Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,045,847	G→T	intergenic (‑44/+2)	gfcE ← / ← gfcD	putative exopolysaccharide export lipoprotein GfcE/putative lipoprotein GfcD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,045,847	0	G	T	100.0%	168.2 / NA	49	intergenic (‑44/+2)	gfcE/gfcD	putative exopolysaccharide export lipoprotein GfcE/putative lipoprotein GfcD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/20);  total (29/20)

GTCGATAAATTCTGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCAGGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCGCGCGGTTGCGGTTAGGTCCAATGGTC  >  NC_000913/1045712‑1045981                                                                                                                                        |                                                                                                                                       gTCGATAAATTCTGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTa                                                                                                                                    >  1:693757/1‑140 (MQ=255)      tAAATTCTGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTc                                                                                                                               <  1:125625/140‑1 (MQ=255)        aaTTCTGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCa                                                                                                                             <  1:966743/140‑1 (MQ=255)           tCTGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGt                                                                                                                                      >  2:215350/1‑128 (MQ=255)              gACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGt                                                                                                                       >  2:78342/1‑140 (MQ=255)              gACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGt                                                                                                                       >  1:421211/1‑140 (MQ=255)                    gAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTtct                                                                                                                 >  1:24119/1‑140 (MQ=255)                            gTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGc                                                                                                         <  1:777317/140‑1 (MQ=255)                               cATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGt                                                                                                      >  2:726707/1‑140 (MQ=255)                                 tGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCa                                                                                                    <  2:24119/140‑1 (MQ=255)                                     gTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTgg                                                                                                >  2:816869/1‑140 (MQ=255)                                     gTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTgg                                                                                                >  2:684139/1‑140 (MQ=255)                                     gTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTgg                                                                                                >  1:722751/1‑140 (MQ=255)                                      tCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGa                                                                                               <  1:559174/140‑1 (MQ=255)                                      tCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGa                                                                                               <  1:87749/140‑1 (MQ=255)                                      tCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGa                                                                                               <  1:78342/140‑1 (MQ=255)                                      tCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGa                                                                                               <  1:363205/140‑1 (MQ=255)                                           gACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATagag                                                                                          >  2:144449/1‑140 (MQ=255)                                                aaCAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGt                                                                                     >  2:610076/1‑140 (MQ=255)                                                aaCAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGt                                                                                     >  2:351564/1‑140 (MQ=255)                                                aaCAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGt                                                                                     >  2:35490/1‑140 (MQ=255)                                                aaCAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGt                                                                                     <  2:421211/140‑1 (MQ=255)                                                         tGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTg                                                                            >  1:397598/1‑140 (MQ=255)                                                          gTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGa                                                                           >  1:555537/1‑140 (MQ=255)                                                               tACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCG‑CATTGGATAGAGCTGGTATTTGCGTGACAGCAt                                                                     <  1:803143/140‑1 (MQ=255)                                                                aCCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCAt                                                                     <  1:726707/140‑1 (MQ=255)                                                                aCCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCAt                                                                     <  1:254755/140‑1 (MQ=255)                                                                    aaaaCTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGt                                                                 >  1:701318/1‑140 (MQ=255)                                                                           aaaaaTGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGTATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCAt                                                          >  2:732155/1‑140 (MQ=255)                                                                             aaaTGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCa                                                        <  1:351564/140‑1 (MQ=255)                                                                                       tttCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATg                                              <  1:332742/140‑1 (MQ=255)                                                                                                tttATATTTATCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACg                                     <  1:144449/140‑1 (MQ=255)                                                                                                         aTCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACcg                            >  1:822869/1‑140 (MQ=255)                                                                                                         aTCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACcg                            >  2:894469/1‑140 (MQ=255)                                                                                                         aTCAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGCGTCCACGAAACCACcg                            >  2:973132/1‑140 (MQ=255)                                                                                                           cAGTCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGCGTCCACGAAACCACcgcg                          <  1:973132/140‑1 (MQ=255)                                                                                                              tCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCGCgcgg                       >  1:900631/1‑140 (MQ=255)                                                                                                              tCAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCGCgcgg                       >  1:838472/1‑140 (MQ=255)                                                                                                               cAATGTAATGTTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCGCgcggt                      <  2:693757/140‑1 (MQ=255)                                                                                                                 atgtaatgtTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCGCgcggttg                    <  2:397598/140‑1 (MQ=255)                                                                                                                     aatgtTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCGCgcggttgcggt                <  2:701318/140‑1 (MQ=255)                                                                                                                       tgtTATGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCGCGCGGTTGCGGTTa              >  1:20698/1‑140 (MQ=255)                                                                                                                           aTGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGTATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCGCGCGGTTGCGGTTAGGTc          <  2:185682/140‑1 (MQ=255)                                                                                                                            tGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCGCGCGGTTGCGGTTAGGTcc         >  2:619389/1‑140 (MQ=255)                                                                                                                            tGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCGCGCGGTTGCGGTTAGGTcc         >  2:666101/1‑140 (MQ=255)                                                                                                                            tGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCGCGCGGTTGCGGTTAGGTcc         >  2:811460/1‑140 (MQ=255)                                                                                                                            tGAATTACGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCGCGCGGTTGCGGTTAGGTcc         >  2:824061/1‑140 (MQ=255)                                                                                                                                   cGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCGCGCGGTTGCGGTTAGGTCCAATGGtc  >  2:826479/1‑140 (MQ=255)                                                                                                                                   cGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCGCGCGGTTGCGGTTAGGTCCAATGGtc  >  1:692844/1‑140 (MQ=255)                                                                                                                                   cGTCATGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCGCGCGGTTGCGGTTAGGTCCAATGGtc  >  2:901207/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       GTCGATAAATTCTGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCAGGTTATTGTCCAACCGGTACTTCTCGCTCTGCCGTCATTGGATAGAGCTGGTATTTGCGTGACAGCATTTGTCCACCATCACGCGTCAATGGTGTCCACGAAACCACCGCGCGGTTGCGGTTAGGTCCAATGGTC  >  NC_000913/1045712‑1045981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑