BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,932,491	G→T	W392C (TGG→TGT)	kup →	K(+):H(+) symporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,932,491	0	G	T	99.4%	552.0 / ‑5.2	155	W392C (TGG→TGT)	kup	K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (75/79); minor base C (0/1); total (75/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 159 total aligned reads displayed.

ATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGA  >  NC_000913/3932355‑3932627

aTGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAAt                                                                                                                                       <  1:1708756/140‑1 (MQ=255)

aTGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAAt                                                                                                                                       <  1:1303113/140‑1 (MQ=255)

aTGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAAt                                                                                                                                       <  1:689743/140‑1 (MQ=255)

 tGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATa                                                                                                                                      >  2:1740510/1‑140 (MQ=255)

  gTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGtaataa                                                                                                                                     >  1:1305123/1‑140 (MQ=255)

    cgcgGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGCGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGt                                                                                                                                   >  1:494224/1‑140 (MQ=255)

      cgGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGATGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTAt                                                                                                                                 >  1:550473/1‑140 (MQ=255)

      cgGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTAt                                                                                                                                 >  1:716895/1‑140 (MQ=255)

      cgGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTAt                                                                                                                                 >  1:1486232/1‑140 (MQ=255)

      cgGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTAt                                                                                                                                 >  1:666170/1‑140 (MQ=255)

      cgGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTAt                                                                                                                                 >  1:1241324/1‑140 (MQ=255)

         tCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTAtttt                                                                                                                              >  1:600721/1‑140 (MQ=255)

          cGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTttg                                                                                                                             <  1:83047/140‑1 (MQ=255)

           gtgattgtgattGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTttgt                                                                                                                            >  1:1102070/1‑140 (MQ=255)

           gtgattgtgattGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTttgt                                                                                                                            <  2:1045593/140‑1 (MQ=255)

           gtgattgtgattGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTttgt                                                                                                                            <  2:1605494/140‑1 (MQ=255)

           gtgattgtgattGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTttgt                                                                                                                            >  2:167869/1‑140 (MQ=255)

           gtgattgtgattGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTttgt                                                                                                                            <  1:1843798/140‑1 (MQ=255)

           gtgattgtgattGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTttgt                                                                                                                            <  1:1842503/140‑1 (MQ=255)

           gtgattgtgattGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTttgt                                                                                                                            >  2:435319/1‑140 (MQ=255)

               ttgtgattgtCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTgcg                                                                                                                        >  1:1672857/1‑140 (MQ=255)

                 gtgattgtCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCt                                                                                                                      <  2:494224/140‑1 (MQ=255)

                  tgattgtCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTg                                                                                                                     <  1:1771026/140‑1 (MQ=255)

                   gattgtCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGa                                                                                                                    >  2:1430760/1‑140 (MQ=255)

                       gtCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCt                                                                                                                >  1:550991/1‑140 (MQ=255)

                        tCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTg                                                                                                               >  2:331815/1‑140 (MQ=255)

                        tCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTg                                                                                                               >  1:239229/1‑140 (MQ=255)

                            cTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTg                                                                                                           >  2:640444/1‑140 (MQ=255)

                            cTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTg                                                                                                           >  2:216421/1‑140 (MQ=255)

                            cTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTg                                                                                                           >  1:1791447/1‑140 (MQ=255)

                              ttGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCt                                                                                                         <  2:666170/140‑1 (MQ=255)

                              ttGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCt                                                                                                         >  1:209965/1‑140 (MQ=255)

                              ttGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCt                                                                                                         <  1:1430760/140‑1 (MQ=255)

                                 aGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTc                                                                                                      >  1:1217350/1‑140 (MQ=255)

                                     cTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCttt                                                                                                  <  2:239229/140‑1 (MQ=255)

                                      tCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTACTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTtg                                                                                                 >  1:1808776/1‑140 (MQ=255)

                                              ccTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGata                                                                                         <  2:701877/140‑1 (MQ=255)

                                                tGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGata                                                                                         >  2:131101/1‑138 (MQ=255)

                                                tGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATAtt                                                                                       >  1:1423149/1‑140 (MQ=255)

                                                tGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATAtt                                                                                       >  1:1716241/1‑140 (MQ=255)

                                                   cggcggcgTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCa                                                                                    <  2:765641/140‑1 (MQ=255)

                                                   cggcggcgTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCa                                                                                    <  1:865939/140‑1 (MQ=255)

                                                   cggcggcgTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCa                                                                                    <  1:406112/140‑1 (MQ=255)

                                                     gcggcgTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCAtt                                                                                  >  1:1328530/1‑140 (MQ=255)

                                                        gcgTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGtt                                                                               <  2:1241324/140‑1 (MQ=255)

                                                                   tGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAAcc                                                                    >  1:784293/1‑140 (MQ=255)

                                                                   tGCGGCGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAAcc                                                                    >  1:1908728/1‑140 (MQ=255)

                                                                      ggTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCg                                                                 <  2:784293/140‑1 (MQ=255)

                                                                      ggTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCg                                                                 <  2:577028/140‑1 (MQ=255)

                                                                       gTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGa                                                                <  1:239508/140‑1 (MQ=255)

                                                                          aCCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATaa                                                             >  1:744264/1‑140 (MQ=255)

                                                                           ccGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATaaa                                                            <  2:887947/140‑1 (MQ=255)

                                                                           ccGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATaaa                                                            <  2:504076/140‑1 (MQ=255)

                                                                                    tGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTcc                                                   >  2:784932/1‑140 (MQ=255)

                                                                                    tGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTcc                                                   >  1:2026246/1‑140 (MQ=255)

                                                                                      gTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGctct                                                     >  2:1558271/1‑136 (MQ=255)

                                                                                         cTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCcggcgg                                              >  2:856603/1‑140 (MQ=255)

                                                                                         cTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCcagcgg                                              >  1:1045404/1‑140 (MQ=255)

                                                                                           gACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCt                                            <  2:1285337/140‑1 (MQ=255)

                                                                                            aCGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGTTg                                           >  1:1395698/1‑140 (MQ=255)

                                                                                               tCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGtt                                        >  2:1587434/1‑140 (MQ=255)

                                                                                                 tATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGc                                      >  2:1156168/1‑140 (MQ=255)

                                                                                                      tctcGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTg                                 <  1:1156168/140‑1 (MQ=255)

                                                                                                      tctcGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTg                                 >  1:1210627/1‑140 (MQ=255)

                                                                                                        tcGACTACCGTGGCACGTCAGAGCTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAg                               <  2:1335365/140‑1 (MQ=255)

                                                                                                        tcGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAg                               >  1:369250/1‑140 (MQ=255)

                                                                                                        tcGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAg                               <  2:464828/140‑1 (MQ=255)

                                                                                                        tcGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAg                               >  1:616270/1‑140 (MQ=255)

                                                                                                        tcGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAg                               >  2:1411912/1‑140 (MQ=255)

                                                                                                        tcGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAg                               >  2:1376142/1‑140 (MQ=255)

                                                                                                        tcGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAg                               >  1:464828/1‑140 (MQ=255)

                                                                                                        tcGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAg                               >  1:1313522/1‑140 (MQ=255)

                                                                                                         cGACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGc                              >  1:879086/1‑140 (MQ=255)

                                                                                                          gACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAg                               >  1:482229/1‑138 (MQ=255)

                                                                                                          gACTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAg                               <  2:482229/138‑1 (MQ=255)

                                                                                                           aCTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCt                            <  2:1908728/140‑1 (MQ=255)

                                                                                                           aCTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCt                            <  2:1716241/140‑1 (MQ=255)

                                                                                                           aCTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCt                            <  2:167336/140‑1 (MQ=255)

                                                                                                           aCTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCt                            <  2:1505849/140‑1 (MQ=255)

                                                                                                           aCTACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCt                            <  2:1486232/140‑1 (MQ=255)

                                                                                                             tACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTg                                 <  2:1138683/133‑1 (MQ=255)

                                                                                                             tACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTg                                 >  1:1138683/1‑133 (MQ=255)

                                                                                                             tACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCg                          <  1:1587434/140‑1 (MQ=255)

                                                                                                             tACCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCg                          >  2:679944/1‑140 (MQ=255)

                                                                                                              aCCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCgg                         <  1:295586/140‑1 (MQ=255)

                                                                                                              aCCGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCgg                         <  1:1369089/140‑1 (MQ=255)

                                                                                                                cGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTg                                 <  2:1915556/130‑1 (MQ=255)

                                                                                                                cGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCt                            <  1:751795/135‑1 (MQ=255)

                                                                                                                cGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTa                       <  2:807015/140‑1 (MQ=255)

                                                                                                                cGTGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTa                       <  1:784932/140‑1 (MQ=255)

                                                                                                                  tGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACt                     >  2:169025/1‑140 (MQ=255)

                                                                                                                  tGGCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACt                     >  1:1383992/1‑140 (MQ=255)

                                                                                                                   ggCACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCC‑TTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACtgt                   <  2:716895/140‑1 (MQ=255)

                                                                                                                     cACGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACtgtg                  <  2:10081/140‑1 (MQ=255)

                                                                                                                       cGTCAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGAt                <  2:550991/140‑1 (MQ=255)

                                                                                                                          cAGAACTGGCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGtt             >  1:2064650/1‑140 (MQ=255)

                                                                                                                                  gCACTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGtga     >  1:1886751/1‑140 (MQ=255)

                                                                                                                                    aCTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTGCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGtgatg   >  2:2059760/1‑140 (MQ=255)

                                                                                                                                     cTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGtgatga  <  2:1210627/140‑1 (MQ=255)

                                                                                                                                     cTGTAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGtgatga  <  2:369250/140‑1 (MQ=255)

ATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCCAGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATTCTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATCCTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGA  >  NC_000913/3932355‑3932627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`