Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	33,813	G→T	P999P (CCG→CCT)	carB →	carbamoyl phosphate synthetase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	33,813	0	G	T	100.0%	156.7 / NA	47	P999P (CCG→CCT)	carB	carbamoyl phosphate synthetase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/23);  total (24/23)

GTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCA  >  NC_000913/33685‑33943                                                                                                                                 |                                                                                                                                   gtggtgGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGa                                                                                                                         >  1:59525/1‑140 (MQ=255)  tggtggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGAc                                                                                                                        <  1:968068/140‑1 (MQ=255)   ggtggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGAcc                                                                                                                       >  1:713279/1‑140 (MQ=255)   ggtggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGAcc                                                                                                                       <  1:644723/140‑1 (MQ=255)       gACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTAt                                                                                                                   <  2:784874/140‑1 (MQ=255)           tGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGGAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAg                                                                                                               >  2:615022/1‑140 (MQ=255)             gcggcAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGaa                                                                                                             >  1:209853/1‑140 (MQ=255)             gcggcAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGaa                                                                                                             >  1:283500/1‑140 (MQ=255)              cggcAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAAt                                                                                                            >  2:970347/1‑140 (MQ=255)               ggcAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATg                                                                                                           <  2:772863/140‑1 (MQ=255)               ggcAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATg                                                                                                           <  2:460614/140‑1 (MQ=255)                gcAAAACGGCTGAAACAGGATTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATTAAGGCCGTCCTCACATTCAGGATCGTATCAAGAATgg                                                                                                          <  2:486910/140‑1 (MQ=255)                    aaCTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGaa                                                                                                      <  2:58395/140‑1 (MQ=255)                                 gggCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTAcatca                                                                                         <  2:713279/140‑1 (MQ=255)                                  ggCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTAcatcat                                                                                        <  1:866250/140‑1 (MQ=255)                                    cTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACAtcatca                                                                                      >  1:747601/1‑140 (MQ=255)                                      tCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAac                                                                                    >  2:222706/1‑140 (MQ=255)                                          gCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAAcacca                                                                                <  1:14162/140‑1 (MQ=255)                                            tGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACaccacc                                                                              >  1:732988/1‑140 (MQ=255)                                             ggATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCt                                                                             <  1:222706/140‑1 (MQ=255)                                                         cggcacggcGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTg                                                                 <  2:283500/140‑1 (MQ=255)                                                          ggcacggcGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGc                                                                <  2:490356/140‑1 (MQ=255)                                                                    ttGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGAc                                                      >  2:46374/1‑140 (MQ=255)                                                                      gtgCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTc                                                    >  1:442792/1‑140 (MQ=255)                                                                      gtgCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTc                                                    <  1:481671/140‑1 (MQ=255)                                                                           gggCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCcgcg                                               <  1:819185/140‑1 (MQ=255)                                                                            ggCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGt                                              >  1:12391/1‑140 (MQ=255)                                                                               gAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGAt                                           <  2:747601/140‑1 (MQ=255)                                                                                 aGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATtc                                         >  1:617104/1‑140 (MQ=255)                                                                                      gTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGc                                    >  1:763358/1‑140 (MQ=255)                                                                                      gTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGc                                    >  2:520022/1‑140 (MQ=255)                                                                                          cAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTg                                <  1:520022/140‑1 (MQ=255)                                                                                           aaCCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTgc                               >  1:461927/1‑140 (MQ=255)                                                                                           aaCCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTgc                               >  1:236104/1‑140 (MQ=255)                                                                                            aCCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTgcg                              >  1:474685/1‑140 (MQ=255)                                                                                            aCCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACC‑ACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTgcgc                             >  2:287352/1‑140 (MQ=255)                                                                                              ccGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCt                            <  2:617104/140‑1 (MQ=255)                                                                                              ccGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCt                            <  2:12391/140‑1 (MQ=255)                                                                                                 cgTCTGGTAAACAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCa                         >  2:893408/1‑140 (MQ=255)                                                                                                           aCAAGGTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTg               <  1:970347/140‑1 (MQ=255)                                                                                                               ggTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCAGCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCAtt           >  2:724267/1‑140 (MQ=255)                                                                                                                gTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTa          >  1:503959/1‑140 (MQ=255)                                                                                                                gTGCATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTa          >  1:159548/1‑140 (MQ=255)                                                                                                                   cATGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGa       >  2:447129/1‑140 (MQ=255)                                                                                                                     tGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGaca     <  2:503959/140‑1 (MQ=255)                                                                                                                     tGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGaca     <  2:732988/140‑1 (MQ=255)                                                                                                                     tGAAGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGaca     <  2:461927/140‑1 (MQ=255)                                                                                                                        aGGCCGTCCTCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGAcacca  >  2:54697/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                   GTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATGAAGGCCGTCCGCACATTCAGGACCGTATCAAGAATGGCGAATATACCTACATCATCAACACCACCTCAGGCCGTCGTGCGATTGAAGACTCCCGCGTGATTCGTCGCAGTGCGCTGCAATATAAAGTGCATTACGACACCA  >  NC_000913/33685‑33943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑