Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,659,545	G→T	intergenic (‑78/+18)	hscB ← / ← iscA	co‑chaperone for [Fe‑S] cluster biosynthesis/iron‑sulfur cluster insertion protein IscA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,659,545	0	G	T	100.0%	109.5 / NA	34	intergenic (‑78/+18)	hscB/iscA	co‑chaperone for [Fe‑S] cluster biosynthesis/iron‑sulfur cluster insertion protein IscA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/15);  total (19/15)

TGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAAATTGC  >  NC_000913/2659415‑2659680                                                                                                                                   |                                                                                                                                        tGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCgg                                                                                                                                >  1:125972/1‑140 (MQ=255)            ttGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCa                                                                                                                     <  2:612311/140‑1 (MQ=255)              gATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGCCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGTTGGGGTTTGCGGTATGCGCATCaaa                                                                                                                   >  2:705635/1‑140 (MQ=255)               aTAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAAc                                                                                                                  >  1:347563/1‑140 (MQ=255)               aTAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGAAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGGTATCGGTATGCGCATCAAAc                                                                                                                  >  2:380563/1‑140 (MQ=255)                                       ggTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACcaca                                                                                          <  1:496165/140‑1 (MQ=255)                                       ggTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACcaca                                                                                          <  2:360400/140‑1 (MQ=255)                                        gTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCacac                                                                                         >  2:18283/1‑140 (MQ=255)                                         tGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACt                                                                                        >  1:558948/1‑140 (MQ=255)                                          gAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTc                                                                                       >  1:820122/1‑140 (MQ=255)                                            aGTAATCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCAt                                                                                     <  1:449423/140‑1 (MQ=255)                                                 tCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTg                                                                                >  2:634430/1‑140 (MQ=255)                                                   cATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGAc                                                                              >  1:201248/1‑140 (MQ=255)                                                      aaCATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGtt                                                                           >  1:875336/1‑140 (MQ=255)                                                      aaCATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGtt                                                                           <  2:700042/140‑1 (MQ=255)                                                          ttCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCggg                                                                       >  1:314819/1‑140 (MQ=255)                                                          ttCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCggg                                                                       <  1:634430/140‑1 (MQ=255)                                                                gggCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTg                                                                 <  2:761869/140‑1 (MQ=255)                                                                 ggCTGCGATCCGTCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCACGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACCTGGAAGCTTTCGCCGCAACCACACCCATCTTTGACGTTCTGGGTGGTGa                                                                >  2:601163/1‑140 (MQ=255)                                                                 ggCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGa                                                                >  2:78359/1‑140 (MQ=255)                                                                  gCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGaa                                                               >  1:840397/1‑140 (MQ=255)                                                                       gATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTAGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTaa                                                          <  2:664065/140‑1 (MQ=255)                                                                                 gATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGtt                                                <  2:201248/140‑1 (MQ=255)                                                                                       ggCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGcc                                          <  1:843443/140‑1 (MQ=255)                                                                                           aaTCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCcttct                                      >  2:102143/1‑140 (MQ=255)                                                                                            aTCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCcttctt                                     <  2:840397/140‑1 (MQ=255)                                                                                               aGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTa                                  <  2:62033/140‑1 (MQ=255)                                                                                                ggTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTAc                                 >  1:625008/1‑140 (MQ=255)                                                                                                 gTAAAACAAACCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACg                                <  2:347563/140‑1 (MQ=255)                                                                                                     aacaaacCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGt                            >  1:274575/1‑140 (MQ=255)                                                                                                      acaaacCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTc                           <  1:601163/140‑1 (MQ=255)                                                                                                          acCCCACGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGc                       >  1:669055/1‑140 (MQ=255)                                                                                                           ccccACGCGCAGGCAACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCt                      <  2:1220/140‑1 (MQ=255)                                                                                                               aCGCGCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGt                  >  2:194842/1‑140 (MQ=255)                                                                                                                cgcgCAGGCGACCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGGAGCTTTCGCTGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTa                 >  1:773353/1‑140 (MQ=255)                                                                                                                          aCCACGGTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGaa       >  1:106730/1‑140 (MQ=255)                                                                                                                               ggTGTGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAAATTGc  <  1:380563/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                        TGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAAATTGC  >  NC_000913/2659415‑2659680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑