Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,535,950 A→T V212E (GTG→GAG)  ompR ← DNA‑binding transcriptional dual regulator OmpR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,535,9500AT97.9% 156.3 / ‑3.9 47V212E (GTG→GAG) ompRDNA‑binding transcriptional dual regulator OmpR
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (22/24);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

CAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCG  >  NC_000913/3535818‑3536085
                                                                                                                                    |                                                                                                                                       
cAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATgcg                                                                                                                                  >  2:877468/1‑140 (MQ=255)
     aCGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCa                                                                                                                             <  2:525882/140‑1 (MQ=255)
                    aaCTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCa                                                                                                              <  1:971758/140‑1 (MQ=255)
                          gTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGa                                                                                                        <  2:696284/140‑1 (MQ=255)
                          gTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGa                                                                                                        <  2:772951/140‑1 (MQ=255)
                            ggCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATg                                                                                                      <  2:180042/140‑1 (MQ=255)
                            ggCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATg                                                                                                      >  1:417877/1‑140 (MQ=255)
                               gagaAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAg                                                                                                   <  2:783788/140‑1 (MQ=255)
                                      gcAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCa                                                                                            <  2:792737/140‑1 (MQ=255)
                                             ccTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGa                                                                                     <  2:827169/140‑1 (MQ=255)
                                             ccTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGa                                                                                     <  1:284894/140‑1 (MQ=255)
                                               tCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAat                                                                                   >  2:971246/1‑140 (MQ=255)
                                                    cTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCa                                                                              <  1:918558/140‑1 (MQ=255)
                                                               tCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGc                                                                   >  1:975117/1‑140 (MQ=255)
                                                               tCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGAGc                                                                   >  2:784133/1‑140 (MQ=255)
                                                                 cGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCaa                                                                 <  1:971246/140‑1 (MQ=255)
                                                                   gTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAgg                                                               <  1:12062/140‑1 (MQ=255)
                                                                    tACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGt                                                              <  2:203458/140‑1 (MQ=255)
                                                                              tAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCtt                                                    >  2:302308/1‑140 (MQ=255)
                                                                              tAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCACTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCtt                                                    >  2:52543/1‑140 (MQ=255)
                                                                               aGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTa                                                   <  1:877468/140‑1 (MQ=255)
                                                                                     gaccccagacGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCgg                                             >  1:684988/1‑140 (MQ=255)
                                                                                     gaccccagacGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCgg                                             >  2:201459/1‑140 (MQ=255)
                                                                                                gTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTc                                  <  1:201459/140‑1 (MQ=255)
                                                                                                  cTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCa                                 <  2:99453/139‑1 (MQ=255)
                                                                                                  cTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCa                                 >  1:99453/1‑139 (MQ=255)
                                                                                                  cTGAATGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCAc                                >  2:73525/1‑140 (MQ=255)
                                                                                                     aaTGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCAcgcg                             >  1:469780/1‑140 (MQ=255)
                                                                                                       tGTAACGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGa                           <  1:73525/140‑1 (MQ=255)
                                                                                                           aCGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGc                       >  1:469619/1‑140 (MQ=255)
                                                                                                           aCGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGc                       >  1:106917/1‑140 (MQ=255)
                                                                                                           aCGCGGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGc                       >  1:831448/1‑140 (MQ=255)
                                                                                                            cgcgGATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCt                      <  2:106917/140‑1 (MQ=255)
                                                                                                               ggATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGa                           >  1:209380/1‑132 (MQ=255)
                                                                                                               ggATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGa                           <  2:209380/132‑1 (MQ=255)
                                                                                                               ggATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGAc                   >  2:470957/1‑140 (MQ=255)
                                                                                                                gATGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGAcc                  >  2:754398/1‑140 (MQ=255)
                                                                                                                 aTGCGCTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCa                 >  2:938895/1‑140 (MQ=255)
                                                                                                                 aTGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCa                 >  2:876263/1‑140 (MQ=255)
                                                                                                                   gcgcTGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGt               <  1:302308/140‑1 (MQ=255)
                                                                                                                       tGGATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCt           >  2:936805/1‑140 (MQ=255)
                                                                                                                        ggATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCtt          <  2:469780/140‑1 (MQ=255)
                                                                                                                         gATCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTc         >  2:588165/1‑140 (MQ=255)
                                                                                                                          aTCTTCTTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATACTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCa        <  2:904698/140‑1 (MQ=255)
                                                                                                                           tcttctTCCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTTCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAg       <  2:600980/140‑1 (MQ=255)
                                                                                                                               cttcCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACAGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTAcc   >  1:508519/1‑140 (MQ=255)
                                                                                                                                ttcCTCCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCACTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCg  <  1:52543/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                       
CAATAACGTACGGGCAAATGAACTTCGTGGCGAGAAGCGCAATCGCCTCATGCTTTAGAGCCGTCCGGTACAAAGACGTAGCCCAGACCCCAGACGGTCTGAATGTAACGCGGATGCGCTGGATCTTCTTCCACCATGCGGCGCAGACGCGAAATCTGCACGTCGATGGAGCGTTCCATTGCGGAATATTCACGACCACGGGCAAGGTTCATCAGCTTATCGCGGGAGAGCGGCTCACGCGGATGGCTGACCAGTGCCTTCAGTACCG  >  NC_000913/3535818‑3536085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑