Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,126,933	C→A	L363L (CTG→CTT)	glcD ←	glycolate dehydrogenase, putative FAD‑linked subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,126,933	0	C	A	100.0%	177.2 / NA	52	L363L (CTG→CTT)	glcD	glycolate dehydrogenase, putative FAD‑linked subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/29);  total (23/29)

CGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGT  >  NC_000913/3126799‑3127067                                                                                                                                       |                                                                                                                                       cgGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGgc                                                                                                                                   >  1:154557/1‑140 (MQ=255) cgGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGgc                                                                                                                                   >  2:80435/1‑140 (MQ=255)  gggCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGgcg                                                                                                                                  <  2:783168/140‑1 (MQ=255)  gggCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGgcg                                                                                                                                  <  1:4984/140‑1 (MQ=255)        aTTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGAcgc                                                                                                                            <  2:320521/140‑1 (MQ=255)            tccGGCTTCGTCTGCATCGAAAAGGATTAACGGGTGCATGTTGCAATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGa                                                                                                                        <  2:602977/139‑1 (MQ=255)            aCCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGa                                                                                                                        >  2:585429/1‑140 (MQ=255)            aCCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGa                                                                                                                        <  1:119704/140‑1 (MQ=255)              cGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGa                                                                                                                        >  2:606575/1‑138 (MQ=255)              cGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGa                                                                                                                        <  1:606575/138‑1 (MQ=255)              cGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATg                                                                                                                      >  1:423258/1‑140 (MQ=255)                ggTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGt                                                                                                                    <  2:423258/140‑1 (MQ=255)                 gTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTg                                                                                                                   <  1:80435/140‑1 (MQ=255)                   tCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGcc                                                                                                                 <  2:170012/140‑1 (MQ=255)                     gTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGccat                                                                                                               <  1:420002/140‑1 (MQ=255)                     gTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGccat                                                                                                               <  1:839838/140‑1 (MQ=255)                          cATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATg                                                                                                          <  1:585429/140‑1 (MQ=255)                          cATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATg                                                                                                          <  1:182591/140‑1 (MQ=255)                          cATCGAAAAGGATTAACGGGTGCATGTTACCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATg                                                                                                          <  1:177545/140‑1 (MQ=255)                                    gATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAAt                                                                                                <  1:490715/140‑1 (MQ=255)                                        aaCGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCgg                                                                                            <  1:804338/140‑1 (MQ=255)                                         aCGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCggg                                                                                           >  1:463952/1‑140 (MQ=255)                                         aCGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCggg                                                                                           <  1:549191/140‑1 (MQ=255)                                         aCGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCggg                                                                                           <  1:813757/140‑1 (MQ=255)                                              tGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCgg                                                                                            <  2:967444/134‑1 (MQ=255)                                              tGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCgg                                                                                            >  1:967444/1‑134 (MQ=255)                                              tGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGAt                                                                                      >  2:651148/1‑140 (MQ=255)                                              tGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGAt                                                                                      >  2:44875/1‑140 (MQ=255)                                                       ccATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTAc                                                                             >  2:558581/1‑140 (MQ=255)                                                               ggCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGa                                                                     >  2:63159/1‑140 (MQ=255)                                                                      aaGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCAtt                                                              >  2:379603/1‑140 (MQ=255)                                                                              ggCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGGGATACGTCCTGCCGCCGGGAACGCATTTTTGCGAc                                                      <  1:880314/140‑1 (MQ=255)                                                                              ggCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGAc                                                      <  1:766208/140‑1 (MQ=255)                                                                              ggCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGAc                                                      <  1:320376/140‑1 (MQ=255)                                                                              ggCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGAc                                                      <  1:558581/140‑1 (MQ=255)                                                                              ggCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGAc                                                      >  2:940405/1‑140 (MQ=255)                                                                              ggCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAAGCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGAc                                                      <  2:289561/140‑1 (MQ=255)                                                                                cAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCg                                                    <  1:63159/140‑1 (MQ=255)                                                                                         aaTCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGaaa                                           >  2:844447/1‑140 (MQ=255)                                                                                             atatTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTa                                       <  1:940405/140‑1 (MQ=255)                                                                                              tatTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTAc                                      >  1:392287/1‑140 (MQ=255)                                                                                                ttGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTAcgc                                    >  2:571267/1‑140 (MQ=255)                                                                                                   ctgcGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGct                                 <  1:571267/140‑1 (MQ=255)                                                                                                    tgcGATAAACGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGctc                                >  2:317044/1‑140 (MQ=255)                                                                                                             cGGGCAATGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTc                       <  2:463952/140‑1 (MQ=255)                                                                                                                   aTGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGc                 >  2:564923/1‑140 (MQ=255)                                                                                                                   aTGCCTTCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGc                 >  2:237348/1‑140 (MQ=255)                                                                                                                         tCCAGTACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAgacg           <  1:208488/140‑1 (MQ=255)                                                                                                                              tACGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGt      >  1:625354/1‑140 (MQ=255)                                                                                                                               aCGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTc     >  2:507528/1‑140 (MQ=255)                                                                                                                                cGCCAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCa    >  2:552872/1‑140 (MQ=255)                                                                                                                                  ccAGGAAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGt  >  2:695895/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                       CGGGCAAATTCACCGGGTTCGTTGGCATCGAAAAGGATTAACGGGTGCATGTTGCCATCTCCGGCATGAAAGACGTTGGCAACACGTAAATCATATTGCTGCGATAAACGGGCAATGCCTTCCAGTACGCCAGGCAGGGCGCGACGCGGGATGGTGCCATCCATGCAGTAGTAATCCGGGGAGATACGTCCTACCGCCGGGAACGCATTTTTGCGACCGGCCCAGAAACGTACGCGCTCTGCTTCGTCCTGTGCCAGACGGACGTCAGT  >  NC_000913/3126799‑3127067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑