Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,223,5930TG21.6% 212.2 / 11.5 88K12N (AAA→AAC) iclRDNA‑binding transcriptional repressor IclR
Reads supporting (aligned to +/- strand):  ref base T (11/58);  new base G (19/0);  total (30/58)
Fisher's exact test for biased strand distribution p-value = 6.13e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 108 total aligned reads displayed.

GAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTC  >  NC_000913/4223456‑4223731
                                                                                                                                         |                                                                                                                                            
gAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGttt                                                                                                                                            >  1:1265599/1‑140 (MQ=255)
 aaTTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGtttt                                                                                                                                           <  1:1068004/140‑1 (MQ=255)
   ttGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGgttttt                                                                                                                                         >  2:1017738/1‑138 (MQ=255)
        tAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGgttttttcccc                                                                                                                                    >  2:1716992/1‑133 (MQ=255)
          aCCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TTCcgcgg                                                                                                                                 >  2:1786887/1‑139 (MQ=255)
           cccGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGt‑t                                                                                                                               <  2:60312/140‑1 (MQ=255)
           cccGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTC‑TGCCGCGt‑t                                                                                                                               >  1:60312/1‑140 (MQ=255)
             cGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTc                                                                                                                             <  2:1581938/140‑1 (MQ=255)
                cTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TTCCGCGT‑TTcgcg                                                                                                                          >  2:1009864/1‑140 (MQ=255)
                cTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTC‑TGCCGCGG‑TTtgcg                                                                                                                          >  1:744403/1‑140 (MQ=255)
                    ttgCGCCAGTTCCGTGAGTGCCACACTGTCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGaa                                                                                                                      <  2:155256/140‑1 (MQ=255)
                    ttgCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGaa                                                                                                                      <  1:965976/140‑1 (MQ=255)
                    ttgCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGaa                                                                                                                      <  1:1720802/140‑1 (MQ=255)
                     tgCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAAt                                                                                                                     >  1:94673/1‑140 (MQ=255)
                     tgCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TGCCGCGG‑TTCGCggggaa                                                                                                                     >  2:1353323/1‑137 (MQ=255)
                      gcgcCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TTTCCCGT‑TTTGCGGGGATg                                                                                                                    >  2:1736362/1‑140 (MQ=255)
                         gacagtCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGt                                                                                                                 <  1:451692/135‑1 (MQ=255)
                         ccAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATgggg                                                                                                                 >  1:796730/1‑139 (MQ=255)
                         ccAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGt                                                                                                                 <  1:1716992/140‑1 (MQ=255)
                         ccAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGt                                                                                                                 <  1:959746/140‑1 (MQ=255)
                           aGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGc                                                                                                               <  1:7669/140‑1 (MQ=255)
                             ttCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTT‑TGCCGCGT‑TTCGCGGGGATGGGGGCgg                                                                                                             >  2:369794/1‑139 (MQ=255)
                                   gAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGa                                                                                                       <  1:189668/140‑1 (MQ=255)
                                    aGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGATTGGGTGCGACCATGAc                                                                                                      <  1:238364/140‑1 (MQ=255)
                                      tGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTT‑TGGCGCGT‑TTCGCCGGGATGGGGGGGAcccttaccg                                                                                                    >  2:1685884/1‑133 (MQ=255)
                                      tGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAg                                                                                                    <  1:1084433/140‑1 (MQ=255)
                                      tGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TTCCGCGG‑TTCGCGGGGATGGGGGGGGCCATGACAg                                                                                                    >  2:230577/1‑140 (MQ=255)
                                       gCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGt                                                                                                   >  1:1558038/1‑140 (MQ=255)
                                         cacaCTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTC‑TGCCGCGT‑TTCGCGGGAATGGGGGGGGCCATGACAGgct                                                                                                 >  1:14009/1‑140 (MQ=255)
                                           cacTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTctct                                                                                               <  1:913595/140‑1 (MQ=255)
                                           cacTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTctct                                                                                               <  2:1191823/140‑1 (MQ=255)
                                           cacTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTctct                                                                                               <  2:1558038/140‑1 (MQ=255)
                                           cacTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTctct                                                                                               <  1:1180513/140‑1 (MQ=255)
                                              tGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCtttt                                                                                            >  1:116566/1‑140 (MQ=255)
                                                ccATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTT‑TCCCGCGG‑TTCCGGGGGATGGGGGGGACCCGAAAAGGCTCtttttt                                                                                          >  2:1274516/1‑140 (MQ=255)
                                                ccATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCtttttt                                                                                          >  2:183810/1‑140 (MQ=255)
                                                ccATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCtttttt                                                                                          <  1:183810/140‑1 (MQ=255)
                                                   ttGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTC‑TTCCGCGG‑TTCGCGGGGAAGGGGGCGGCCAGGACAGTCTCttttttttt                                                                                       >  1:264590/1‑137 (MQ=255)
                                                      gATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTGTTC‑TGTCGCGC‑TTCTCGGGCAGGGGGGTGCCCATTACTGATTCTTTTTTTTTTAt                                                                                    >  1:641183/1‑140 (MQ=255)
                                                          cGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TTCCGCGT‑TTTGCGGGGATGGGGGGGGCCCTGACAGGCTCTTTTTTTTTTTTCGTg                                                                                >  2:900667/1‑140 (MQ=255)
                                                          cGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TTCCCCGG‑TTCGCGGGGATGGGGGGGGCCCCGACAGTCTCTTTTTTTTTTATCGTg                                                                                >  2:1483654/1‑140 (MQ=255)
                                                          cGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TGCCGCGT‑TTCGCGGGGAGGGGGGGGGGCATGACAGTCTCTTTTTTTTGTATCggg                                                                                >  2:1815190/1‑138 (MQ=255)
                                                          cGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TGCCGCGG‑TTTGCGGGGAAGGGGGGGGCCATGACAGTCTCTTTTTTTTGTATtgtg                                                                                >  2:1697018/1‑140 (MQ=255)
                                                          cGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TGCCGCGG‑TTTGCGGGAAAGGGGGCGACCATGACAGTCTCTTTTTTTTGTATCGTg                                                                                >  2:1095394/1‑140 (MQ=255)
                                                          cGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTC‑TTCCGCGG‑TTCGCGGGGATGGGGGCGGCCATGACAGTCTCTTTTTTTTGTATCGTg                                                                                >  2:481284/1‑140 (MQ=255)
                                                             cAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TGCCGCGT‑TTCGCGGGGATGGGGGCGGCCATGACAGTCTCTTTTTTTTTTATCGTggga                                                                             >  2:264519/1‑138 (MQ=255)
                                                              aaTCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGaaa                                                                            <  1:1009864/140‑1 (MQ=255)
                                                                     tCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGG‑TTCGCGGGGATGGGGGCGACCATGACAGTCTCTTTTTTTTGTATCGTGGAAAtcatttt                                                                     >  1:273669/1‑140 (MQ=255)
                                                                          tAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGTAGCGGTGGCAACGGCGGGTTTTCTTTCCCGGT‑TTGACGGAAAGGGGGGCG‑CCATGCCAGCATCATTTTTTTTTATTGTTGAGAtcacttttcttt                                                                >  2:368042/1‑135 (MQ=255)
                                                                          tAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TTCCGCGG‑TTTGCGGGGATGGGTGGGACCATGACAGTCTCTTTTTTTTTTTTCGTGGGAAtcatttttattt                                                                >  2:1444499/1‑140 (MQ=255)
                                                                          tAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TGCCGCGT‑TTTGCGGGGAAGGGGGCGGCCCTGACAGGCTCTTTTTTTTGTTTTGTGGAAAtaatttttattt                                                                >  2:764290/1‑140 (MQ=255)
                                                                           aaTTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATcattttcatttt                                                               <  1:188445/140‑1 (MQ=255)
                                                                               tCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGGATGGGGGCGGCCCTGACAGTCTCTTTTTTTTGTATCGTGGGAATCATTTTTAtttttttt                                                           >  2:1379447/1‑140 (MQ=255)
                                                                               tCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTC‑TTCCCCGT‑TTTGCGGGAATGGGGGCGACCATGACAAACTCTTTTTTTTGTTTCGTGGGAATCATTTTTAtttttttt                                                           >  2:1304094/1‑140 (MQ=255)
                                                                                 aGGCCATGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTT‑TGTCGCGTCTTGGGGGGGAT‑AGTGGGGACAGGACACAATCTTTTTTTTGTCTTGTGCAAATCTttttcttttttctttt                                                         >  2:590761/1‑138 (MQ=255)
                                                                                 aGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTT‑TGCCGCGT‑TTCGCGGGGATGGGGGCGACCCTGACAGGCTCTTTTTTTTGTATCGTGGAAATCATTTTCATTTTTTTTGt                                                         >  1:1710712/1‑140 (MQ=255)
                                                                                 aGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTC‑TGCCGCGG‑TTTGCGGGGGTGGGGGGGCGCCTGGAAGGCCCTTTTTTTTTTATCGTGGGAAACATTTTCCtttttttttt                                                         >  2:1472837/1‑138 (MQ=255)
                                                                                    ccACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TTCCGCGT‑TTCGCGGGGAAGGGGGCGACCCTGACAGTCTCTTTTTTTTTTATCGTGGGAATCATTTTTATtttttttttttg                                                      >  2:287320/1‑138 (MQ=255)
                                                                                     cACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGc                                                     <  1:900667/140‑1 (MQ=255)
                                                                                     cACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGc                                                     <  1:1815190/140‑1 (MQ=255)
                                                                                     cACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGc                                                     <  1:1353323/140‑1 (MQ=255)
                                                                                      aCGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TGCCGCGT‑TTCGCGGGGGTGGGGGGGACCATGACAGGCTCTTTTTTTTGTTTCGGGGGAATCATTTTCTTTTTTTTTGTTAGCt                                                    >  2:335594/1‑140 (MQ=255)
                                                                                       cgcgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTa                                                   <  1:1657225/140‑1 (MQ=255)
                                                                                         cgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAAt                                                 <  1:168949/140‑1 (MQ=255)
                                                                                         cgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAAt                                                 <  1:1472837/140‑1 (MQ=255)
                                                                                          gTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATg                                                <  1:1274516/140‑1 (MQ=255)
                                                                                           ttAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGc                                               <  1:369794/140‑1 (MQ=255)
                                                                                              aaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt                                            <  1:1685884/140‑1 (MQ=255)
                                                                                              aaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt                                            <  1:1138953/140‑1 (MQ=255)
                                                                                              aaGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAAt                                            <  2:1713479/140‑1 (MQ=255)
                                                                                                gACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAg                                          <  1:1483654/140‑1 (MQ=255)
                                                                                                 aCTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGTGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGt                                         <  2:793434/140‑1 (MQ=255)
                                                                                                 aCTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGt                                         <  2:116566/140‑1 (MQ=255)
                                                                                                   tGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTa                                       <  2:381460/140‑1 (MQ=255)
                                                                                                     aaCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TGCCGCGT‑TTCGCGGGGATGGGGGCGACCATGACAGTCTCTTTTTTTTTTATCGTGGGAATCATTTTCATTTTTTTTTTTTGCTAATGCAATAGTTact                                     >  2:857564/1‑140 (MQ=255)
                                                                                                      aCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactg                                    <  1:1786887/140‑1 (MQ=255)
                                                                                                      aCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TGCCGCGT‑TTCGCGGGGATGGGGGCGACCATGACAGTCTCTTTTTTTTGTATCGTGGAAATCATTTTCATTTTTTTTTTTTGCTAATGCAATAGTTactg                                    >  1:419126/1‑140 (MQ=255)
                                                                                                        cTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactgaa                                  <  2:796730/140‑1 (MQ=255)
                                                                                                        cTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTactgaa                                  <  2:1265599/140‑1 (MQ=255)
                                                                                                          gTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TGCCGCGG‑TTCGCGGGGATGGGGGCGACCCCGACAGTCTCTTTTTTTTTTATTGTGGAAATCATTTTTTTTTTTTTTTTTTGCTAATTCAATAATTacttaaca                                >  2:475260/1‑139 (MQ=255)
                                                                                                           tCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTT‑TGCCGCGT‑TTTGCGGGGATGGGGGCGACCCTGACAGTCTCTTTTTTTTTTATCGTGGGAATTATTTTTATTTTTTTTTTTTGCTAATGCAATAGTTactgaaatt                               >  2:1702844/1‑137 (MQ=255)
                                                                                                               gTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TGCCGCGT‑TTCGCGGGGATGGGTGCGACCATGACAGTCTCTTTTTTTTGTATCGTGGGAAACATTTTTATTTTTTTTTTTTGCTAATGCAATAGTTACTGAACTGATcc                           >  1:1226470/1‑140 (MQ=255)
                                                                                                               gTCGCTGGTGCGGTGGCAACGGCGGGGTTTT‑TGCCGCGG‑TTCGCGGGGATGGGGGGGACCATGACAGTCTCTTTTTTTTTTATCGTGGGAATCATTTTCATTTTTTTTTTTTGCTAATGCAATAGTTACTGGAATGATcc                           >  2:1324655/1‑140 (MQ=255)
                                                                                                                tCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCg                          <  2:419126/140‑1 (MQ=255)
                                                                                                                 cGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGa                         <  1:287320/140‑1 (MQ=255)
                                                                                                                 cGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGa                         <  1:216566/140‑1 (MQ=255)
                                                                                                                 cGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGa                         <  2:744403/140‑1 (MQ=255)
                                                                                                                 cGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGa                         <  1:368042/140‑1 (MQ=255)
                                                                                                                 cGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGa                         <  1:1304094/140‑1 (MQ=255)
                                                                                                                     ggTGCGGTGGCAACGGCGGGTTTTT‑TTCCGCGT‑TTCGCGGGGATGGGGGCGGCCCTGACAGTCTCTTTTTTTTTTATTGTGGGAATCATTTTCATTTTTTTTTTTTGCTAATGCAATAGTTACTGAAATGATCCGATGAg                     >  2:1533468/1‑140 (MQ=255)
                                                                                                                      gTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGt                    <  2:641183/140‑1 (MQ=255)
                                                                                                                        gCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTa                  <  1:1533468/140‑1 (MQ=255)
                                                                                                                          ggTGGCAACGGCGGGGTTTT‑TGCCGCGG‑TTTGCGGGGATGGGGGCGGCCATGACAGTCTCTTTTTTTTGTATCGTGGGAATCATTTTTATTTTTTTTTTTTGCTAATTCAATAGTTACTTAAATGATCCGATGAGTTAAt                >  2:1067378/1‑140 (MQ=255)
                                                                                                                           gTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATg               <  1:1017738/140‑1 (MQ=255)
                                                                                                                            tGGCAACGGCGGGGTTTT‑TGCCGCGG‑TTTGCGGGGATGGGGGGGGCCCTGACAGGCTCTTTTTTTTGTTTTGTGGGAATCATTTTTTTTTTTTTTGTTTGCTAATGCAATAGTTACTGAACTGATCCGATGAGGTTATGt              >  2:1198214/1‑140 (MQ=255)
                                                                                                                                 aCGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAAc         <  2:553200/140‑1 (MQ=255)
                                                                                                                                   ggcggGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACaa       <  1:481284/140‑1 (MQ=255)
                                                                                                                                        gTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAAtctc  <  1:1095394/140‑1 (MQ=255)
                                                                                                                                        gTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAAtctc  <  1:1444499/140‑1 (MQ=255)
                                                                                                                                        gTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAAtctc  <  2:1710712/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                            
GAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTC‑TGCCGCGT‑TTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTTCATTTTTATTGTTAGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTC  >  NC_000913/4223456‑4223731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑