Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	237,845	G→T	D171Y (GAC→TAC)	yafT →	lipoprotein YafT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	237,845	0	G	T	100.0%	174.5 / NA	51	D171Y (GAC→TAC)	yafT	lipoprotein YafT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/29);  total (22/29)

CAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATGACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGATGTGGCAACAGGTGAGTGGGCTACCTGGTCGCCGGT  >  NC_000913/237709‑237978                                                                                                                                         |                                                                                                                                      caaaGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTAct                                                                                                                                    <  1:840277/140‑1 (MQ=255)  aaaGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTActc                                                                                                                                   >  1:617781/1‑140 (MQ=255)      aTAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTgcg                                                                                                                               <  2:230340/140‑1 (MQ=255)          aaaTGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGG‑TGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGaa                                                                                                                          <  2:839174/140‑1 (MQ=255)                gaACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCaa                                                                                                                     <  2:515906/140‑1 (MQ=255)                    gaggagGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGt                                                                                                                 <  1:245211/140‑1 (MQ=255)                         tGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGt                                                                                                            <  2:918869/140‑1 (MQ=255)                           ttGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTcc                                                                                                          <  1:692094/140‑1 (MQ=255)                           ttGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCACTGAAATCAACAGTATGGTcc                                                                                                          >  1:144483/1‑140 (MQ=255)                                  tGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACtata                                                                                                   >  2:930858/1‑140 (MQ=255)                                       agaATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAAt                                                                                              >  2:973923/1‑140 (MQ=255)                                            atGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACaa                                                                                         >  1:563271/1‑140 (MQ=255)                                              gAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAAc                                                                                       >  2:551127/1‑140 (MQ=255)                                                aCTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTc                                                                                     >  2:525621/1‑140 (MQ=255)                                                  tGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCAc                                                                                   <  1:827113/140‑1 (MQ=255)                                                   ggATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACt                                                                                  >  2:287658/1‑140 (MQ=255)                                                         aGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCt                                                                            >  2:816647/1‑140 (MQ=255)                                                             aCTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTAtct                                                                        <  2:563271/140‑1 (MQ=255)                                                              cTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTAtctc                                                                       >  1:75558/1‑140 (MQ=255)                                                               tGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTcc                                                                      >  2:423066/1‑140 (MQ=255)                                                               tGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTcc                                                                      <  1:973923/140‑1 (MQ=255)                                                                    tttGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTgcg                                                                 <  1:810069/140‑1 (MQ=255)                                                                    tttGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTgcg                                                                 <  1:828705/140‑1 (MQ=255)                                                                    tttGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTgcg                                                                 <  1:92883/140‑1 (MQ=255)                                                                             gCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGt                                                        <  2:701780/140‑1 (MQ=255)                                                                                     aCATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCa                                                <  1:937417/140‑1 (MQ=255)                                                                                     aCATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCa                                                <  2:75558/140‑1 (MQ=255)                                                                                      cATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCACTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCAc                                               <  2:144483/140‑1 (MQ=255)                                                                                        tCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGc                                             >  2:515255/1‑140 (MQ=255)                                                                                          aGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCtg                                           <  2:470996/140‑1 (MQ=255)                                                                                             aaGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCtggtg                                        <  1:567919/140‑1 (MQ=255)                                                                                              aGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCtggtgg                                       >  1:816916/1‑140 (MQ=255)                                                                                                cGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACg                                              <  2:640058/131‑1 (MQ=255)                                                                                                cGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACg                                              >  1:640058/1‑131 (MQ=255)                                                                                                cGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGAt                                     <  1:515255/140‑1 (MQ=255)                                                                                                 gATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGAtg                                    <  2:24701/140‑1 (MQ=255)                                                                                                 gATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGAtg                                    >  2:415219/1‑140 (MQ=255)                                                                                                  attattGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGAtgt                                   <  1:99616/140‑1 (MQ=255)                                                                                                  attattGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGAtgt                                   <  1:299599/140‑1 (MQ=255)                                                                                                      ttGTTTACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGATGTGGCa                               >  2:813300/1‑140 (MQ=255)                                                                                                       tGTATACCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGATGTGGCaa                              <  2:385100/140‑1 (MQ=255)                                                                                                            aCCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGATGTGGCAACAGGt                         <  1:423066/140‑1 (MQ=255)                                                                                                            aCCAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGATGGGGCAACAggg                         >  1:676399/1‑139 (MQ=255)                                                                                                             ccAGGATATGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGATGTGGCAACAGGTg                        <  2:881258/140‑1 (MQ=255)                                                                                                                   tatGTTGCAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAGATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGATGTGGCAACAGGTGAGTggg                  >  1:722426/1‑140 (MQ=255)                                                                                                                          cAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGATGTGGCAACAGGTGAg                      <  2:688075/129‑1 (MQ=255)                                                                                                                          cAGACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGATGTGGCAACAGGTGAg                      >  1:688075/1‑129 (MQ=255)                                                                                                                            gACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGATGTGGCAACAGGTGAGTGGGCTACCTGGt         >  2:433628/1‑140 (MQ=255)                                                                                                                            gACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGATGTGGCAACAGGTGAGTGGGCTACCTGGt         >  2:391101/1‑140 (MQ=255)                                                                                                                            gACAGGAAAATATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGATGTGGCAACAGGTGAGTGGGCTACCTGGt         >  1:282168/1‑140 (MQ=255)                                                                                                                                   aaaTATTACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGATGTGGCAACAGGTGAGTGGGCTACCTGGTCGCCGGt  <  1:930858/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      CAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGACATCAGAAAGCGATTATTGTTTACCAGGATATGTTGCAGACAGGAAAATATGACTCTGCGCTGAAATCAACAGTATGGTCCGACTATAAAAATGACAAACTCACTGACGCTATCTCCCTGCGCTACCTGGTACGTTTCACGCTGGTGGATGTGGCAACAGGTGAGTGGGCTACCTGGTCGCCGGT  >  NC_000913/237709‑237978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑