Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,444	0	T	A	24.1%	110.2 / 14.6	54	intergenic (‑188/‑19)	ttdR/ttdA	DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (7/34);  new base A (13/0);  total (20/34)
Fisher's exact test for biased strand distribution p-value = 7.00e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206307‑3206583                                                                                                                                          |                                                                                                                                              ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                                             <  1:1747165/140‑1 (MQ=255)         tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAACaaaaaaaa                                                                                                                                     >  2:250416/1‑137 (MQ=255)           ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTAATTTTTTCAGAcaaaaacaaaaaacaga                                                                                                                                   >  1:1282787/1‑135 (MQ=255)           ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTTGACAaaaaaaaaaaaagga                                                                                                                                   >  2:1561278/1‑135 (MQ=255)           ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAaaaaaaaaaaaagga                                                                                                                                   >  1:722904/1‑135 (MQ=255)           ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGa                                                                                                                                   >  1:942224/1‑140 (MQ=255)           ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAACAggg                                                                                                                                   >  2:570689/1‑139 (MQ=255)            tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                  <  1:1551057/140‑1 (MQ=255)            tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                  <  1:434195/140‑1 (MQ=255)                gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAAACAAAAAAAAAAAATGGAGATGc                                                                                                                              >  2:216922/1‑140 (MQ=255)                 cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAAAGGAGTTGcc                                                                                                                             >  1:624720/1‑140 (MQ=255)                 cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGcc                                                                                                                             <  1:1224440/140‑1 (MQ=255)                 cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGcc                                                                                                                             <  1:1384818/140‑1 (MQ=255)                   cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAACAAAAAATGGAGTTggcct                                                                                                                           >  2:1384818/1‑136 (MQ=255)                   cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATGGAGGTGGCat                                                                                                                           >  2:1722583/1‑140 (MQ=255)                     atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGCCatgg                                                                                                                         >  2:1224440/1‑139 (MQ=255)                       attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatgatg                                                                                                                       <  2:1637202/140‑1 (MQ=255)                         tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                                     <  1:1722133/140‑1 (MQ=255)                            ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                  <  1:16222/140‑1 (MQ=255)                            ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                  <  2:838485/140‑1 (MQ=255)                                tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCTAACAAATAAAAAAAATGGAGGTGGCATGAAGAGGGGAAga                                                                                                              >  1:1632863/1‑139 (MQ=255)                                tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTCGACAAAAACAAAAAATGGAGGTGCCATGATGAGCGAAAga                                                                                                              >  1:1277278/1‑139 (MQ=255)                                tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTACACAAAAAAAAAAAATAGAGGTGTCATGATGAGGGaaaaa                                                                                                              >  2:385326/1‑138 (MQ=255)                                 aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGGCATTATGAGCGAAAGta                                                                                                             >  2:648062/1‑140 (MQ=255)                                  ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTTGACAAAAAAAAAAAAAGGAGGTGCCCTGATGAGCGaaaaaaa                                                                                                            >  2:1722133/1‑136 (MQ=255)                                  ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAAAGGAGGTGCCATGATGAGGGaaaagaa                                                                                                            >  2:330657/1‑136 (MQ=255)                                   tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                           <  1:1337222/140‑1 (MQ=255)                                          ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc                                                                                                    <  1:319419/140‑1 (MQ=255)                                          ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc                                                                                                    <  1:1561278/140‑1 (MQ=255)                                           tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGTTGCCCTGATGAGGGAAAATAAAAAGaaaaa                                                                                                   >  1:159622/1‑138 (MQ=255)                                              ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                <  1:1672309/140‑1 (MQ=255)                                              ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                <  2:942224/140‑1 (MQ=255)                                              ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                                <  2:620106/140‑1 (MQ=255)                                               tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                               <  1:216922/140‑1 (MQ=255)                                               tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                               <  1:1292198/140‑1 (MQ=255)                                                    aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                          <  2:1440320/140‑1 (MQ=255)                                                       tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCACACAAAAAAAAAAAATAGGGATGTCATGATGGGCGGAAGAAAAAAAGAAAAAGCAGTGAATaa                                                                                       >  2:226303/1‑140 (MQ=255)                                                        gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg                                                                                      <  2:73067/140‑1 (MQ=255)                                                        gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg                                                                                      <  2:908089/140‑1 (MQ=255)                                                        gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg                                                                                      <  1:226303/140‑1 (MQ=255)                                                          tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGtt                                                                                    <  1:230979/140‑1 (MQ=255)                                                                   atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCCTGATGAGGGGAAAAAATAAAAAAAAAGCAGAGAAAAAAATTAC‑‑Agagaa                                                                         >  2:1219877/1‑139 (MQ=255)                                                                   atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAACAAAAAAAAAAAAAGGAGTTTGCCTTATGGGCGAAAGAAATAAACAACAGGCAAGGAAAAAGTTGGA‑‑AGAGAt                                                                         >  2:219130/1‑140 (MQ=255)                                                                     tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑‑AGAGATTg                                                                       <  2:1696142/140‑1 (MQ=255)                                                                        cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑‑AGAGATTGTCg                                                                    <  1:455364/140‑1 (MQ=255)                                                                             gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑‑AGAGATTGTCGCTAAc                                                               <  1:747964/140‑1 (MQ=255)                                                                              cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGGCCTGATGAGCGAAAGTAAAAAAAAAAAAGCAGGGAAAAAGTTGAA‑‑AGAGATTGTCGCTAACt                                                              >  2:1213289/1‑140 (MQ=255)                                                                                      ataAAACCAAATTTTTAGTGGTAGTTTGTGGCGGTAAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATCACGACCGAAAGAAATAAGCAACAGACAGTGAATAAGTTGAA‑‑AGAGTTTGTCGCTACTTTTACCGcc                                                      <  2:1277278/140‑1 (MQ=255)                                                                                       aaaaaCCACATTTTTAGTGGTAGTTTGTGGGGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAGATTGTCGCTAACTTTACCGc                                                       <  2:722904/139‑1 (MQ=255)                                                                                       aaaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑‑AGAGATTGTCGCTAACTTTACCGCCa                                                     <  1:1213289/139‑1 (MQ=255)                                                                                        aaaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTCCACAAAAAAAAAAAAAGGAGTTGGCATGTATAGGGAAAGTAAAAAACAAAAAGCAGAGAAAAAAATGGA‑‑GAAAATTGTCGCTAACTTTACccccca                                                    >  2:974518/1‑138 (MQ=255)                                                                                         aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑‑AGAGATTGTCGCTAACTTTACCGCCATg                                                   <  1:659869/140‑1 (MQ=255)                                                                                           aCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑‑AGAGATTGTCGCTAACTTTACCGCCATGAt                                                 <  2:1067313/140‑1 (MQ=255)                                                                                                       ggggTATTTTGTGGGGGTGAATTTTTCCAGAAAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGAC‑‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt                                     <  2:478023/138‑1 (MQ=255)                                                                                                          gTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAAAGGGGGTGGCATGATGAGGGAAAGTAATAAAAAACAAGCAGGGAATAAGTTGAA‑‑AAAGATTTTTGCTAAATTTACCGCCATGATTTTTACCCCCATGcc                                  >  2:201735/1‑140 (MQ=255)                                                                                                          gTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGAC‑‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGcc                                  <  2:1645918/140‑1 (MQ=255)                                                                                                          gTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGcc                                  <  1:313782/140‑1 (MQ=255)                                                                                                                   ggCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTGGAC‑‑AGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                         <  2:289283/140‑1 (MQ=255)                                                                                                                         gAATTTTTCCAGACAAATACAAAACCGGGAGTTGCAATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                   <  1:1705411/140‑1 (MQ=255)                                                                                                                                   aGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa         <  1:940877/140‑1 (MQ=255)                                                                                                                                   aGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGAAGTGAAAAAGTTGAC‑‑AGAGTTTGTCGCTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa         <  1:1149101/140‑1 (MQ=255)                                                                                                                                   aGAAAAATACAAAAACTGGAGTTCCCATGATGGGCGAAAGTAATAAGAACAAGGCAGTAAATAAGTTTAC‑‑AGAGATTGTGGCAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa         <  2:488646/140‑1 (MQ=255)                                                                                                                                    gACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc        >  2:1535243/1‑140 (MQ=255)                                                                                                                                      cAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg      >  1:488646/1‑140 (MQ=255)                                                                                                                                       aaaTACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc     >  2:1338421/1‑140 (MQ=255)                                                                                                                                       aaaTACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc     >  1:533282/1‑140 (MQ=255)                                                                                                                                         aTACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa   >  1:448558/1‑140 (MQ=255)                                                                                                                                          tACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  >  2:1154535/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                              CCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206307‑3206583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑