Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,678,604	G→T	A60S (GCA→TCA)	ydgH →	DUF1471 domain‑containing protein YdgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,678,604	0	G	T	100.0%	319.2 / NA	90	A60S (GCA→TCA)	ydgH	DUF1471 domain‑containing protein YdgH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (45/45);  total (45/45)

CTGCTATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCGCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTAA  >  NC_000913/1678467‑1678740                                                                                                                                          |                                                                                                                                         cTGCTATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCa                                                                                                                                        <  1:572648/140‑1 (MQ=255) cTGCTATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCa                                                                                                                                        <  2:196902/140‑1 (MQ=255)   gCTATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGa                                                                                                                                      <  1:372282/140‑1 (MQ=255)       tGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCa                                                                                                                                        <  1:1160828/134‑1 (MQ=255)       tGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCa                                                                                                                                        >  2:1160828/1‑134 (MQ=255)       tGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATaaa                                                                                                                                  >  1:511689/1‑140 (MQ=255)        ggCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAaag                                                                                                                                 >  2:1087416/1‑140 (MQ=255)         gCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAaaga                                                                                                                                >  2:1332582/1‑140 (MQ=255)            ttCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAgg                                                                                                                             >  2:793525/1‑140 (MQ=255)            ttCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAgg                                                                                                                             >  2:1661611/1‑140 (MQ=255)                  gTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgc                                                                                                                       <  2:1180971/140‑1 (MQ=255)                   ttAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                      <  2:1582714/140‑1 (MQ=255)                   ttAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                      <  1:1714719/140‑1 (MQ=255)                   ttAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                      <  1:793525/140‑1 (MQ=255)                   ttAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                      >  1:1153710/1‑140 (MQ=255)                         cagcaACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCttta                                                                                                                >  2:365532/1‑139 (MQ=255)                          agcaACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCttttt                                                                                                               <  2:297665/140‑1 (MQ=255)                            caACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTAt                                                                                                             >  2:637131/1‑140 (MQ=255)                             gcaacaaCTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTAtg                                                                                                            <  2:779600/135‑1 (MQ=255)                                    cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                     <  1:570607/140‑1 (MQ=255)                                    cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                     >  2:986360/1‑140 (MQ=255)                                    cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                     <  1:1661611/140‑1 (MQ=255)                                    cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                     <  1:1332582/140‑1 (MQ=255)                                      gACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGaca                                                                                                   >  1:1397496/1‑140 (MQ=255)                                         acCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACtt                                                                                                <  1:1087416/140‑1 (MQ=255)                                           cGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCt                                                                                              <  1:986360/140‑1 (MQ=255)                                            ggAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTg                                                                                             >  1:1174649/1‑140 (MQ=255)                                                cAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGAttt                                                                                         >  1:1581252/1‑140 (MQ=255)                                                    cGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGt                                                                                     <  2:1174649/140‑1 (MQ=255)                                                        aGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACa                                                                                 <  1:533033/140‑1 (MQ=255)                                                          cGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGc                                                                               <  1:1777193/140‑1 (MQ=255)                                                          cGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGc                                                                               >  2:360622/1‑140 (MQ=255)                                                             ttAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAAc                                                                                  >  1:194117/1‑134 (MQ=255)                                                             ttAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAAc                                                                                  <  2:194117/134‑1 (MQ=255)                                                             ttAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGt                                                                            <  1:355064/140‑1 (MQ=255)                                                               aaaCCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTaa                                                                          <  1:1678741/140‑1 (MQ=255)                                                               aaaCCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTaa                                                                          <  1:360622/140‑1 (MQ=255)                                                                 aCCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACt                                                                        >  2:266067/1‑140 (MQ=255)                                                                      ttGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGTGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCgt                                                                   >  2:743334/1‑140 (MQ=255)                                                                      ttGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCgt                                                                   >  2:1583854/1‑140 (MQ=255)                                                                      ttGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCgt                                                                   >  1:671387/1‑140 (MQ=255)                                                                      ttGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCgt                                                                   >  1:1798166/1‑140 (MQ=255)                                                                       tGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCgtg                                                                  >  1:543617/1‑140 (MQ=255)                                                                            gtgtAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTc                                                             >  1:261248/1‑140 (MQ=255)                                                                             tgtAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCg                                                            >  1:182760/1‑140 (MQ=255)                                                                             tgtAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCg                                                            <  2:1153710/140‑1 (MQ=255)                                                                              gtAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTATCTCGTCGCTCAGAT‑AAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCt                                                          <  2:194145/140‑1 (MQ=255)                                                                               tAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCt                                                          <  1:266067/140‑1 (MQ=255)                                                                               tAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTATATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCt                                                          <  1:365532/140‑1 (MQ=255)                                                                                  tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                       <  1:1460182/140‑1 (MQ=255)                                                                                  tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                       <  1:1415374/140‑1 (MQ=255)                                                                                  tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                       <  2:1607546/140‑1 (MQ=255)                                                                                  tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                       <  1:420496/140‑1 (MQ=255)                                                                                   ggTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCTGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAcc                                                      <  1:637131/140‑1 (MQ=255)                                                                                       aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCta                                                  >  2:609486/1‑140 (MQ=255)                                                                                        ccGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCtat                                                 <  1:605181/140‑1 (MQ=255)                                                                                         cGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                       >  2:1583901/1‑133 (MQ=255)                                                                                         cGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                       <  1:1583901/133‑1 (MQ=255)                                                                                         cGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCtata                                                >  2:662042/1‑140 (MQ=255)                                                                                           gTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATaaa                                              >  2:1389902/1‑140 (MQ=255)                                                                                            tCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAg                                             >  2:1813602/1‑140 (MQ=255)                                                                                            tCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTAGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAg                                             >  1:778058/1‑140 (MQ=255)                                                                                             cGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGc                                            <  2:1039464/140‑1 (MQ=255)                                                                                              gTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGcc                                           >  1:1142512/1‑140 (MQ=255)                                                                                              gTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGcc                                           >  1:1781546/1‑140 (MQ=255)                                                                                                tttAATGCTATTGGCGAAGTGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGa                                         <  1:743334/140‑1 (MQ=255)                                                                                                      gCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGa                                   <  2:1575280/140‑1 (MQ=255)                                                                                                      gCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGa                                   >  2:205825/1‑140 (MQ=255)                                                                                                         aTTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaa                                >  2:1326296/1‑140 (MQ=255)                                                                                                          ttGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaaa                               >  2:1064835/1‑140 (MQ=255)                                                                                                          ttGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaaa                               >  2:420968/1‑140 (MQ=255)                                                                                                           tGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAg                              <  1:1813602/140‑1 (MQ=255)                                                                                                             gCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCa                            <  2:1798166/140‑1 (MQ=255)                                                                                                               gAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCaga                          >  1:624729/1‑140 (MQ=255)                                                                                                                 aGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGcttcat                        <  1:1326296/140‑6 (MQ=255)                                                                                                                 aGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCagaag                        <  2:1375173/140‑1 (MQ=255)                                                                                                                 aGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCagaag                        >  2:48444/1‑140 (MQ=255)                                                                                                                      tGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACa                   >  2:1362865/1‑140 (MQ=255)                                                                                                                          aGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTa               <  2:917863/140‑1 (MQ=255)                                                                                                                           gCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTaa              >  2:878940/1‑140 (MQ=255)                                                                                                                             cGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATc            >  2:1012847/1‑140 (MQ=255)                                                                                                                             cGTTTCTCGTCGCTCAGATAAAGAAGGGGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGGGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATc            >  1:1610650/1‑140 (MQ=255)                                                                                                                             cGTTTCCCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGATAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATc            <  1:1583854/140‑1 (MQ=255)                                                                                                                              gTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCa                            <  1:452953/123‑1 (MQ=255)                                                                                                                              gTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCa                            >  2:452953/1‑123 (MQ=255)                                                                                                                               tttCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgc          >  1:1289149/1‑140 (MQ=255)                                                                                                                               tttCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgc          <  2:1781546/140‑1 (MQ=255)                                                                                                                                ttCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgcg         >  1:440438/1‑140 (MQ=255)                                                                                                                                  ctcGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTa       <  2:671387/140‑1 (MQ=255)                                                                                                                                      tcgCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTa   >  1:1597717/1‑140 (MQ=255)                                                                                                                                       cgctCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTaa  <  1:609486/140‑1 (MQ=255)                                                                                                                                       cgctCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTaa  <  2:1088375/140‑1 (MQ=255)                                                                                                                                       cgctCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGA‑TTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgc          <  1:1143213/131‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         CTGCTATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCGCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTAA  >  NC_000913/1678467‑1678740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑