| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,825,061 | 0 | G | A | 30.2% | 70.5 / 15.7 | 43 | intergenic (‑124/+79) | ves/spy | HutD family protein Ves/ATP‑independent periplasmic chaperone |
| Reads supporting (aligned to +/- strand): ref base G (27/3); new base A (0/13); total (27/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.53e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCT > NC_000913/1824923‑1825195 | atCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGagaaaaga > 2:963947/1‑140 (MQ=255) ttATTCCATCCTCGCCCCCGGCCCTTTTTTTTCTTTGTTATCGTTCAGGTACCCGTGGATGGTTATCGGCAACTGGGGTGATTTACGTTGGGGGTGATCGGGTTTTTTTTAAAA‑TTGTTGGAGAAGAAAAAAAAAAAccg < 2:471606/139‑1 (MQ=255) tATTCCATCCTCGCTCCCGGCCTTTTTTTTTCATTGTTATCTTTCAGGTAACCGTGGATGGTTAGGGGCAACTGGGGTTTTTTACGTTAGTGGTGATCAGGTTTTTTTTACAA‑CTGTTTGAAAAGAAAAAAAAAAAccgc < 2:1652982/140‑1 (MQ=255) tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGa > 1:1872277/1‑140 (MQ=255) tACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCt > 1:78594/1‑140 (MQ=255) acccGCTCCCGCCCCTTTTTTTTTTTTTTTTTCGTTGAGGTAACGGGGGTTGGTTTTCGCCACCTGGGGTAATTTACGTTAGGGGTGATAGGGTTTTTTTTACAA‑TTGTTTGAGAAGAAAAAAGAAAACCGCCGATCCTg < 2:1055119/137‑1 (MQ=255) gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCa > 1:1394792/1‑140 (MQ=255) gCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCa > 1:1219917/1‑140 (MQ=255) cTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAc > 1:63319/1‑140 (MQ=255) cTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAc > 1:1609060/1‑140 (MQ=255) cTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAc > 1:252799/1‑140 (MQ=255) cccGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGTCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCg > 1:1776744/1‑140 (MQ=255) cccGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCg > 1:506259/1‑140 (MQ=255) ggCCCTTTTTTTTTATTTTTATCGTTCAGGTACCGGTGGTTGTTTATCGGCAACTGGGGTGTTTTACGTTAGTGGTGATCAGTTTTTTTTTACAA‑TTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAt < 2:1431792/140‑1 (MQ=255) cccGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTa > 2:1033520/1‑140 (MQ=255) ttCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc > 1:1431792/1‑140 (MQ=255) tCTTTTTATTTTTATCTTTCAGGTAACAGTGGTTGGTTCTCGGCATGTGGGGTGTTTTGCGTTGGTGGTGAGAGGTTTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAAAAAGCCGCCGATCCTGTCCACCGCATTACTGCa < 2:1219917/140‑1 (MQ=255) tCTTTTTATTGTTATCGTTGAGGTAACCGGGGATGGTAAGGGGCAGCTGGGGTGATTTGTGTTAGTGAGGCTGAGGTTTTTTCAACAC‑CTTTTAAAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCa < 2:63319/140‑1 (MQ=255) tCATTGTTATCGTTCAGGTACCGGTGGATGTTTATCGGCAACTGGGGTGATTTAGGTTAGTGGTGATCGGGCTTTTTTTACAT‑TTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTa < 2:506259/140‑1 (MQ=255) tGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg > 1:1473642/1‑140 (MQ=255) cAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg > 1:213078/1‑140 (MQ=255) aGGTAACCGTGGATGGTTATCGGCACCTGGGGTGTTTTACGTTAGTGGTGATCGGGTTTTTTTTACAA‑CTTTTTGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc < 2:1116728/140‑1 (MQ=255) aCCGTGGATGGTTATCGGCAACTCGGGCGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCt > 1:641723/1‑140 (MQ=255) cGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTa > 2:1500155/1‑140 (MQ=255) aCTGGGGTGATTTACTTTAGTGGTGATCGGTTTTTTTTAACAA‑TTGTTGGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGa < 2:305928/140‑1 (MQ=255) cTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat > 2:1053705/1‑140 (MQ=255) ggTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGattatt > 2:1901867/1‑140 (MQ=255) tttttCCGTTAGGGGTGATCAGGTTTTTTTTAAAT‑CTGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcag < 2:213078/138‑1 (MQ=255) ttttACTTTAGGGGTGATCGGGTTTTTTTTACAT‑TTGTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagc < 1:963947/139‑1 (MQ=255) tACGTTAGTGGTGATCAGGTTTTTTTTACAT‑TTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGt < 2:1065576/140‑1 (MQ=255) ttggtggtgATCGGTTTTTTTTTACAA‑CTGTTGGAAAAAAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCa < 1:1033520/137‑1 (MQ=255) ttAGTGGTGATCAGTTTTTTTTTACAA‑TTGTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCa < 2:1473642/140‑1 (MQ=255) ttAGTGGTGATCAGGTTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCa < 1:1389516/140‑1 (MQ=255) tggtgATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCa > 2:22026/1‑140 (MQ=255) ggtgATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTGGCTGAAAGATTATTATACAGTTTGAGGCAt > 2:370361/1‑140 (MQ=255) gATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt > 1:1163827/1‑140 (MQ=255) tCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCTGGCAtttttc > 2:1626264/1‑138 (MQ=255) tttttttttACAT‑TTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt < 1:1038001/138‑1 (MQ=255) ttttttttACAATTTGTTGGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt < 2:641723/139‑1 (MQ=255) tttttACAC‑TTTTTGGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGcc < 1:264352/140‑1 (MQ=255) cAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAAGGATTTTACCTTTTGCCGCTggt > 2:1132386/1‑139 (MQ=255) a‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACg > 2:1884934/1‑140 (MQ=255) a‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACg > 1:499475/1‑140 (MQ=255) tttttAAGATGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt < 1:370361/138‑1 (MQ=255) gTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTc > 2:389388/1‑140 (MQ=255) tttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt > 1:1276442/1‑140 (MQ=255) tttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt > 2:551039/1‑140 (MQ=255) tggagaaaaaaaAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTg < 2:1163827/138‑1 (MQ=255) agaaaaAAAAAATCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc < 1:970430/140‑1 (MQ=255) aaaaaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg < 1:1483562/137‑1 (MQ=255) gaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt > 2:1726423/1‑140 (MQ=255) | ATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAA‑CTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCT > NC_000913/1824923‑1825195 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |