| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,568,504 | 0 | T | G | 35.3% | 71.0 / 11.6 | 52 | T6P (ACC→CCC) | mdtM | multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter |
| Reads supporting (aligned to +/- strand): ref base T (8/25); new base G (18/0); total (27/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.32e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAAT > NC_000913/4568366‑4568639 | cagGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑a > 1:584256/1‑140 (MQ=255) gggCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGG‑aaaa > 1:928285/1‑140 (MQ=255) aGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACg < 1:701963/140‑1 (MQ=255) aCTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTg < 2:928285/140‑1 (MQ=255) aCTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGG‑AAAAAAACGTg > 2:872690/1‑140 (MQ=255) cATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGCCAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGC‑GGAAAAACGtcgggga > 2:751275/1‑134 (MQ=255) cATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATa > 1:780692/1‑140 (MQ=255) cATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAAAGTGGCAAaaaaaac > 1:877291/1‑140 (MQ=255) aTTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATaacaaaca < 1:751275/140‑1 (MQ=255) tAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACg > 1:291122/1‑124 (MQ=255) tAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACg < 2:291122/124‑1 (MQ=255) tACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAAAGTGGCATAACAAACAACTCCTTGTCCg > 1:42014/1‑140 (MQ=255) ccACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGga < 1:896846/140‑1 (MQ=255) cacaTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGgag < 2:627921/140‑1 (MQ=255) aTGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGG‑AAAAAAAAGAGGCATAACAAAAAACACCTTTTCCGGAGAAacccc > 2:215666/1‑139 (MQ=255) aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGG‑AAAAAAACGTGGCATAACAAAAAACTCCTTGTCCGGGGAACCCCAAC‑g > 2:91388/1‑140 (MQ=255) aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGG‑AAAAAAAAGTGGCATAAAAAAAAACACCCTTTCCGGAGAACCCCAAC‑g > 2:6611/1‑140 (MQ=255) aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGG‑AAAAAAAAATGGGATAAAAAAAAACTCCTTGTCCCGAGAAACCCAAC‑g > 2:838413/1‑140 (MQ=255) aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGCG‑AAAAAAACGAGGCCTAAAAAAAAACTCCTTCTCCGGAGGACCCCAAC‑g > 1:72754/1‑140 (MQ=255) cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGG‑AAAAAAAAATGGCATAAAAAACAAATCCTTGTCCGGAGAAACCCCAC‑ggg > 2:314442/1‑140 (MQ=255) ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGG‑AAAAAAAAGAGGCATAAAAAAAAAATCCCTGTCCGGAGAACCCCAAC‑gggg > 2:853742/1‑140 (MQ=255) ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGG‑AAAAAAAAGAGGCATAAAAAAAAAAACCTTGTCCGGAGAACCCCAAC‑gggg > 2:174778/1‑140 (MQ=255) ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGG‑AAAAAAAACTGGCATAACAAAAAACTCCCTGTCCCGAGAAAACCAAC‑aggg > 2:258350/1‑140 (MQ=255) tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACg < 2:780692/140‑1 (MQ=255) tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACg < 1:717769/140‑1 (MQ=255) gATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGG‑AAAAAAACAAGGCATAAAAAAAAAATCCTTGTCCCGAGAAACCCCAC‑GGGGGAAAAGaa > 2:579274/1‑140 (MQ=255) aTCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGG‑AAAAAAAAGTGGGATAAAAAAAAAATCCTTGTCCGGAGAACCCCAAC‑GGGGGAAAAGAAAATCaa > 1:975327/1‑140 (MQ=255) tCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAAt < 2:444755/140‑1 (MQ=255) tCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGG‑AAAAAAAAGTGGCATAAAAAAAAAATCCTTTTCCGGAGAAACCCAAA‑AGGGGAAAAGAAAATCaaa > 2:622672/1‑139 (MQ=255) gTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATAcc < 2:891746/140‑1 (MQ=255) gTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATAcc < 1:622672/140‑1 (MQ=255) tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAAAGTGGCATAACAAAAAACTCCTTGTCCCGAGAAACCCCAC‑GGGGGAAAAGAAAATCAAAAccc > 2:354816/1‑139 (MQ=255) tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAAACAGGAACAACAAAAAACCCCTTGTCCCGAGCACCCCAAA‑GGGGCAAAAGAAAAACAATAcac > 2:951202/1‑138 (MQ=255) tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGG‑AAAAAAAAGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATAccc > 1:283071/1‑139 (MQ=255) tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGG‑AAAAAAAAGTGGCATAAAAAAAAACTCCTTTTCCGGAGAACCCCAAC‑GGGGGAAACGAAAATCAATAccc > 1:441563/1‑139 (MQ=255) tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGAG‑AAAAAAAAGGGGCCTAACAAACAACCCCTTGTCCCGAGGAACCCCAC‑GGGGGAAAAGAAAATAAATAccc > 2:391614/1‑139 (MQ=255) cGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATACCGt < 1:215666/140‑1 (MQ=255) cAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGG‑AAAAAAAAGGGGCATAACAAACAACACCTTTTCCGGAGAACCCCCAC‑CGGGGAAAAGAAAATCAAAACCGTCgg > 2:666973/1‑140 (MQ=255) aTACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATGCCGTCGGTaa < 1:895626/140‑1 (MQ=255) cGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAAAGGGGCATAACAAAAAACTCCCTGTCCGGAGAACCCCAAC‑GGGGGAAAAGAAATTCAATACCGTCGGGAATGa > 2:699202/1‑140 (MQ=255) agcaAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAACGT‑GCATAACAAAAAAACCCTTGTCCGGAGAAACCCCAC‑CGGGAAAAAGAAAATAAATACCCGCGGTAATgcaga > 2:895626/1‑136 (MQ=255) caAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATACCGTCGGTAATGACGaaa < 1:603049/140‑1 (MQ=255) caAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGG‑AAAAAAAAAAGGGATAACAAACAAAACCTTGTCCCGAGAACCCCAAC‑AGGGGAAACGAAAATCAATAACGTCCGGAATGACCaaa > 2:185925/1‑140 (MQ=255) tCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAg < 1:391614/140‑1 (MQ=255) aTACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGt < 2:283321/140‑1 (MQ=255) aTACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGt < 1:699202/140‑1 (MQ=255) aaTCAACGCCATCGTGAAAAACATCGTGGCGGCATGGCGGGG‑AAAAAAACGTGTCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GTGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAg < 2:896541/140‑1 (MQ=255) aaTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAg < 1:174778/140‑1 (MQ=255) aTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGA‑AAAAAAAAGCGGCATAAAAAACAACCCCTTGTCCGGAGAACCCCAAC‑GGGGGAAACGAAAATCAATAACGTCGGGAATGACGAAAGGCAGGGGAGaaag > 2:786237/1‑138 (MQ=255) tCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAAAGTGGCATTACAAAAAACTCCTTTTCCGGAGAACCCCCAC‑GGGGGAAAAGAAATTCAATACCGTCGGGAATGATGAAAAACAGGGGGGAAggg > 2:204440/1‑140 (MQ=255) gCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCaatg < 1:258350/140‑3 (MQ=255) tCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGAc < 1:91388/140‑1 (MQ=255) gAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCa < 2:152760/140‑1 (MQ=255) aGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTAc < 2:441563/140‑1 (MQ=255) gCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACt < 1:184877/140‑1 (MQ=255) gCGTGGCGGCATGGCGGGG‑AAAAAAAAGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCCAC‑GGGGGAAAAGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGGGAGAAGGGCAACGAGACTGCAAATGTACt > 1:637346/1‑140 (MQ=255) ggcggcATGGCGGGT‑AAAAAAAAGTGGCATAAAAAAAAACACCCTGTCCGGAGAACCCCAACGGGGGAAAACAAAATT‑AAAACCCGCGGTAATGACGAAAGGCAGGGGAGAAGGGCAAAGAGACTGCCAAAGTACTGAAt > 2:837204/1‑140 (MQ=255) aTGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATg < 1:69044/140‑1 (MQ=255) aTGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATg < 2:975327/140‑1 (MQ=255) cGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATACCGACGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAAt < 1:853742/140‑1 (MQ=255) cGGGT‑AAAAAAAAGTGGGATAACAAACAACTCCTTGTCCGGGGGACCCCCAC‑GGGGGAAAAGAAATTCAATACCGTCGGGAATGACGAAAAGCAGGGGAGAAGGGGAACGAGACTGCAAATGTAATGAATTTTATGTaaa > 2:704511/1‑139 (MQ=255) | CAGGGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGT‑AAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAGAACCCCAAC‑GGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAAT > NC_000913/4568366‑4568639 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |