Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,996,284	G→T	R183R (CGC→CGA)	sdiA ←	DNA‑binding transcriptional dual regulator SdiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,996,284	0	G	T	100.0%	251.7 / NA	71	R183R (CGC→CGA)	sdiA	DNA‑binding transcriptional dual regulator SdiA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (31/40);  total (31/40)

GGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCG  >  NC_000913/1996148‑1996416                                                                                                                                         |                                                                                                                                     gggTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCtcgc                                                                                                                                   >  2:605403/1‑140 (MQ=255)     cTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGc                                                                                                                               <  2:1381413/140‑1 (MQ=255)      ttATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCt                                                                                                                                  >  1:951331/1‑136 (MQ=255)      ttATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCt                                                                                                                                  <  2:951331/136‑1 (MQ=255)        aTTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCTGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGa                                                                                                                            >  2:971920/1‑140 (MQ=255)         tttGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCCGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGaa                                                                                                                           >  2:1439793/1‑140 (MQ=255)            ggTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAAtt                                                                                                                        <  2:1777747/140‑1 (MQ=255)            ggTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAAtt                                                                                                                        >  2:388917/1‑140 (MQ=255)             gTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTc                                                                                                                       >  2:466842/1‑140 (MQ=255)              tGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGc                                                                                                                               <  2:304336/131‑1 (MQ=255)              tGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGc                                                                                                                               >  1:304336/1‑131 (MQ=255)              tGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCa                                                                                                                      >  2:920016/1‑140 (MQ=255)              tGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCa                                                                                                                      >  2:1677068/1‑140 (MQ=255)              tGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCa                                                                                                                      >  2:1410003/1‑140 (MQ=255)                 aTTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCACAATTTCTTTTTCTCGCTTGCTGAAATTCAtct                                                                                                                   <  1:37984/140‑1 (MQ=255)                      tttttttCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGc                                                                                                              <  1:60898/140‑1 (MQ=255)                        tttttCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGt                                                                                                            >  2:884324/1‑140 (MQ=255)                           ttCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGtcat                                                                                                         <  1:361823/140‑1 (MQ=255)                            tCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGtcatc                                                                                                        <  1:971920/140‑1 (MQ=255)                            tCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGtcatc                                                                                                        <  1:884324/140‑1 (MQ=255)                                      tttCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTtcat                                                                                              >  2:499808/1‑140 (MQ=255)                                         cTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTcatcat                                                                                           <  2:1532918/140‑1 (MQ=255)                                          tggtggAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCAtt                                                                                          >  2:1335006/1‑140 (MQ=255)                                             tggAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTaa                                                                                       >  1:59857/1‑140 (MQ=255)                                              ggAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTaaa                                                                                      <  1:388917/140‑1 (MQ=255)                                              ggAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTaaa                                                                                      >  2:1314669/1‑140 (MQ=255)                                               gAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAAc                                                                                     >  1:1222599/1‑140 (MQ=255)                                                      aCCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTtcat                                                                                              <  1:263211/124‑1 (MQ=255)                                                      aCCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTtcat                                                                                              >  2:263211/1‑124 (MQ=255)                                                      aCCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCag                                                                              >  2:1442676/1‑140 (MQ=255)                                                            ttCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTaaa                                                                                      <  1:610303/126‑1 (MQ=255)                                                            ttCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTaaa                                                                                      >  2:610303/1‑126 (MQ=255)                                                            ttCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCAt                                                                        >  1:1290674/1‑140 (MQ=255)                                                            ttCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCAt                                                                        <  1:143403/140‑1 (MQ=255)                                                                 agagATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGAc                                                                   <  2:1425901/140‑1 (MQ=255)                                                                      ttGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTcg                                                              <  2:251123/140‑1 (MQ=255)                                                                      ttGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTcg                                                              <  1:475060/140‑1 (MQ=255)                                                                       tGACAAAATCATCGCTCTCTCTGCTGATGTTTTCCTTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTcgc                                                             <  1:409630/140‑1 (MQ=255)                                                                       tGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTcgc                                                             <  1:605403/140‑1 (MQ=255)                                                                       tGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTcgc                                                             <  1:13480/140‑1 (MQ=255)                                                                       tGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTcgc                                                             <  2:281476/140‑1 (MQ=255)                                                                       tGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTcgc                                                             <  1:1259117/140‑1 (MQ=255)                                                                       tGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTcgc                                                             <  1:1425257/140‑1 (MQ=255)                                                                       tGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTcgc                                                             <  2:1243635/140‑1 (MQ=255)                                                                       tGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTcgc                                                             <  1:850122/140‑1 (MQ=255)                                                                       tGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTcgc                                                             <  1:1314669/140‑1 (MQ=255)                                                                        gACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTcgcg                                                            >  2:1219060/1‑140 (MQ=255)                                                                         aCAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCgcgc                                                           <  1:499808/140‑1 (MQ=255)                                                                         aCAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCgcgc                                                           >  2:827745/1‑140 (MQ=255)                                                                               tcatcGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGt                                                     <  1:827745/140‑1 (MQ=255)                                                                                 atcGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTaa                                                   <  1:1335006/140‑1 (MQ=255)                                                                                      tATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCa                                              <  1:920016/140‑1 (MQ=255)                                                                                             gctgATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTaa                                       >  2:1313030/1‑140 (MQ=255)                                                                                                gATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTg                                    >  1:1582982/1‑140 (MQ=255)                                                                                                gATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTg                                    <  2:869161/140‑1 (MQ=255)                                                                                                gATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTg                                    >  2:1509062/1‑140 (MQ=255)                                                                                                gATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTg                                    <  2:1226712/140‑1 (MQ=255)                                                                                                     tttCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGtt                               <  1:466842/140‑1 (MQ=255)                                                                                                      ttCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTtc                              <  2:794591/140‑1 (MQ=255)                                                                                                            tCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTtc                              <  1:969384/134‑1 (MQ=255)                                                                                                            tCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTtc                              >  2:969384/1‑134 (MQ=255)                                                                                                            tCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACt                        <  1:1313030/140‑1 (MQ=255)                                                                                                            tCCGCCGTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACt                        >  2:614257/1‑140 (MQ=255)                                                                                                                ccgTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCTCTAAGa                    <  1:1439793/140‑1 (MQ=255)                                                                                                                ccgTCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGa                    >  2:114376/1‑140 (MQ=255)                                                                                                                   tCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAg                     <  2:1168573/136‑1 (MQ=255)                                                                                                                   tCCACCTCAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAg                     >  1:1168573/1‑136 (MQ=255)                                                                                                                   tCCACCTCAGAATTTCTTTTCCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATg                 >  2:1681947/1‑140 (MQ=255)                                                                                                                          cAGAATTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTAttt          >  1:200221/1‑140 (MQ=255)                                                                                                                              aTTTCTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgcg      <  2:200221/140‑1 (MQ=255)                                                                                                                                  cTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgcgcgcg  >  2:1755267/1‑140 (MQ=255)                                                                                                                                  cTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgcgcgcg  >  1:251320/1‑140 (MQ=255)                                                                                                                                  cTTTTTCTCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTcgcgcgcg  <  1:1219060/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     GGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCG  >  NC_000913/1996148‑1996416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑