1 (MQ=255)                                                                            tgtAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCgtg                                                                    >  2:610327/1‑134 (MQ=255)                                                                            tgtAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCg                                                              >  1:216039/1‑140 (MQ=255)                                                                            tgtAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCg                                                              <  1:369676/140‑1 (MQ=255)                                                                            tgtAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCg                                                              <  2:794505/140‑1 (MQ=255)                                                                            tgtAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGCGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCg                                                              >  1:1338600/1‑140 (MQ=255)                                                                                 tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                         <  2:216039/140‑1 (MQ=255)                                                                                 tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                         <  1:351044/140‑1 (MQ=255)                                                                                 tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                         <  2:1182062/140‑1 (MQ=255)                                                                                 tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                         <  1:837846/140‑1 (MQ=255)                                                                                  ggTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAcc                                                        >  1:1242804/1‑140 (MQ=255)                                                                                  ggTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAcc                                                        >  1:521109/1‑140 (MQ=255)                                                                                      aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCta                                                    >  1:1057450/1‑140 (MQ=255)                                                                                      aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACCGGCGTGTGGTCGCTGACCTCta                                                    >  1:443964/1‑140 (MQ=255)                                                                                        cGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCtata                                                  >  2:572518/1‑140 (MQ=255)                                                                                          gTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATaaa                                                >  1:236314/1‑140 (MQ=255)                                                                                           tCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAg                                               >  2:246527/1‑140 (MQ=255)                                                                                           tCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAg                                               >  1:1516789/1‑140 (MQ=255)                                                                                           tCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAg                                               >  1:509770/1‑140 (MQ=255)                                                                                                  aaTGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGc                                        >  1:280548/1‑140 (MQ=255)                                                                                                        aTTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaa                                  >  2:64320/1‑140 (MQ=255)                                                                                                         ttGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaaa                                 >  1:1100296/1‑140 (MQ=255)                                                                                                         ttGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaaa                                 >  2:627119/1‑140 (MQ=255)                                                                                                         ttGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaaa                                 >  2:1102423/1‑140 (MQ=255)                                                                                                            gCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCa                              <  1:365386/140‑1 (MQ=255)                                                                                                              gAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCaga                            <  1:961055/140‑1 (MQ=255)                                                                                                               aaGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCagaa                           >  1:1012082/1‑140 (MQ=255)                                                                                                                aGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCagaag                          >  2:1162397/1‑140 (MQ=255)                                                                                                                aGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCagaag                          <  2:521109/140‑1 (MQ=255)                                                                                                                  cGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAgaagaa                        <  2:137465/140‑1 (MQ=255)                                                                                                                       aatgCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAg                   <  2:1144584/137‑1 (MQ=255)                                                                                                                       aaaGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAg                   >  2:516701/1‑140 (MQ=255)                                                                                                                          gCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTaa                <  1:627119/140‑1 (MQ=255)                                                                                                                              tttCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgc            <  2:1114485/140‑1 (MQ=255)                                                                                                                              tttCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgc            <  1:285354/140‑1 (MQ=255)                                                                                                                              tttCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgc            <  2:409952/140‑1 (MQ=255)                                                                                                                                        cgcAGATAAAGAAGGTGCCGCCCCCCTTTCTGTTGTCGACACTTCTGATTTCGGCAACAGCGGTACCTGGCGTGTGGTCCCTGCCCTCTATAAAGCCCATGCTCAAAAAGCAGAACAAACAAGTAATCGCGTAACTAACg  <  2:1100296/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                           TGCTATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCGCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTAACG  >  NC_000913/1678468‑1678742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑