Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,070,417	G→T	intergenic (‑16/+98)	yihR ← / ← yihS	putative aldose 1‑epimerase YihR/sulfoquinovose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,070,417	0	G	T	100.0%	309.7 / NA	86	intergenic (‑16/+98)	yihR/yihS	putative aldose 1‑epimerase YihR/sulfoquinovose isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (54/32);  total (54/32)

AGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCG  >  NC_000913/4070282‑4070552                                                                                                                                        |                                                                                                                                         aGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGa                                                                                                                                      >  2:940256/1‑140 (MQ=255)    cAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGt                                                                                                                                   <  1:967784/140‑1 (MQ=255)    cAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGt                                                                                                                                   >  2:1878875/1‑140 (MQ=255)            cACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑a                                                                                                                          >  2:97874/1‑140 (MQ=255)            cACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑a                                                                                                                          >  1:708209/1‑140 (MQ=255)            cACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑a                                                                                                                          <  1:2064000/140‑1 (MQ=255)                gACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑aaaaa                                                                                                                      <  2:1592512/140‑1 (MQ=255)                 aCGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAAc                                                                                                                     >  1:413168/1‑140 (MQ=255)                 aCGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAAc                                                                                                                     >  2:1957104/1‑140 (MQ=255)                       acaATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCAcc                                                                                                               <  1:1847257/140‑1 (MQ=255)                       acaATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCAcc                                                                                                               >  1:1949575/1‑140 (MQ=255)                       acaATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCAcc                                                                                                               <  2:1352973/140‑1 (MQ=255)                        caATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCg                                                                                                              <  1:1770455/140‑1 (MQ=255)                           tGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGAc                                                                                                           <  1:1878875/140‑1 (MQ=255)                            ggTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACg                                                                                                          <  2:714070/140‑1 (MQ=255)                            ggTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACg                                                                                                          <  2:1631039/140‑1 (MQ=255)                            ggTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACg                                                                                                          <  2:196060/140‑1 (MQ=255)                             gTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACgg                                                                                                         >  1:54244/1‑140 (MQ=255)                              tCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGt                                                                                                        <  2:285285/140‑1 (MQ=255)                               cgcgTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTc                                                                                                       >  1:1531156/1‑140 (MQ=255)                               cgcgTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTc                                                                                                       >  1:1616680/1‑140 (MQ=255)                               cgcgTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTc                                                                                                       >  1:519067/1‑140 (MQ=255)                                gcgTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTcc                                                                                                      >  2:520197/1‑140 (MQ=255)                                 cgTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTccc                                                                                                     <  1:1579796/140‑1 (MQ=255)                                 cgTGATAATCCCCCGCGGCAAGTGAAACGATCTGTCCACTGCAATGCATATTGTTGCATTGCATACAAGGCACTTTAATTAACGTGATCCAGCCTTCCGTGATCCTGATGTCAAACCC‑AAAAAGTACACCGGACGGTccc                                                                                                     >  2:1776607/1‑140 (MQ=255)                                     aTAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATGTTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCg                                                                                                 <  1:1852673/140‑1 (MQ=255)                                     aTAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCg                                                                                                 <  1:831142/140‑1 (MQ=255)                                       aaTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGcc                                                                                               >  2:1733552/1‑140 (MQ=255)                                        aTCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGccc                                                                                              <  2:1531156/140‑1 (MQ=255)                                           ccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCtt                                                                                           >  1:1952055/1‑140 (MQ=255)                                           ccccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCtt                                                                                           <  1:1701178/140‑1 (MQ=255)                                            cccGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCttt                                                                                          >  1:1960863/1‑140 (MQ=255)                                              cgcgGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTgg                                                                                        >  1:1058753/1‑140 (MQ=255)                                                cgGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTgggg                                                                                      >  1:1357878/1‑140 (MQ=255)                                                 ggCAAGTGAGACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                     <  2:522740/140‑1 (MQ=255)                                                 ggCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                     <  2:54244/140‑1 (MQ=255)                                                 ggCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                     <  2:1949575/140‑1 (MQ=255)                                                 ggCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                     <  2:717794/140‑1 (MQ=255)                                                 ggCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                     <  2:1617794/140‑1 (MQ=255)                                                 ggCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                     <  1:195149/140‑1 (MQ=255)                                                 ggCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                     <  2:519067/140‑1 (MQ=255)                                                 ggCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                     <  1:940256/140‑1 (MQ=255)                                                 ggCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                     <  2:1058753/140‑1 (MQ=255)                                                 ggCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGga                                                                                     <  2:2030156/140‑1 (MQ=255)                                                 ggCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACAGGACGGTCCCCTCGCCCCTTTGGGga                                                                                     <  1:1313263/140‑1 (MQ=255)                                                   cAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGgaga                                                                                   <  2:863201/140‑1 (MQ=255)                                                   cAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGgaga                                                                                   <  2:929518/140‑1 (MQ=255)                                                   cAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGgaga                                                                                   <  2:1960863/140‑1 (MQ=255)                                                    aaGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGagag                                                                                  <  1:97874/140‑1 (MQ=255)                                                              gTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGgag                                                                        >  1:441480/1‑140 (MQ=255)                                                                 tGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAgg                                                                     >  1:520559/1‑140 (MQ=255)                                                                        tGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAAc                                                              >  2:1091580/1‑140 (MQ=255)                                                                        tGCAATGCATATTGGTGATTTGCATACAACGCCCCTTAATTAACAACATACAGCCTCCCGTGAGGCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCCTTGGGGAGCGGGTTAGGCAGAGGGGAAAAc                                                              >  2:1505153/1‑140 (MQ=39)                                                                         gCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAAcc                                                             >  1:273529/1‑140 (MQ=255)                                                                         gCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAAcc                                                             >  2:333832/1‑140 (MQ=255)                                                                             tGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCa                                                         >  2:1268926/1‑140 (MQ=255)                                                                             tGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCa                                                         >  1:1697062/1‑140 (MQ=255)                                                                             tGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCa                                                         >  1:1662549/1‑140 (MQ=255)                                                                                  atTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTa                                                    >  1:68098/1‑140 (MQ=255)                                                                                  atTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTa                                                    >  1:1673914/1‑140 (MQ=255)                                                                                    tGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCGGCACCCTAtt                                                  >  2:829978/1‑140 (MQ=255)                                                                                    tGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTAtt                                                  >  2:1956165/1‑140 (MQ=255)                                                                                      gTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTAtttt                                                >  1:1999161/1‑140 (MQ=255)                                                                                         aTTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAg                                             >  2:448346/1‑140 (MQ=255)                                                                                         aTTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAg                                             >  2:1876497/1‑140 (MQ=255)                                                                                         aTTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAg                                             >  2:1029328/1‑140 (MQ=255)                                                                                           ttGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGAt                                           >  1:1992484/1‑140 (MQ=255)                                                                                                tACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGtt                                      >  1:788611/1‑140 (MQ=255)                                                                                                    agcccccTTATTAAACGACATACGGCCCCCGGTGGGTTTGATG‑CAAACCCAAAAAACTGCCCCGGCCGCCCCCCCCCCCCTTTGGGGGAGGGGGTGGGGGGGGGGGGAAAACCCGCACCCTATTTTCAGATCAGTTAttt                                  <  2:413168/135‑1 (MQ=255)                                                                                                    aGGCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTAttt                                  >  1:200796/1‑140 (MQ=255)                                                                                                     ggCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTc                                 >  2:1618472/1‑140 (MQ=255)                                                                                                     ggCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTc                                 >  2:1329971/1‑140 (MQ=255)                                                                                                     ggCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTc                                 >  1:377353/1‑140 (MQ=255)                                                                                                     ggCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTc                                 >  2:408723/1‑140 (MQ=255)                                                                                                     ggCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGATCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGATAGGAAAACCCGCACCCTATAGTCAGATCAGTTATTTc                                 >  2:1407879/1‑140 (MQ=255)                                                                                                      gCACCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCg                                >  1:382845/1‑140 (MQ=255)                                                                                                        aCCTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCa                              >  2:191758/1‑140 (MQ=255)                                                                                                          cTTAATTAACGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCAtt                            >  1:1363347/1‑140 (MQ=255)                                                                                                           ttaattaaCGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTa                           >  2:709786/1‑140 (MQ=255)                                                                                                                   cGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcag                   >  1:1225054/1‑140 (MQ=255)                                                                                                                   cGACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcag                   >  1:586652/1‑140 (MQ=255)                                                                                                                    gACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGCGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagc                  >  2:2048771/1‑140 (MQ=255)                                                                                                                    gACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagc                  >  1:2004544/1‑140 (MQ=255)                                                                                                                    gACATACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagc                  >  1:1089483/1‑140 (MQ=255)                                                                                                                    gACATACAGACTCCCGTGAGTCTGATGTCAAACCT‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagc                  >  2:890541/1‑140 (MQ=255)                                                                                                                        tACAGCCTCCCGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGACCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGAc              >  1:1350049/1‑140 (MQ=255)                                                                                                                                cccGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCa      >  2:863309/1‑140 (MQ=255)                                                                                                                                cccGTGAGTCTGATGCCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGACCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCa      <  2:1350049/140‑1 (MQ=255)                                                                                                                                  cGTGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAAAGGGGAAAACCCGCACCCTTTTTTCAGATCAGTTATTTCGCATTAAAATCCAGCAGACCCGCTGCAAc    >  1:1032112/1‑140 (MQ=255)                                                                                                                                    tGAGTCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCg  >  1:1803741/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         AGCCAGACCAGCACCGACGGTCACAATGGTCGCGTGATAATCCCCCGCGGCAAGTGAAACGGTCTGTCCACTGCAATGCATATTGGTGATTTGCATACAAGGCACCTTAATTAACGACATACAGCCTCCCGTGAGGCTGATGTCAAACCC‑AAAAACTGCACCGGACGGTCCCCTCGCCCCTTTGGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCCGCTGCAACCG  >  NC_000913/4070282‑4070552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑