Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,218,872	A→G	intergenic (+21/‑111)	pdeG → / → ymgF	putative c‑di‑GMP phosphodiesterase PdeG/inner membrane protein that interacts with cell division proteins

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,218,872	0	A	G	100.0%	192.5 / NA	52	intergenic (+21/‑111)	pdeG/ymgF	putative c‑di‑GMP phosphodiesterase PdeG/inner membrane protein that interacts with cell division proteins
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/31);  total (21/31)

TGAACCAAAATTATATCACATTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATAGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTG  >  NC_000913/1218736‑1219003                                                                                                                                         |                                                                                                                                    tGAACCAAAATTATATCACATTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAATGTTGTGGTTCAGTGAAAATTGATCAGTAAGGCCATGGTg                                                                                                                                  <  2:686339/140‑1 (MQ=255) tGAACCAAAATTATATCACATTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTg                                                                                                                                  <  1:859969/140‑1 (MQ=255) tGAACCAAAATTATATCACATTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTg                                                                                                                                  <  1:366834/140‑1 (MQ=255) tGAACCAAAATTATATCACATTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTg                                                                                                                                  <  2:561324/140‑1 (MQ=255) tGAACCAAAATTATATCACATTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTg                                                                                                                                  <  1:301861/140‑1 (MQ=255)    aCCAAAATTATATCACATTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCgg                                                                                                                               <  1:915167/140‑1 (MQ=255)     ccAAAATTATATCACATTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGgt                                                                                                                              >  2:811148/1‑140 (MQ=255)      cAAAATTATATCACATTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGgtg                                                                                                                             <  1:696890/140‑1 (MQ=255)        aaaTTATATCACATTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTa                                                                                                                           <  1:952767/140‑1 (MQ=255)         aaTTATATCACATTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTaa                                                                                                                          >  2:716669/1‑140 (MQ=255)              tatCACATTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGGCCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATtata                                                                                                                     <  1:716669/140‑1 (MQ=255)                tCACATTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGa                                                                                                                   <  2:959752/140‑1 (MQ=255)                 cacaTTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTTAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGAc                                                                                                                  <  1:833532/140‑1 (MQ=255)                  acaTTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACa                                                                                                                 <  1:428600/140‑1 (MQ=255)                   caTTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAg                                                                                                                >  1:120892/1‑140 (MQ=255)                   caTTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAg                                                                                                                >  1:454314/1‑140 (MQ=255)                                     attTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCct                                                                                              <  1:952009/140‑1 (MQ=255)                                     attTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCct                                                                                              <  2:120892/140‑1 (MQ=255)                                     attTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCct                                                                                              <  2:577980/140‑1 (MQ=255)                                     attTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCct                                                                                              <  1:117222/140‑1 (MQ=255)                                        tCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCct                                                                                              <  2:872982/137‑1 (MQ=255)                                        tCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCct                                                                                              >  1:872982/1‑137 (MQ=255)                                        tCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCtt                                                                                           >  2:837980/1‑140 (MQ=255)                                        tCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCtt                                                                                           >  1:133804/1‑140 (MQ=255)                                        tCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCtt                                                                                           >  2:347620/1‑140 (MQ=255)                                         cTATAAACCTGTTACATACATCGACTTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTg                                                                                          >  1:60559/1‑140 (MQ=255)                                         cTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCct                                                                                              >  2:407794/1‑136 (MQ=255)                                         cTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCct                                                                                              <  1:407794/136‑1 (MQ=255)                                                              cGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTTATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATtttg                                                                     >  1:869460/1‑140 (MQ=255)                                                              cGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGGGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATtttg                                                                     >  2:705208/1‑140 (MQ=255)                                                                gccTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCGATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTTCTATTGTTCATTATtttgtt                                                                   <  2:815539/139‑1 (MQ=255)                                                                aCCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATtttgtt                                                                   <  2:597813/140‑1 (MQ=255)                                                                aCCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATtttgtt                                                                   <  1:357104/140‑1 (MQ=255)                                                                 ccTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATtttgttt                                                                  >  2:934720/1‑140 (MQ=255)                                                                      tCAAAATTATCCTTTCTAAACCGAAGGAGAAGGTTGTGGTTGAGGGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTTAAGACAGCTAATTGCCACGTTGCCTCTTGTTACTATTGTTCTTTTTTTTGTTTGCttt                                                             >  2:233378/1‑138 (MQ=255)                                                                               tCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTTATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTg                                                    >  2:690428/1‑140 (MQ=255)                                                                                   ttCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAg                                                <  1:262012/140‑1 (MQ=255)                                                                                    tCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGt                                               >  2:110080/1‑140 (MQ=255)                                                                                         aCCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGa                                          <  1:110080/140‑1 (MQ=255)                                                                                         aCCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGa                                          >  1:151713/1‑140 (MQ=255)                                                                                           cGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACa                                        <  1:934720/140‑1 (MQ=255)                                                                                              aggtgaaggtTGTGGTTGAGTGAAAATTTATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACAGGa                                     <  1:690428/140‑1 (MQ=255)                                                                                              aggtgaaggtTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACAGGa                                     <  1:9831/140‑1 (MQ=255)                                                                                               ggtgaaggtTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACAGGAt                                    >  1:413638/1‑140 (MQ=255)                                                                                                  gaaggtTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACAGGATTGc                                 <  2:413638/140‑1 (MQ=255)                                                                                                   aaggtTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACAGGATTg                                  <  1:115773/138‑1 (MQ=255)                                                                                                   aaggtTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACAGGATTg                                  >  2:115773/1‑138 (MQ=255)                                                                                                          tgGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACAGGATTGCCATtagta                         >  1:199563/1‑140 (MQ=255)                                                                                                          tgGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACAGGATTGCCATtagta                         >  2:542773/1‑140 (MQ=255)                                                                                                          tgGTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACAGGATTGCCATtagta                         >  1:555149/1‑140 (MQ=255)                                                                                                            gTTGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGc                       <  2:372535/140‑1 (MQ=255)                                                                                                             ttGAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCa                      <  2:810788/140‑1 (MQ=255)                                                                                                               gAGTGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACCATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATg                    <  2:434480/140‑1 (MQ=255)                                                                                                                  tGAAAATTGATCAGTAAGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAAc                 <  2:869285/140‑1 (MQ=255)                                                                                                                              aGTAAGGGCATTGGGCGGTGGAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACAGGATAGCCATTAGTAGCATGAACAATAGTaataat     <  1:233378/140‑1 (MQ=255)                                                                                                                                 aaGGCCATGGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTg  >  1:190477/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    TGAACCAAAATTATATCACATTTCAGCAGGGTTATTATTTCTATAAACCTGTTACATACATCGACCTGGTCAAAATTATCCTTTCTAAACCGAAGGTGAAGGTTGTGGTTGAGTGAAAATTGATCAGTAAGGCCATAGTGCGGTGTAATTATAGACAGCTAATTAGCTCGTTGCCTCTTGTTACTATTGTTCATTATTTTGTTTGCTATAATTGTTTGAAAGTTTTGACAGGATTGCCATTAGTAGCATGAACAATAGTAATAATCTG  >  NC_000913/1218736‑1219003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑